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  4. Human pro-opiomelanocortin,POMC ELISA Kit

Human pro-opiomelanocortin,POMC ELISA Kit

  • 中文名称：
    人阿黑皮素原(POMC)酶联免疫试剂盒
  • 货号：
    CSB-E09307h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human POMC ELISA Kit was designed for the quantitative measurement of Human POMC protein in serum, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL .

  • 别名：
    ACTH ELISA Kit; Adrenocorticotropic hormone ELISA Kit; Adrenocorticotropin ELISA Kit; alpha melanocyte stimulating hormone ELISA Kit; Alpha MSH ELISA Kit; Alpha-MSH ELISA Kit; beta endorphin ELISA Kit; Beta LPH ELISA Kit; beta melanocyte stimulating hormone ELISA Kit; Beta MSH ELISA Kit; Beta-LPH ELISA Kit; Beta-MSH ELISA Kit; CLIP ELISA Kit; COLI_HUMAN ELISA Kit; Corticotropin like intermediary peptide ELISA Kit; Corticotropin lipotropin ELISA Kit; Corticotropin-lipotropin ELISA Kit; Gamma MSH ELISA Kit; Gamma-LPH ELISA Kit; Gamma-MSH ELISA Kit; Lipotropin beta ELISA Kit; Lipotropin gamma ELISA Kit; LPH ELISA Kit; Melanotropin alpha ELISA Kit; Melanotropin ELISA Kit; Melanotropin beta ELISA Kit; Melanotropin gamma ELISA Kit; met enkephalin ELISA Kit; Met-enkephalin ELISA Kit; MSH ELISA Kit; NPP ELISA Kit; POC ELISA Kit; POMC ELISA Kit; pro ACTH endorphin ELISA Kit; Proopiomelanocortin ELISA Kit; Proopiomelanocortin preproprotein ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, cell culture supernates, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %90-102
    1:2Average %89
    Range %84-95
    1:4Average %95
    Range %90-100
    1:8Average %92
    Range %87-102
  • 回收率：
    The recovery of human POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-96
    EDTA plasma (n=4)10298-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.865 2.899 2.882 2.705
    25002.498 2.486 2.492 2.315
    12502.004 2.056 2.030 1.853
    6251.562 1.598 1.580 1.403
    3121.084 1.054 1.069 0.892
    1560.692 0.682 0.687 0.510
    780.324 0.357 0.341 0.164
    00.174 0.179 0.177 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Stimulates the adrenal glands to release cortisol.; Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.; Increases the pigmentation of skin by increasing melanin production in melanocytes.; Endogenous orexigenic opiate.; Endogenous opiate.
  • 基因功能参考文献：
    1. These findings show that POMC methylation status in the blood of children is associated with metabolic profiles. Therefore, we suggest that the DNA methylation status might serve as a potential epigenetic biomarkers of metabolic syndrome. PMID: 29598821
    2. Herein, we summarise evidence supporting the potential candidacy in this light of alpha-melanocyte stimulatory hormone, an endogenous peptide hormone and a breakdown product of the neuropeptide pro-opiomelanocortin PMID: 29412826
    3. Data suggest that the stress of intermittent hypoxia up-regulates expression of mRNA and protein for POMC and CART in neuronal cell lines; GATA2 and GATA3 appear to be involved in these stress mechanisms. (POMC = proopiomelanocortin; CART = cocaine- and amphetamine-regulated transcript protein; GATA2 = endothelial transcription factor GATA-2; GATA3 = T-cell-specific transcription factor GATA-3) PMID: 29275211
    4. Mutation in POMC gene is associated with severe obesity, hypothyroidism, adrenal insufficiency and abnormal reddish hair pigmentation. PMID: 29858905
    5. In both melanoma cell lines, alphaMSH determined the reduction of proliferation through the PI(4,5)P2/PLC pathway, employing PPARgamma as an effector element. These evidence could offer perspectives for new therapeutic approaches for melanoma. PMID: 29020973
    6. The human beta-endorphin amyloid was studied in detail, exploiting solid-state NMR spectroscopy and TEM analyses. PMID: 28075583
    7. POMC and MC1R were significantly lower in vitiligo lesional skin than in non-lesional skin as well as in controls and they were significantly higher in non-lesional skin than in the skin of the controls. PMID: 26437741
