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  4. Human omentin ELISA Kit

Human omentin ELISA Kit

  • 中文名称：
    人网膜素(omentin)酶联免疫试剂盒
  • 货号：
    CSB-E09745h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human ITLN1 ELISA Kit allows for the in vitro quantitative determination of ITLN1 concentrations in serum, plasma, cell culture supernates, or tissue homogenates. This kit exclusively recognizes human ITLN1. Omentin, also called intelectin-1 (ITLN1), is an adipokine with an anti-inflammatory function and is generated by the stromal-vascular fraction of visceral adipose tissue. ITLN1 has recently been identified as a secretory and galactofuranose-binding lectin and is primarily produced in the gastrointestinal goblet cells and omentum. Evidence has shown that ITLN1 is involved in the gut immune defense against microorganisms, insulin-stimulated glucose uptake, chronic obstructive pulmonary disease, and asthma. ITLN1 also participates in neoplastic diseases such as pleural mesothelioma and gastric and prostate cancer.

    The detection mechanism of this kit is based on the Sandwich-ELISA technique. ITLN1 in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled ITLN1 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with ITLN1 content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.

  • 别名：
    Endothelial lectin HL 1 ELISA Kit; Endothelial lectin HL-1 ELISA Kit; FLJ20022 ELISA Kit; Galactofuranose binding lectin ELISA Kit; Galactofuranose-binding lectin ELISA Kit; hIntL ELISA Kit; HL 1 ELISA Kit; HL-1 ELISA Kit; HL1 ELISA Kit; Intelectin ELISA Kit; Intelectin 1 ELISA Kit; Intelectin-1 ELISA Kit; Intestinal lactoferrin receptor ELISA Kit; INTL ELISA Kit; ITLN ELISA Kit; ITLN-1 ELISA Kit; ITLN1 ELISA Kit; ITLN1_HUMAN ELISA Kit; LFR ELISA Kit; Omentin ELISA Kit; OTTHUMP00000027882 ELISA Kit
  • 缩写：
    ITLN1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human omentin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:200Average %85
    Range %80-92
    1:400Average %96
    Range %92-100
    1:800Average %102
    Range %93-110
    1:1600Average %95
    Range %82-99
  • 回收率：
    The recovery of human omentin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9180-100
    EDTA plasma (n=4)9690-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.349 2.192 2.271 2.166
    501.869 1.806 1.838 1.733
    251.354 1.294 1.324 1.219
    12.50.923 0.914 0.919 0.814
    6.250.592 0.559 0.576 0.471
    3.120.322 0.299 0.311 0.206
    1.560.213 0.209 0.211 0.106
    00.109 0.101 0.105 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner. Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris. Does not bind human glycans. Probably plays a role in the defense system against microorganisms (Probable). May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption.
  • 基因功能参考文献：
    1. chemerin and leptin are elevated and omentin-1 and visfatin levels are decreased in Gestational diabetes mellitus women complicated by obesity. PMID: 29337272
    2. Study shows omentin Val/Val (mutant homozygote) genotype increases predisposition to acne vulgaris by probably disrupting overall protein function of omentin. PMID: 30301508
    3. The study showed for the first time serum omentin and visfatin levels to be decreased after lung transplantation in end-stage lung disease patients PMID: 29277835
    4. ITLN1, PPARg AND TNFa GENE EXPRESSION IN VISCERAL ADIPOSE TISSUE. PMID: 30188100
    5. Omentin may play a role in the pathophysiology of preterm births but it is not involved in the mechanism of preterm premature rupture of the membranes. PMID: 28440092
    6. Loss of Omentin-1 expression is Associated with Carotid Plaque Instability among Ischemic Stroke Patients. PMID: 29225325
    7. Val109Asp polymorphic marker of intelectin 1 gene is associated with abdominal obesity. PMID: 29482534
    8. Reduced serum omentin-1 is associated with inflammatory mechanism and insulin resistance in the pathogenesis of diabetic nephropathy in type 2 diabetes mellitus patients. PMID: 28864059
