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Human selenium binding protein 1(SELENBP1) ELISA Kit

  • 中文名称：
    人硒结合蛋白1 (SELENBP1)酶联免疫试剂盒
  • 货号：
    CSB-E13947h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human SELENBP1 ELISA Kit was designed for the quantitative measurement of Human SELENBP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

  • 别名：
    56 kDa selenium binding protein ELISA Kit; 56 kDa selenium-binding protein ELISA Kit; hSBP ELISA Kit; LPSB ELISA Kit; SBP ELISA Kit; SBP1 ELISA Kit; SBP1_HUMAN ELISA Kit; SBP56 ELISA Kit; Selenbp1 ELISA Kit; Selenbp2 ELISA Kit; Selenium Binding Protein 1 ELISA Kit; Selenium binding protein 2 ELISA Kit; Selenium binding protein1 ELISA Kit; Selenium-binding protein 1 ELISA Kit; SELNBP1 ELISA Kit; SP56 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SELENBP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-88
    1:2Average %97
    Range %91-105
    1:4Average %97
    Range %92-104
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of human SELENBP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9490-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.520 2.604 2.562 2.367
    40002.203 2.214 2.209 2.014
    20001.888 1.899 1.894 1.699
    10001.445 1.496 1.471 1.276
    5001.005 1.067 1.036 0.841
    2500.673 0.681 0.677 0.482
    1250.378 0.384 0.381 0.186
    00.194 0.196 0.195 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria. Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport.
  • 基因功能参考文献：
    1. High anti-SBP1 is associated with infertile women with premature ovarian failure. PMID: 27965399
    2. Downregulation of selenium-binding protein 1 is associated with lung squamous cell carcinoma. PMID: 26956891
    3. cysteine 57 is a critical determinant of SBP1 function and may play a significant role in mitochondrial function. PMID: 26593911
    4. Data indicate that loss of SBP1 may play an independent contributing role in prostate cancer progression and its levels might be useful in distinguishing indolent from aggressive disease. PMID: 25993660
    5. elevated transcript expression is widespread throughout the prefrontal cortex in schizophrenia PMID: 26241353
    6. These results have revealed a novel mechanism that SELENBP1-mediated cancer inhibition is through altering lipid/glucose metabolic signaling pathways. PMID: 25974208
    7. Decreased selenium-binding protein 1 mRNA expression is associated with renal cell carcinoma. PMID: 25227434
    8. hSP56 plays an important role in regulating HIF-1alpha, which may be one of mechanisms of hSP56 expression in suppressing the malignant characteristics of prostate cancer cells. PMID: 24874852
    9. the downregulated SELENBP1 expression was reactivated by inducing differentiation. Therefore, SELENBP1 is a potential pharmacological target for individualized CRC treatment. PMID: 24737289
    10. Decreased SELENBP1 expression is an early event in lung squamous cell cancer tumorigenesis. PMID: 23977169
    11. The expression level of SELENBP1 could be an important marker for predicting survival and effectiveness of selenium supplementation in breast cancer. PMID: 23704933
    12. These results suggest that SBP1 plays a significant role in gastric cancer cell proliferation and modulates its response to anticancer drugs PMID: 23483240
    13. SP1 is present in reduced levels in several cancer types as compared with normal tissues, and lower levels are associated with poor clinical prognosis. PMID: 20530237
    14. Glutathione peroxidase (GPx)-1 enzyme activity is inversely correlated with selenium binding protein (SBP)-1 levels in prostate tissue, based on the Gleason score. PMID: 22072582
    15. The suppression of Selenium-binding protein 1 may be a late event in gastric carcinogenesis. PMID: 20480265
    16. The 3-year survival rate of gastric cancer patients with high expression of selenium-binding protein 1 was significantly higher than that of patients with low expression PMID: 21497372
    17. down-regulation of SBP1 may play a key role in the tumorigenic process of human gastric carcinoma PMID: 20354826
    18. Decreased expression of selenium-binding protein 1 in uterine leiomyoma may indicate a role of the protein in tumorigenesis. PMID: 21143902
    19. A reduced SELENBP1 expression by immunohistochemistry in liver tissues of patients with hepatocarcinoma (HCC), is reported. PMID: 21338716
    20. evidence for recurrence of decreased copy-number of the SELENBP1 locus in three unrelated schizophrenia patients' cohorts but not in controls, raising the possibility of functional involvement of these mutations in the etiology of the disease PMID: 20615253
    21. SELENBP1 expression may be an important predictor of response to chemoprevention or chemosensitization with certain forms of selenium in esophageal tissues. PMID: 20332323
    22. SBP1 has tumor suppressor functions that are inhibited in colorectal cancer through epigenetic silencing PMID: 19924303
    23. loss of selenium-binding protein 1 associated with increasing epithelial proliferation indicates selenium-binding protein 1 is involved in tumorigenesis of ovarian and micropapillary serous borderline tumor and low-grade serous carcinoma. PMID: 19896693
    24. SBP may be involved in the initial sequential events in rapid cell outgrowth, such as determining direction of cell outgrowth and recruitment of actin monomer. PMID: 15108003
    25. changes of SELENBP1 expression in malignant ovarian cancer are an indicator of aberration of selenium/androgen pathways and may reveal prognostic information of ovarian cancer PMID: 16380993
    26. Suppression of SELENBP1 is a frequent and late event in colorectal carcinogenesis, and may contribute to the rapid progression of colorectal carcinoma. PMID: 16645984
    27. Elevated SELENBP1 is a possibly consistent feature in the schizophrenic brain and this could underlie some commonalities of psychosis across the boundaries of diagnoses. PMID: 18163446
    28. Associated with differentiation of the normal colonic epithelia and may be a positive prognostic factor for survival in stage III colorectal carcinoma PMID: 18435490
    29. VDU1 was identified as a protein partner of hSP56. PMID: 19118533
    30. The genetic variation in SELENBP1 may influence risk for the disorder, while this significance did not remain when other inheritance models were considered. PMID: 19596560

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Selenium-binding protein family
  • 组织特异性：
    Widely expressed. Highly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated i
  • 数据库链接：

    HGNC: 10719

    OMIM: 604188

    KEGG: hsa:8991

    STRING: 9606.ENSP00000357861

    UniGene: Hs.632460