    8. POMC has played an extraordinary role in the development of molecular endo-crinology and has clearly not yet given up all its secrets. PMID: 27273100
    9. E2F1-mediated hPOMC transcription is a potential target for suppressing ACTH production in ectopic Cushing's syndrome. PMID: 27935805
    10. Elevated ACTH secretion is associated with Cushing's syndrome. PMID: 28328532
    11. a novel p53/POMC/Galphas/SASH1 autoregulatory positive feedback loop is regulated by SASH1 mutations to induce pathological hyperpigmentation phenotype. PMID: 27885802
    12. Data suggest that alpha-MSH enhances cell adhesion to fibronectin in primary mixed synoviocytes obtained from osteoarthritis patients; alpha-MSH reduces secretion of cytokines (TNF, IL-6 and IL-8) in such synoviocyte cultures. PMID: 27396757
    13. Data suggest that the first screening step diagnosis for pituitary adenoma was determined based upon immunohistochemical (IHC) scores for Pit-1, SF-1, and ACTH. PMID: 27227698
    14. The aim of this survey is to evaluate the association of genetic variants of melanocortin-4-receptor (MC4R), pro-opiomelanocortin (POMC), apolipoprotein E (APOE) and agouti-related protein (AGRP) with obesity in the North Indian population. PMID: 26226973
    15. The expression of Pomc mRNA was stimulated in the arcuate nucleus after skin exposure to UVB. PMID: 26513428
    16. Low circulating plasma alpha-MSH levels are associated with obesity. PMID: 26758700
    17. hsa-miR-34a can regulate the CRH/CRHR1/POMC axis and may influence body mass index. PMID: 26940669
    18. ACTH was readily detected at significantly higher levels in regions of ACTH-secreting adenomas and in normal anterior adenohypophysis compared with non-secreting adenoma and neurohypophysis. PMID: 26084546
    19. the inhibitory effects of alpha-MSH on inflammation are dependent on MC-1 R PMID: 25898835
    20. the low circadian alpha-MSH levels observed in AN integrate the adaptive profile of appetite regulation of this disease PMID: 25798605
    21. CSF POMC was higher in lean vs. overweight/obese subjects. PMID: 26152765
    22. There was a non-linear association between leukocyte telomere length and plasma ACTH after laboratory stressor in elderly adults. PMID: 25622010
    23. Synovial fluid alpha-MSH levels showed an independent and negative correlation with articular cartilage degeneration in patients with knee ACL deficiency. PMID: 26427140
    24. This study suggest the involvement of genetic variants of POMC in the outcome of antidepressant treatment in patients with MDD, and indicate the possibility of an interaction of this association with stressful life events. PMID: 25448875
    25. Term infants born with meconium-stained amniotic fluid (MSAF) and who experienced respiratory distress had significantly lower levels of ACTH and cortisol at birth compared with well term infants born with MSAF or clear amniotic fluid. PMID: 25205705
    26. POMC-positive expression is a novel, independent poor prognostic marker. PMID: 25377161
    27. the hypothalamic proopiomelanocortin neurons, have a key role in the control of food intake and energy metabolism. PMID: 24732669
    28. Report clinicopathological/immunohistochemical features of ACTH-secreting pancreatic neoplasms. PMID: 25353285
    29. This work shows that the AR and the POMC gene might functionally interact with each other and subsequently mediate craving in alcohol-dependent patients. PMID: 24271034
    30. Expanding upon previous observations of high POMC in ectopic ACTH syndrome, this study specifically demonstrates elevated POMC levels can identify occult ectopic tumors. PMID: 25013995
    31. 9-cis retinoic acid decreased the steady-state level of the ACTH precursor proopiomelanocortin. PMID: 24926820
    32. POMC SNPs (rs6713532, rs1047521, rs3754860) were not associated with antipsychotic-induced weight gain in schizophrenia patients. PMID: 24564533
