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  4. Human mast cell tryptase,MCT ELISA Kit

Human mast cell tryptase,MCT ELISA Kit

  • 中文名称：
    人肥大细胞类胰蛋白酶(MCT)酶联免疫试剂盒
  • 货号：
    CSB-E09012h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human MCT ELISA Kit was designed for the quantitative measurement of Human MCT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .

  • 别名：
    TPSAB1 ELISA Kit; TPS1 ELISA Kit; TPS2 ELISA Kit; TPSB1 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit; Tryptase-1 ELISA Kit; EC 3.4.21.59 ELISA Kit; Tryptase I ELISA Kit; Tryptase alpha-1 ELISA Kit
  • 缩写：
    MCT
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %93
    Range %85-101
    1:4Average %96
    Range %90-102
    1:8Average %99
    Range %90-109
  • 回收率：
    The recovery of human MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9485-100
    EDTA plasma (n=4)9895-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.608 2.600 2.604 2.474
    501.765 1.696 1.731 1.601
    251.153 1.144 1.149 1.019
    12.50.687 0.667 0.677 0.547
    6.250.421 0.397 0.409 0.279
    3.120.258 0.247 0.253 0.123
    1.560.187 0.183 0.185 0.055
    00.132 0.128 0.130 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates.
  • 基因功能参考文献：
    1. Basal serum tryptase in children with actual drug hypersensitivity is not a risk factor for severe systemic reactions PMID: 27288661
    2. Increased TPSAB1 copy number is associated with multisystem disorder. PMID: 27749843
    3. this study shows that active cow's milk-induced enterocolitis syndrome children have higher serum tryptase levels compared to resolved disease PMID: 27545065
    4. sBT levels may have a potential use in supporting a diagnosis of asthma in children and as a predictor of disease severity. PMID: 28049209
    5. CD3 showed a positive correlation with tryptase and microvascular density, while multiple regression analysis efficaciously predicted microvascular density depending on CD3 and tryptase as predictors, supporting a complex interplay between these cells in sustaining tumor angiogenesis in Diffuse large B cell lymphoma patients. PMID: 25957593
    6. We found the determination of serum tryptase levels over time to be a reassuring correlate of clinical improvement in pediatric mastocytosis PMID: 26044856
    7. genetic polymorphism is associated with Dengue Hemorrhagic Fever and Dengue Shock Syndrome in Vietnam and Philippines PMID: 25797204
    8. Elevated serum tryptase and ET-1 may be markers of increased coronary instability; some cytokines may be related to the extension of CAD. PMID: 26089601
    9. these data suggest that the expression of tryptase is regulated by SCF/C-KIT signaling via the ERK1/2 and p38MAPK pathways. PMID: 24806698
    10. Urine tryptase beta was increased in patients with chronic prostatitis/chronic pelvic pain syndrome compared to controls. PMID: 24726923
    11. Results demonstrated serum tryptase levels in colo-rectal cancer (CRC) patients before radical surgical resection. PMID: 24915568
    12. Circulating tryptase level is associated to obesity related parameters and has a close relation with various metabolic risk factors for subclinical atherosclerosis PMID: 24830464
    13. Alpha-tryptase gene variation is associated with levels of circulating IgE and lung function in asthma. PMID: 24372627
    14. The best criterion for discriminating IgE- and non IgE-mediated hypersensitivity reactions in anaesthesia was a tryptase value exceeding twice the basal one. PMID: 24237068
    15. With elevated tryptase level, a history of hornet sting-triggered anaphylaxis, and venom-specific IgE, a patient is at increased risk of severe life-threatening anaphylactic response to a future sting, as well as for having underlying clonal mastocytosis. PMID: 24565497
    16. Monitoring of serum baseline tryptase in indolent systemic mastocytosis patients is closely associated with poor prognosis disease features as well as with disease progression. PMID: 24155887
    17. The accumulated data in this review suggest that mast cells and their tryptases and chymases play important roles in tissue repair. PMID: 24507159
    18. The high density of tryptase(+) Mmast cells at invasive margins of tumours was associated with advanced stages of colorectal cancer and was strongly correlated with PAR-2 expression. PMID: 23991686
    19. As much as 82% of the variation in serum tryptase is due to genetics factors, and genetic effects overlap serum tryptase and body mass index. PMID: 24054365
    20. The identification of an abundant population of tryptase(+)/Nanog(+) cells in infantile haemangioma is novel. PMID: 23559352
    21. Our results indicate that sBT levels are associated with a higher risk of severe SR in children with insect venom hypersensitivity. PMID: 23330964
    22. Tryptase is a biomarker related to the severity of anaphylaxis. PMID: 23018683
