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  4. Human inter-alpha-trypsin inhibitor heavy chain H1(ITIH1) ELISA Kit

Human inter-alpha-trypsin inhibitor heavy chain H1(ITIH1) ELISA Kit

  • 中文名称：
    人间α-胰蛋白酶抑制因子重链H1(ITIH1)酶联免疫试剂盒
  • 货号：
    CSB-E13743h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ITIH1 ELISA Kit was designed for the quantitative measurement of Human ITIH1 protein in serum, plasma, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL .

  • 别名：
    H1P ELISA Kit; IATIH ELISA Kit; IGHEP1 ELISA Kit; Inter alpha (globulin) inhibitor H1 ELISA Kit; Inter alpha (globulin) inhibitor H1 polypeptide ELISA Kit; Inter alpha trypsin inhibitor heavy chain 1 ELISA Kit; Inter alpha trypsin inhibitor heavy chain H1 ELISA Kit; Inter-alpha-inhibitor heavy chain 1 ELISA Kit; Inter-alpha-trypsin inhibitor complex component III ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H1 ELISA Kit; ITI heavy chain H1 ELISA Kit; ITI-HC1 ELISA Kit; ITIH ELISA Kit; ITIH1 ELISA Kit; ITIH1_HUMAN ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
  • 缩写：
    ITIH1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, urine, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %86
    Range %80-92
    1:200Average %97
    Range %91-105
    1:400Average %99
    Range %92-110
    1:800Average %97
    Range %86-98
  • 回收率：
    The recovery of human ITIH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-98
    EDTA plasma (n=4)9790-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.400 2.418 2.409 2.328
    5002.088 1.999 2.004 1.923
    2501.562 1.555 1.559 1.478
    1251.228 1.211 1.220 1.139
    62.50.689 0.665 0.677 0.596
    31.250.452 0.436 0.444 0.363
    15.60.270 0.254 0.262 0.181
    00.084 0.078 0.081 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.; Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.
  • 基因功能参考文献：
    1. Furthermore, thrombin treatment of the inflammatory matrix leads to dissolution of HC-HA cable structures and abolishes leukocyte adhesion. These data establish a novel mechanism whereby thrombin cleavage of HC1 regulates the adhesive properties of an inflammatory HA matrix. PMID: 27679489
    2. Data suggest that Mg2+ or Mn2+ (but not Ca2+) induce a conformational change in inter-alpha-inhibitor (ITIH1 and ITIH2) and a bikunin/chondroitin sulfate-dependent increase in thermodynamic stability; bikunin binds adjacent to the two heavy chains. PMID: 26728454
    3. The results of this study revealed that the shared genetic risk factors of both schizophrenia and major depressive disorder exist in ITIH family genes in the Han Chinese population. PMID: 24389398
    4. Data indicate that TSG-6-mediated cross-linking of hyaluronan (HA) films is impaired in the presence of inter-alpha-inhibitor (IalphaI) and that this effect suppresses the TSG-6-mediated enhancement of HA binding to CD44-positive cells. PMID: 24005673
    5. Human inter-alpha-inhibitor is a substrate for factor XIIIa and tissue transglutaminase. PMID: 21939789
    6. Transfection into H460M Human large cell lung carcinoma line decreased tumor metastasis in nude mice PMID: 11807786
    7. PTX3 directly interacts with inter-alpha-trypsin inhibitor and has a role in hyaluronan organization and cumulus oophorus expansion PMID: 17675295
    8. The first systematic analysis on the differential expression of ITIH genes in human cancer, showing frequent downregulation that may be associated with initiation and/or progression of these malignancies. PMID: 18226209

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ITIH family
  • 数据库链接：

    HGNC: 6166

    OMIM: 147270

    KEGG: hsa:3697

    STRING: 9606.ENSP00000273283

    UniGene: Hs.420257