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  4. Human melanoma-associated antigen 3 (MAGE-3) ELISA Kit

Human melanoma-associated antigen 3 (MAGE-3) ELISA Kit

  • 中文名称：
    人黑色素瘤相关抗原3(MAGE-3)酶联免疫试剂盒
  • 货号：
    CSB-E15769h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human MAGEA3 ELISA Kit was designed for the quantitative measurement of Human MAGEA3 protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    Antigen MZ2 D ELISA Kit; Antigen MZ2-D ELISA Kit; Cancer/testis antigen 1.3 ELISA Kit; Cancer/testis antigen family 1 member 3 ELISA Kit; CT1.3 ELISA Kit; HIP8 ELISA Kit; HYPD ELISA Kit; MAGA3_HUMAN ELISA Kit; MAGE 3 antigen ELISA Kit; MAGE family member A3 ELISA Kit; MAGE-3 antigen ELISA Kit; MAGE3 ELISA Kit; MAGEA3 ELISA Kit; MAGEA6 ELISA Kit; Melanoma antigen family A 3 ELISA Kit; Melanoma associated antigen 3 ELISA Kit; Melanoma-associated antigen 3 ELISA Kit; MGC1461 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MAGE-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %89-101
    1:2Average %95
    Range %87-99
    1:4Average %88
    Range %82-93
    1:8Average %97
    Range %91-101
  • 回收率：
    The recovery of human MAGE-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10492-107
    EDTA plasma (n=4)9688-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    401.742 1.777 1.760 1.642
    201.383 1.411 1.397 1.279
    100.860 0.907 0.884 0.766
    50.539 0.559 0.549 0.431
    2.50.353 0.344 0.349 0.231
    1.250.219 0.222 0.221 0.103
    0.6250.175 0.181 0.178 0.060
    00.117 0.119 0.118
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a as repressor of autophagy. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
  • 基因功能参考文献：
    1. rapid drop (>50%) in blood level within 4 weeks of melanoma treatment associated with long-term response to therapy PMID: 27561960
    2. the objective response rate was lower in this study than in other studies carried out in the same setting with the MAGE-A3 immunotherapeutic. Investigation of a gene signatures (GS) to predict clinical benefit to adjuvant MAGE-A3 immunotherapeutic treatment is ongoing in another melanoma study. PMID: 27502712
    3. Administering autologous CD4(+) T cells that are genetically engineered to express an MHC class II-restricted antitumor TCR that targets MAGE-A3 can be used safely in the treatment of metastatic neoplasms. PMID: 28809608
    4. MAGEA3 may be demethylated in MPNST and plexiform type neurofibroma in NF-1 patients PMID: 27422441
    5. MAGEA3 was overexpressed in colorectal cancer tissue compared with healthy colon. MAGEA3 expression positively correlated with colorectal cancer progression. PMID: 27635108
    6. Comparing the overall expression of CTAs, a decreased expression of all melanoma-associated antigens (MAGEs) post-treatment and a slightly increased expression of New York esophageal squamous cell carcinoma 1 (NY-ESO-1) was visible. The simultaneous cytoplasmic and nuclear expression of pan-MAGE or MAGE-A3/A4 correlated with reduced treatment-failure-free-survival (TFFS). PMID: 27466502
    7. MAGE-A3 expression is regulated epigenetically by promoter methylation, and that its expression contributes to gastric cell proliferation and drug sensitivity. PMID: 26868260
    8. Progression free survival and levels of MAGE-A3 expression in non-small cell lung carcinoma patients with the three modes of acquired resistance are negatively correlated. PMID: 26612451
    9. the crystal structures of MAGE-A3 and MAGE-A4 PMID: 26910052
    10. MAGEA3/6 positivity was associated with significantly better disease-free survival. PMID: 25564441
    11. Anticancer effects of adenovirus-mediated calreticulin and melanoma-associated antigen 3 expression on non-small cell lung cancer cells PMID: 25704851
    12. our findings indicated that MAGE-A3 is a novel cancer stem cell antigen in bladder cancer PMID: 25031712
    13. MAGE-A3 may serve as a potential prognostic marker for clear cell renal cell carcinoma PMID: 25120790
    14. Study describes a widespread mechanism to suppress AMPK through its ubiquitination and degradation by the cancer-specific MAGE-A3/6-TRIM28 ubiquitin ligase. MAGE-A3 and MAGE-A6 are highly similar proteins normally expressed only in the male germline but frequently re-activated in human cancers. PMID: 25679763
    15. MAGE proteins bind to KAP1, a gene repressor and ubiquitin E3 ligase which also binds KRAB domain containing zinc finger transcription factors (KZNFs), and MAGE expression may affect KZNF mediated gene regulation. PMID: 25107531
    16. Study demonstrated that the expression of MAGE-A3/4 antigen might be a valuable prognostic factor regarding survival in patients with NSCLC. PMID: 24675493
    17. Data indicate that MAGE3, Survivin and B-cell maturation antigen (BCMA) mRNA-pulsed dendritic cells (DCs) are capable of stimulating tumor-associated antigens (TAA)-specific T-cell responses in multiple myeloma (MM) patients. PMID: 23728352
    18. Cells bearing either intermediate proteasome present peptides MAGE-A(3114-122) and MAGE-C2(42-50) as efficiently as cells equipped with the immunoproteasome. PMID: 22925930
    19. High MAGE-3 gene expression is associated with metastases in hepatitis C virus patients complicated by hepatocellular carcinoma. PMID: 21452042
