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  4. Human matrix extracellular phosphoglycoprotein,MEPE ELISA Kit

Human matrix extracellular phosphoglycoprotein,MEPE ELISA Kit

  • 中文名称：
    人细胞外基质磷酸糖蛋白(MEPE)酶联免疫试剂盒
  • 货号：
    CSB-E09173h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human MEPE ELISA Kit was designed for the quantitative measurement of Human MEPE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

  • 别名：
    Matrix extracellular phosphoglycoprotein ELISA Kit; MEPE ELISA Kit; MEPE_HUMAN ELISA Kit; OF45 ELISA Kit; Osteoblast/osteocyte factor 45 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MEPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 89  
    Range % 84-93  
    1:10 Average % 98  
    Range % 95-102  
    1:20 Average % 84  
    Range % 80-88  
    1:40 Average % 90  
    Range % 85-94  
  • 回收率：
    The recovery of human MEPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 90-102  
    EDTA plasma (n=4) 105 99-110  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.603 2.769 2.686 2.516  
    500 1.967 2.075 2.021 1.851  
    250 1.290 1.194 1.242 1.072  
    125 0.631 0.679 0.655 0.485  
    62.5 0.476 0.506 0.491 0.321  
    31.2 0.341 0.357 0.349 0.179  
    15.6 0.254 0.246 0.250 0.080  
    0 0.170 0.169 0.170    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Promotes renal phosphate excretion and inhibits intestinal phosphate absorption. Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes. Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage. Promotes dental pulp stem cell proliferation and differentiation.
  • 基因功能参考文献：
    1. Results indicate an association of MEPE gene inactivation with decreased survival after DNA damage. PMID: 23570370
    2. Mice deficient in an ortholog of MEPE show increased bone mass and are resistant to aging-related bone loss. PMID: 12421822
    3. results indicated that MEPE appeared to play an important positive role in proliferation and osteogenesis differentiation of DPCs through interaction with downstream signals. PMID: 22341070
    4. MEPE or the MEPE-derived acidic serine aspartate-rich MEPE-associated motif peptide may contribute to decreased bone mineralization in Oncogenic osteomalacia patients. PMID: 21739089
    5. Among migraineurs with aura rs7698623 in MEPE (OR = 6.37; 95% CI 3.15-12.90; p = 2.7x10(-7)) and rs4975709 in IRX4 (OR = 5.06; 95% CI 2.66-9.62; p = 7.7x10(-7)) appeared to be associated with ischemic stroke. PMID: 21779381
    6. Sclerostin acts through regulation of the PHEX/MEPE axis at the preosteocyte stage and serves as a master regulator of physiologic bone mineralization. PMID: 21312267
    7. MEPE may play a regulatory role in periodontal ligament cell osteogenic differentiation. PMID: 19031789
    8. These results suggest that abnormal MEPE cleavage occurs when PHEX activity is deficient in humans. PMID: 20581062
    9. This study demonstrates the importance of posttranslational modification for the site-specific activity of MEPE and its ASARM peptide. PMID: 19998030
    10. The patterns of expression of FGF-23, MEPE, and DMP1 differ markedly in trabecular bone, suggesting that individual osteocytes may have specialized functions. PMID: 19679205
    11. MEPE/OF45, a new dentin/bone matrix protein and candidate gene for dentin diseases mapping to chromosome 4q21. Expressed during odontogenesis. PMID: 12489176
    12. There is evidence for a hormone/enzyme/extracellular matrix protein cascade involving fibroblastic growth factor 23 (FGF23), a phosphate-regulating gene with homologies to (PHEX) and (MEPE)--REVIEW PMID: 12791601
    13. MEPE is predominantly expressed by osteocytes in human bone, with significant expression by osteocytes within mineralized bone PMID: 15108058
    14. The results are consistent with MEPE being involved in phosphate and bone metabolism in a normal population. PMID: 15292364
    15. MEPE expression is higher when cells proliferate, whereas it is downregulated as cells differentiated PMID: 18547474
    16. MEPE is a candidate phosphatonin involved in phosphate homeostasis, acting in both the kidney and the gastrointestinal tract PMID: 19005008
    17. MEPE is expressed in salivary gland ducts. PMID: 15329369
    18. MEPE is expressed on the luminal surface of kidney proximal tubules. PMID: 15954904

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Expressed by osteoblasts. Expressed by stem cells in dental pulp. Expressed by mesenchymal cells in dental papilla and dental pulp. Expressed in teeth, specifically in decidious dentin. Expressed in ondotoblasts. Expressed in salivary glands. Secreted fro
  • 数据库链接：

    HGNC: 13361

    OMIM: 605912

    KEGG: hsa:56955

    STRING: 9606.ENSP00000354341

    UniGene: Hs.676357