    9. The aims of this study were to evaluate and correlate circulating chemerin, apelin, vaspin, and omentin-1 levels in obese type 2 diabetic Egyptian patients with coronary artery stenosis (CAS), and to assess their usefulness as noninvasive diagnostic biomarkers. PMID: 28957639
    10. six-month lifestyle weight loss intervention significantly increased serum levels in obese children PMID: 28833541
    11. ITLN1 expression was significantly increased in asthmatic airways and in lesional skin of atopic dermatitis patients PMID: 28224996
    12. Plasma omentin-1 level was significantly higher in patients with good coronary collateral circulation (CCC) than those with poor CCC (566.57+/-26.90 vs. 492.38+/-19.70 ng/mL, p=0.024). Besides, omentin-1 was positively correlated with total cholesterol, high-density lipoprotein, and gensini score but inversely with hyperlipidemia and body mass index (all p values0.05). PMID: 28123148
    13. In conclusion, our study, which focused on hepatic FGF21 and omentin-1 mRNA expression, confirmed marked expression of both molecules in the liver of morbidly obese patients with NAFLD. PMID: 28820393
    14. Myocardial injury leads to a decrease in ITLN1 expression in the heart and a corresponding increase in plasma levels PMID: 28687077
    15. the effects of omentin-1 on KLF2 expression are mediated by p53. PMID: 29408455
    16. Omentin levels are increased in cirrhotic patients without portal vein thrombosis, but do not influence platelet activity. PMID: 28465646
    17. Omentin-1 levels are closely associated with the endogenous insulin reserve and may be used in follow-up of patients. PMID: 28073124
    18. Omentin expression increases in the epicardial adipose tissueof non-obese coronary artery disease patients, despite a decrease in plasma levels, suggesting that omentin may play a role in the pathogenesis of coronary artery disease PMID: 27450783
    19. Omentin-1 concentrations may be related to increased stroke risk PMID: 27298015
    20. A decreased level of circulating omentin negatively correlated with white blood cells and procalcitonin in patients with acute respiratory distress syndrome. PMID: 27607575
    21. Serum levels of omentin-1 were positively associated with increases in glycemia and incident type 2 diabetes in an older population. PMID: 28679518
    22. These findings suggested that elevated serum omentin levels were only very mildly related to the changes in cardiac volume and function in chronic heart failure patients PMID: 27270246
    23. Data suggest that circulating omentin-1 levels are lower in patients with PCOS (polycystic ovary syndrome) as compared to control subjects; circulating omentin-1 levels appear to correlate with insulin resistance but do not appear to correlate with overweight/obesity in these patients. [META-ANALYSIS] PMID: 27908216
    24. In conclusion, DAB2 and Intelectin-1 are newly identified positive markers of mesothelioma and have potential to be included in future immunohistochemical marker panels for differentiation of epithelioid mesothelioma from pulmonary adenocarcinoma PMID: 28394802
    25. This study showed that consumption of olive oil-rich diet tended to increase omentin and adiponectin in comparison with the usual diet. PMID: 27931137
    26. Omentin concentrations were inversely associated with MMP-3 levels in rheumatoid arthritis patients. This relationship was influenced by population origin, rheumatoid arthritis activity and erythrocyte sedimentation rate. PMID: 27476070
    27. High omentin expression is associated with Colorectal Cancer. PMID: 27216184
    28. Our results indicate that the Asp allele of Val109Asp (T allele of rs2274907) is more frequent among men with coronary artery disease than healthy men, so it is possibly a risk factor for coronary artery disease in men only. PMID: 28325076
    29. In type 2 diabetes mellitus, both chemerin and omentin enhance the body sensitivity to insulin, which results in increased glucose uptake and increased expression of the omentin gene was reported in nasal polyps and mesothelioma. PMID: 27516571
