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Human syndecan 4 (SDC4) ELISA kit

  • 中文名称:
    人多配体聚糖4(SDC4)酶联免疫试剂盒
  • 货号:
    CSB-EL020891HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Human SDC4 ELISA Kit was designed for the quantitative measurement of Human SDC4 protein in serum, plasma, cell lysates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 缩写:
    SDC4
  • Uniprot No.:
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, cell lysates, tissue homogenates
  • 检测范围:
    0.625 ng/mL-40 ng/mL
  • 灵敏度:
    0.156 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human SDC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %85-95
    1:2Average %92
    Range %90-96
    1:4Average %93
    Range %85-100
    1:8Average %94
    Range %86-98
  • 回收率:
    The recovery of human SDC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-99
    EDTA plasma (n=4)9488-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.859 2.783 2.821 2.647
    202.037 2.005 2.021 1.847
    101.245 1.304 1.275 1.101
    50.765 0.782 0.774 0.600
    2.50.501 0.511 0.506 0.332
    1.250.349 0.353 0.351 0.177
    0.6250.261 0.257 0.259 0.085
    00.175 0.172 0.174
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

平均分:
5.0分 - 1 个评价

样品类型:血浆(抗凝剂) EDTA

样品信息:人

稀释比:没有稀释

产品评价: ELISA结果正常组和患病组有明显差异

By 刘老师

靶点详情

  • 功能:
    Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
  • 亚细胞定位:
    [Isoform 1]: Membrane; Single-pass type I membrane protein. Secreted.; [Isoform 2]: Secreted.
  • 蛋白家族:
    Syndecan proteoglycan family
  • 组织特异性:
    Expressed in epithelial and fibroblastic cells.
  • 数据库链接:

    HGNC: 10661

    OMIM: 600017

    KEGG: hsa:6385

    STRING: 9606.ENSP00000361818

    UniGene: Hs.632267