    20. the expression of MAGE, as confirmed in the RT-PCR analysis, could be used as an alternative method for the early diagnosis of salivary gland tumours. PMID: 22498264
    21. Report MAGEA1-A6 expression and MAGE A3 methylation in sputum suggests presence of lung cancer cells or precancerous cells. PMID: 22134685
    22. Cancer/testis antigens are novel targets of immunotherapy for adult T-cell leukemia/lymphoma. PMID: 22323448
    23. Data indicate the association of MAGE-A3 expression and poor prognosis in diffuse large B-cell lymphoma (DLBCL) patients. PMID: 22183072
    24. MAGE-A3 gene may have a clinical relevance and important role as a risk factor in the development of acute myeloid leukemia PMID: 21804405
    25. Expression of MAGE I proteins, MAGE-A3 or MAGE-C2, relieved repression of a reporter gene by ZNF382 and MAGE I expression relieved KAP1 mediated ID1 repression. PMID: 21876767
    26. we found that the SSX4 and MAGE-A3 genes are frequently expressed in brain tumor cell lines PMID: 21347689
    27. MAGE-A3 was detected in a significantly higher percentage of relapsed patients compared with newly diagnosed, establishing a novel correlation with progression of disease. PMID: 21565982
    28. MAGE-A3, a cancer-testis antigen, plays an important role in the survival of multiple myeloma cells. PMID: 20015885
    29. Primary tumors with and without lymph node metastases showed no significant differences in MAGE-A 3/4 (P=0.672) and NY-ESO-1 (P=0.444) expression PMID: 21556122
    30. RT-PCR assays of MAGE-A3 and MAGE-A4 in blood of breast cancer (BC) patients may have prognostic and predictive implications, and they are promising specific tumor markers of BC PMID: 21264495
    31. MAGE3 expression mediated by demethylation of MAGE3 promoter induce progression of non-small cell lung cancer. PMID: 21273595
    32. Molecular upstaging of MAGE-A3 using rt-pcr was correlated with prognosis in melanoma patients PMID: 21135695
    33. Report immunohistochemical expression of MAGE-A3 in renal oncocytoma and chromophobe renal cell carcinoma. PMID: 20591578
    34. results showed that MAGE-A3 gene expression was frequent in NHL patients and decreased after effective chemotherapy, suggesting that MAGE-A3 can be used as a tumor marker for circulating lymphoma cells in patients with NHL. PMID: 20036422
    35. Tumor-specific antigen MAGE may play a role in the occurrence and development of ovarian cancer and can be used as one of the important indicators for early diagnosis, efficacy evaluation and prognostic determination of ovarian cancer. PMID: 20423514
    36. MAGE-A3/6 and NY-ESO-1 were expressed in 50.0% (66/132) and 18.2% (24/132) of non-small-cell lung carcinomas, respectively. PMID: 19795170
    37. Identification of immunodominant regions among promiscuous HLA-DR-restricted CD4+ T-cell epitopes on the tumor antigen MAGE-3 PMID: 12393675
    38. This melanoma-associated marker was detected in melanoma cell lines. PMID: 12710945
    39. first evidence that the naturally processed MAGE-3(271-279) can be isolated and identified from the tumor tissue of hepatocellular carcinoma patient PMID: 15885805
    40. The newly identified MAGE-3(113)-peptide epitope is naturally processed and presented as the CTL epitope on MAGE-3-expressing GI cancer cells, indicating that anti-MAGE-3 immune targeting with the MAGE-3(113) peptide is a promising approach for treatment. PMID: 16446550
    41. MAGE-A3 gene mRNA is expressed at the mRNA level in a proportion of human leukemias. PMID: 16806467
    42. The promoter hypomethylation of MAGE-A1 and MAGE-A3 genes up-regulates their expression in colorectal carcinomas as well as in gastric cancers and might play a significant role in the development and progression of human colorectal carcinomas. PMID: 17007017
    43. Gene expression profiling identified the cancer/testis antigen MAGE-A3/6 as a novel target of FGFR2-IIIb signaling. PMID: 17699848
    44. Measurement of Melan-A, gp100, MAGE-3, MIA and tyrosinase represents a prognostic factor and a method for early detection of metastasis and treatment response of melanoma patients. PMID: 18181974
    45. MAGEC1/CT7, MAGEA3/6 and LAGE-1 are good candidates for immunotherapy, since together they cover 85% of our MM cases. PMID: 18237105
    46. the repertoire of MAGE-A3 epitopes recognized in vivo by CD4+ T cells are influenced by endosomal proteases PMID: 18316621
    47. MAGE-A3 as a target of FGFR2-IIIb and estrogen action and provide evidence for a common histone-modifying network in the control of the balance between opposing signals. PMID: 18381936
    48. MAGE-A3 may have a role in melanoma and could be used in a therapeutic vaccine PMID: 18483279
    49. MAGE A3 is a functional integrator of diverse signals, including FGFR2 and FN, to modulate cancer progression. PMID: 18829569
    50. Its expression is significantly associated with prognostic factors in poor outcome of the non-small cell lung cancer PMID: 18982744

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  • 组织特异性：
    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.
  • 数据库链接：

    HGNC: 6801

    OMIM: 300174

    KEGG: hsa:4102

    STRING: 9606.ENSP00000359301

    UniGene: Hs.417816