    30. High omentin expression is associated with osteoporosis. PMID: 27614458
    31. There was no difference in serum chemerin, vaspin and omentin-1 between polycystic ovary syndrome patients and healthy controls. PMID: 27225862
    32. Atorvastatin increased serum omentin-1 concentrations in patients with coronary artery disease in a dose-dependent manner. PMID: 27749321
    33. Omentin-1 might exert a negative effect on bone remodelling in girls with anorexia nervosa by inhibiting both bone formation and resorption. The OPG/sRANKL system plays an important role in the latter. PMID: 26662650
    34. Low serum Omentin-1 level is Associated with Dilated Cardiomyopathy. PMID: 27313334
    35. omentin, as compared to vaspin, seems to provide a better predictor of insulin resistance in obese individuals. PMID: 27449535
    36. Data suggest that nonlinear resistance training or aerobic interval training for 12 weeks produces no significant changes in serum levels of omentin-1 and interleukin-18 in obese men; however, during a 4-week detraining period, omentin-1 decreases significantly and IL-18 increases significantly. PMID: 27636349
    37. Omentin-1 expression in patients with coronary artery disease was lower in epicardial adipose tissue adjacent to coronary stenotic segments than non-stenotic segments PMID: 27352781
    38. omentin reduces the development of atherosclerosis by reducing inflammatory response of macrophages through the Akt-dependent mechanisms PMID: 26714927
    39. Omentin-1 levels were markedly reduced in coronary endothelium and epicardial fat but increased in plasma and atheromatous plaques (macrophages/SMCs) in coronary artery disease (CAD) patients compared with non-CAD patients PMID: 26790473
    40. The mean serum omentin-1 concentration in adolescent girls with anorexia nervosa was higher than that of controls and obese girls. PMID: 25804090
    41. Omentin levels were lower in both obese and non-obese PCOS patients compared to healthy controls. PMID: 26291814
    42. Data show that the circulating omentin-1 levels were dramatically decreased in renal cell carcinoma (RCC) patients. PMID: 26539805
    43. Although no significant difference was detected regarding omentin Val109Asp polymorphism, Val/Val genotype frequency was found to be more in patient group than control group. It may be speculated that Val/Val genotype increases the tendency for CAD. PMID: 24370683
    44. Plasma omentin-1 concentrations were decreased in non-obese polycystic ovary syndrome individuals PMID: 26514948
    45. Data suggest serum omentin/ITLN1 levels are down-regulated in elderly population (ages 61-82 years) with type 2 diabetes and polyneuropathy, independently of established risk factors of polyneuropathy; ITLN1 is positively correlated with adiponectin. PMID: 26094489
    46. Serum omentin was associated with cardiac autonomic neuropathy in type 2 diabetes patients. PMID: 26466574
    47. analysis of expression of maternal omentin-1 in humans and in the rat animal model PMID: 26144294
    48. Lower levels of serum omentin in patients with pseudoexfoliation syndrome compared with healthy subjects may support the theory of systemic nature of the disease. PMID: 25264991
    49. A decrease in omentin-1 levels could be an independent mortality risk factor in patient with diabetes on hemodialysis. PMID: 26293449
    50. This study clearly indicates that acute aerobic exercise elicits a pro-inflammatory response (e.g. CHI3L1) with a lower anti-inflammatory effect (e.g. ITLN-1) in obese individuals. PMID: 26316585

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor. Secreted.
  • 组织特异性：
    Highly expressed in omental adipose tissue where it is found in stromal vascular cells but not in fat cells but is barely detectable in subcutaneous adipose tissue (at protein level). Highly expressed in the small intestine. Also found in the heart, testi
  • 数据库链接：

    HGNC: 18259

    OMIM: 609873

    KEGG: hsa:55600

    STRING: 9606.ENSP00000323587

    UniGene: Hs.50813