    33. Data suggest that CLOCK proteins are involved in regulation of ACTH and cortisol secretion under non-stressful conditions to maintain homeostasis in the hypothalamic-pituitary-adrenal axis as well as in response to stressful conditions. [REVIEW] PMID: 23933687
    34. these results indicate that HDGF downregulation participates in POMC-induced suppression of metastasis and EMT in melanoma. PMID: 23468531
    35. ACTH induced cAMP production and increased AR nuclear labeling in immunocytochemical assays. PMID: 22842514
    36. Data suggest that optimistic attitude/psychological intervention helps cancer patients survive longer via increase in beta-endorphin neuronal suppression of stress hormone levels and activation of innate immune cells destroying tumor cells. [REVIEW] PMID: 23810011
    37. It can be concluded from this study that the basal plasma beta endorphin level was low in migraine patients PMID: 23314782
    38. human dermal fibroblasts express only truncated/alternatively spliced POMC mRNA variants PMID: 23218956
    39. Functional heterogeneity of hypothalamic arcuate nucleus (ARC) POMC neurons reveals distinct subpopulations of POMC cells activated by 5-HT2CRs and disinhibited by 5-HT1BRs. PMID: 23739976
    40. Finally, the results from our patient strongly suggest that the absence of red hair in non-European patients with early-onset obesity and ACTH deficiency does not exclude the occurrence of POMC-inactivating mutations. PMID: 22612534
    41. A POMC mutation, found in a patient with early-onset type 2 diabetes and central obesity, segregated in the family and was associated with overweight, type 2 diabetes, hypertension and coronary heart disease in the carriers. PMID: 22643178
    42. The expression of beta-endorphin is high in hypertrophic scars, it may play an important role in pruritus. PMID: 22792774
    43. POMC neurons regulate feeding and energy homeostasis by integrating long-term adiposity signals from the hypothalamus and short-term from the brainstem. PMID: 23426689
    44. Eexpression of alpha-MSH may markedly increase in the split-thickness grafted skin and correlate with its pigmentation after the skin graft. PMID: 15835810
    45. NCKX5, a natural regulator of human skin colour variation, regulates the expression of key pigment genes MC1R and alpha-MSH and alters cholesterol homeostasis in normal human melanocytes. PMID: 23224873
    46. Despite peripheral hyperleptinemia, positive energy balance is achieved during pregnancy by a relative decrease in central leptin concentrations and resistance to leptin's effects on target neuropeptides that regulate energy balance. PMID: 23118421
    47. Findings suggest that POMC exonic variants may influence risk for both substance dependence and elevated BMI, in a population-specific manner. PMID: 23028917
    48. POMC promoter-specific DNA methylation was not affected by diagnosis or nutritional status but significantly negatively associated with cigarette smoki PMID: 22398003
    49. This review summarizes the role of leptin and serotonin receptors in regulating the activity of POMC neurons, providing a model in which different melanocortin pathways regulate energy and glucose homeostasis. PMID: 22328135
    50. Genetic disruption of polysialic acid-mediated signaling limits behavioral and metabolic adaptation to the high-fat diet (HFD), as treated transgenic mice fail to normalize energy intake and show increased body weight gain after HFD challenge. PMID: 22933782

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  • 相关疾病：
    Obesity (OBESITY); Pro-opiomelanocortinin deficiency (POMCD)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    POMC family
  • 组织特异性：
    ACTH and MSH are produced by the pituitary gland.
  • 数据库链接：

    HGNC: 9201

    OMIM: 176830

    KEGG: hsa:5443

    STRING: 9606.ENSP00000264708

    UniGene: Hs.1897