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  4. Human neuregulin-4 (NRG4) ELISA kit

Human neuregulin-4 (NRG4) ELISA kit

  • 中文名称：
    人神经调节蛋白4(NRG4)酶联免疫试剂盒
  • 货号：
    CSB-EL016080HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human NRG4 ELISA Kit was designed for the quantitative measurement of Human NRG4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

  • 别名：
    DKFZp779N0541 ELISA Kit; DKFZp779N1944 ELISA Kit; Heregulin 4 ELISA Kit; HRG4 ELISA Kit; Neuregulin 4 ELISA Kit; Neuregulin-4 ELISA Kit; NRG-4 ELISA Kit; NRG4 ELISA Kit; NRG4_HUMAN ELISA Kit; pro-neuregulin-4; membrane-bound isoform ELISA Kit; Pro-NRG4 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %86-95
    1:2Average %97
    Range %91-105
    1:4Average %95
    Range %90-101
    1:8Average %85
    Range %82-89
  • 回收率：
    The recovery of human NRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-96
    EDTA plasma (n=4)9894-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.335 2.256 2.296 2.102
    40001.648 1.768 1.708 1.514
    20001.191 1.201 1.196 1.002
    10000.812 0.823 0.818 0.624
    5000.588 0.596 0.592 0.398
    2500.475 0.484 0.480 0.286
    1250.367 0.381 0.374 0.180
    00.193 0.195 0.194 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors.
  • 基因功能参考文献：
    1. Nrg4 is highly and preferentially expressed in healthy adipocytes, while its expression was substantially reduced in obesity. Nrg4 activated endothelial angiogenic functions and angiogenesis both in vitro and in vivo. PMID: 29902456
    2. Serum NRG4 values were significantly elevated in the Gestational Diabetes Mellitus (GDM) group compared to the control group and is associated with metabolic parameters of GDM. PMID: 29282998
    3. circulating Nrg4 concentrations are inversely associated with subclinical atherosclerosis in obese adults; circulating Nrg4 might play a role in identifying patients at high risk for cardiovascular disease PMID: 27819316
    4. The circulating Nrg4 level is elevated in the prediabetic and diabetic patients compared to control and is an independent risk factor associated with diabetes. PMID: 27862843
    5. High serum Nrg4 levels are associated with type 2 diabetes mellitus. PMID: 27329015
    6. NRG4 and its receptor ErbB4 are present in human breast milk and developing human intestine, respectively. Thus, NRG4-ErbB4 signaling may be a novel pathway for therapeutic intervention or prevention in NEC. PMID: 25216938
    7. Nrg4 might have a protective role in the development of Non-alcoholic fatty liver disease (NAFLD). PMID: 26476959
    8. results establish Nrg4 as a brown fat-enriched endocrine factor with therapeutic potential for the treatment of obesity-associated disorders, including type 2 diabetes and nonalcoholic fatty liver disease (NAFLD). PMID: 25401691
    9. Pharmacological inhibition of PI3K/Akt signaling reversed the anti-apoptotic effects of NRG4, confirming the role of this cascade in NRG4-induced cell survival PMID: 23033483
    10. The recombinant neuregulin 4 induced the tyrosine phosphorylation of HER4. PMID: 21805036
    11. NRG4 overexpression is associated with advanced-stage prostate cancer PMID: 17545517
    12. describes a novel splice variant of the NRG4 gene, NRG4A2, which encodes a C-terminal region containing a predicted type I PDZ-binding peptide PMID: 17684490
    13. NRG4 was positive in almost all breast cancers studied. PMID: 17962208
    14. Neuregulin-4 expression is downregulated during urinary bladder cancer progression PMID: 15583696

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  • 亚细胞定位：
    [Pro-neuregulin-4, membrane-bound isoform]: Cell membrane; Single-pass type I membrane protein.; [Neuregulin-4]: Secreted.
  • 蛋白家族：
    Neuregulin family
  • 数据库链接：

    HGNC: 29862

    OMIM: 610894

    KEGG: hsa:145957

    STRING: 9606.ENSP00000378367

    UniGene: Hs.732438