    23. results suggest that tryptase can induce microglia activation and pro-inflammatory mediator release via PAR-2-MAPK-NF-kappa B signaling pathway, which will contribute to the development of microglia-mediated inflammation in brain PMID: 22613992
    24. Both IgE and chymase associate with diabetes status. PMID: 22194960
    25. As mast cells are an important source of VEGF, tryptase, and chymase, these findings suggest that mast cell activation and mast cell-derived mediators participate in the development of Dengue hemorrhagic fever. PMID: 22363824
    26. Mast cell tryptase stimulates production of decorin by human testicular peritubular cells PMID: 21791437
    27. Two different populations of mast cells were found in melanocytic skin; one expressing both chymase and tryptase and the other with tryptase only. PMID: 22102069
    28. During breast cancer progression the mast cells may contribute to stromal remodelling and differentiation of myofibroblasts, through tryptase released in stromal microenvironment. PMID: 21707711
    29. MCT gene may play a role in the pathogenesis of scar. PMID: 20540319
    30. enhanced tryptase staining in media and adventitia of human and mouse abdominal aortic aneurysm lesions PMID: 21493897
    31. these data show that patients suffering from active active chronic urticaria (CU) have significantly higher total tryptase levels than asymptomatic CU, atopics without CU and healthy subjects. PMID: 20718781
    32. Tryptase seems to play an important role in the control of human colonic mucosal permeability, and enhanced tryptase activity was responsible for the increased permeability of rectal mucosa in irritable bowel patients PMID: 20087660
    33. The levels of tryptase and chymase expression are greatly increased in human lung tissue of anaphylactic shock. PMID: 19697770
    34. SPAG11B/D is both a substrate and a potent inhibitor of TPSAB1 activity. PMID: 19535787
    35. mitogenic effects in human airway smooth muscle cells PMID: 11792624
    36. stability and catalytic properties mediated by residues at the S1 pocket PMID: 11876641
    37. structure of Bowman-Birk inhibitor derived peptides PMID: 11906611
    38. Proliferative action is mediated by PAR2, COX2, prostaglandins, and PPARgamma : possible relevance to human fibrotic disorders. PMID: 12397176
    39. Beta-tryptase activates peripheral blood mononuclear cells isolated from healthy donors as well as multiple sclerosis (MS) patients, inducing release of TNF-alpha, IL-6, and IL-1 beta, and is most probably an important mediator of inflammation in MS. PMID: 12742661
    40. Results indicate a key role for heparin in the activation of human betaI- and beta2-tryptase. PMID: 15567416
    41. Tryptase and chymase and protein levels were determined in mast cells in fibrosarcoma. PMID: 15638376
    42. Mast cell tryptase cleaves protease-activated receptor 2 (PAR2) on colonocytes to increase paracellular permeability of the intestine during stress and inflammation. PMID: 16027150
    43. crystal structures of both the single and the double mutant forms of recombinant human alphaI-tryptase in complex with the peptide inhibitor leupeptin, as well as the structure of the non-inhibited single mutant PMID: 16414069
    44. mast cell beta-tryptase selectively cleaves asthmatic airway smooth muscle (ASM)-derived eotaxin and RANTES and abrogates their chemotactic activities, thus providing an explanation for the eosinophil paucity in asthmatic ASM bundles PMID: 16517749
    45. Bikunin was found to localize on the cell membrane, while tryptase was in the secretary granules of the mast cells from psoriatic lesions. PMID: 17146627
    46. Mast cell tryptase secretion into rheumatoid arthritis synovial fluid is higher than Osteoarthritis synovial fluid and stimulates the proliferation of Synovial fibrobalst-like cells. PMID: 17205215
    47. The disease severity and plasma tryptase levels were not affected by the number of alpha or beta tryptase alleles in mastocytosis patients. PMID: 17449330
    48. These results suggest that the characteristic neovascularization observed in pterygium may be sustained, at least in part, by mast cells angiogenic mediators, in particular tryptase. PMID: 17635650
    49. siHTbeta is for the most part an inactive species and any active monomer is a consequence of heparin binding to siHTbeta under dilute conditions where unfavorable thermodynamics and/or kinetics restrict formation of active tetramer. PMID: 17655281
    50. Up-regulates interleukin-8 expression in airway smooth muscle cells through a protease-activated receptor(PAR)-2-independent proteolytic mechanism. PMID: 18079491

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Tryptase subfamily
  • 组织特异性：
    Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surro
  • 数据库链接：

    HGNC: 12019

    OMIM: 191080

    KEGG: hsa:7177

    STRING: 9606.ENSP00000343577

    UniGene: Hs.405479