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  4. Human osteonectin,ON ELISA Kit

Human osteonectin,ON ELISA Kit

  • 中文名称：
    人骨粘连蛋白(ON)酶联免疫试剂盒
  • 货号：
    CSB-E08760h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human osteonectin (ON) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human ON in different samples, including serum, plasma, saliva, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of ON bound in the initial step.

    ON, also called SPARC, is encoded by the gene SPOCK2. It is a non-collagenous calcium-binding protein of bone matrix expressed in mineralized tissues including endothelial cells and fibroblasts. ON is involved in the adhesion of osteoblasts and platelets to their extracellular matrix and in early stromal mineralization. ON exerts these effects by binding strongly to type I collagen and hydroxyapatite. It also inhibits cell proliferation through the arrest of cells in the G1 phase of the cell cycle. ON expression is frequently increased in tissue wound repair or fibrotic diseases.

  • 别名：
    and Kazal-like domains proteoglycan 2 ELISA Kit; CWCV ELISA Kit; FLJ97039 ELISA Kit; KIAA0275 ELISA Kit; SPARC/osteonectin ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2 ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan 2 ELISA Kit; SPOCK 2 ELISA Kit; Spock2 ELISA Kit; Testican 2 ELISA Kit; Testican-2 ELISA Kit; Testican2 ELISA Kit; TICN 2 ELISA Kit; TICN2 ELISA Kit; TICN2_HUMAN ELISA Kit
  • 缩写：
    SPOCK2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, saliva, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of Human ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:20Average %94
    Range %87-98
    1:40Average %96
    Range %90-102
    1:80Average %94
    Range %88-98
    1:160Average %97
    Range %92-103
  • 回收率：
    The recovery of Human ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9894-102
    EDTA plasma (n=4)8680-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.658 2.857 2.758 2.663
    252.307 2.208 2.258 2.163
    12.51.601 1.543 1.572 1.477
    6.250.847 0.867 0.857 0.762
    3.120.485 0.474 0.480 0.385
    1.560.307 0.319 0.313 0.218
    0.780.181 0.188 0.185 0.090
    00.094 0.095 0.095
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May participate in diverse steps of neurogenesis. Binds calcium.
  • 基因功能参考文献：
    1. candidate susceptibility gene for bronchopulmonary dysplasia; lung expression pattern indicates potential role in alveolarization PMID: 21836138
    2. Neurons are a major source of all of the testican family members in the normal brain. Abundant distribution of testican 2 may contribute to glioma invasion by inactivating other testican family members including N-Tes, which all inhibit MT-MMPs. PMID: 12810672
    3. purification and characterization of a recombinant testican-2 expressed in insect cells; the C1q subcomponent of complement component C1 was identified as a potential interacting partner of testican-2 PMID: 17950619
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Highly expressed in brain. Also found in lung and testis.
  • 数据库链接：

    HGNC: 13564

    OMIM: 607988

    KEGG: hsa:9806

    STRING: 9606.ENSP00000321108

    UniGene: Hs.523009