Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human thrombomodulin,TM ELISA Kit

Human thrombomodulin,TM ELISA Kit

  • 中文名称：
    人血栓调节蛋白(TM)酶联免疫试剂盒
  • 货号：
    CSB-E07937h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        THBD (Thrombomodulin)是一种由血管内皮细胞表达的跨膜糖蛋白，主要参与血液凝固和纤溶过程中的调节。THBD能够与凝血酶形成复合物，增强凝血酶对蛋白C的活化，从而抑制血栓形成。此外，THBD还参与炎症反应、血管生成、肿瘤发生等生物学过程。
        j9九游会登录入口首页生物所提供的Human thrombomodulin,TM ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的THBD，其灵敏度为0.078 ng/mL，检测范围为0.312 ng/mL-20 ng/mL。
     
  • 别名：
    THBD; THRM; Thrombomodulin; TM; Fetomodulin; CD antigen CD141
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Please I need a quotation for this devices with this specifications:product name:human soluble thrombomodulin ELISA kit,solid phases sandwiches ELISA,sample type:serum,volume required:10ul of serum,sensitivity:<10pg/ml,specificity:natural human soluble thrombomodulin,pack size:96 wells/kit polystyrene microplate (12 strips of 8 well) coated with a monoclonal antibody specific for natural human soluble thrombomodulin,assay range:62.5-4.000 pg/ml

A:
Thanks for your enquiry. I will send you the kit manual and the contact information of distributor in your area by email.
If any questions,pls let me know.
Q:

If the human Thrombomodulin ELISA kit (https://www.cusabio.com/ELISA-Kit/Human-thrombomodulin-TM-ELISA-Kit-108098.html) detects soluble Thrombomodulin fragments, or just the integral plasma membrane Thrombomodulin, or both?

A:
Thanks for your inquiry.
CSB-E07937h is designed according to this information: https: / / www.uniprot.org / uniprot / P07204.
It detects the soluble thrombomodulin.
And dear, even if the integral plasma membrane is under the case of physical or mechanical forces, it will be converted as soluble form.
So this kit actually can detect both of the forms.

靶点详情

  • 最新研究进展：
    THBD，也称为血小板凝集素受体，是一种调节血栓形成和溶解的蛋白质。最新研究表明，THBD在心脏疾病、肾脏疾病和糖尿病等疾病中发挥着重要的作用。例如，研究表明，THBD在心肌梗死和心力衰竭的预后中起着重要作用。另一方面，研究还发现，通过调节THBD的表达和功能可以治疗肾脏疾病和糖尿病等疾病。
  • 功能：
    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • 基因功能参考文献：
    1. Thrombomodulin gene C1418T polymorphism is associated with Kawasaki disease. PMID: 30008974
    2. SETD1A contributes to retinoic acid -induced thrombomodulin expression in vascular endothelial cells by modulating the activity and expression of KLF4. PMID: 29940355
    3. we for the first time demonstrated that TM binds to GPR15 via its EGF-like domain and exerts angiogenesis and cytoprotective function in vascular ECs. PMID: 28386128
    4. Bioinformatics analysis and screening of controls strongly suggested that the THBD-p.Trp153Gly mutation might be related to RPL aetiology. PMID: 29195508
    5. Taken together, our findings suggest that TM-PKCdelta interaction may contribute to cardiovascular disorders by affecting monocye differentiation, which may develop future therapeutic applications. PMID: 27910925
    6. A heterozygous variant displaying autosomal dominant inheritance (c.1611 C>A) was found in the gene THBD which encodes the glycoprotein thrombomodulin. This sequence change results in a stop codon (p.Cys537Stop) and truncation of the protein PMID: 28267383
    7. Lys 42, Lys 43, Lys 44 and Arg 12 are critical for the interaction of TAFI with the thrombin-thrombomodulin complex, which modulates its antifibrinolytic potential. PMID: 28640323
    8. Ligation of anti-HLA class I and II antibodies produces different effects on the endothelial expression of TBM and on serum levels of TBM in transplant recipients. PMID: 28239987
    9. Fibrinogen gamma acts as thrombomodulin II. (Review) PMID: 27784620
    10. Thrombomodulin (TM) promotes angiogenesis by enhancing cell adhesion, migration, and FAK activation through interaction with fibronectin. PMID: 27602495
    11. The elevation of serum thrombomodulin (sTM)level suggests that endothelial damage occurs in Abdominal Aortic Aneurysm pathogenesis. PMID: 28473982
    12. This population-based cohort study within the ARIC study did not replicate the Hernandez et al. finding that carrying the minor allele of 3 THBD SNPs doubles the risk of venous thromboembolism in African Americans. In fact, the HRs of VTE among carriers of the minor allele were <1. HRs were similar for white subjects. A strand-flip did not explain the discrepancies. PMID: 28619983
    13. these results suggest a novel function for thrombomodulin as an adhesion molecule in monocytes, where it enhances cell adhesion by binding Ley, leading to beta2 integrin activation via p38 MAPK PMID: 27808085
    14. TM, especially TME45, maintains vascular integrity, at least in part, via Src signaling. PMID: 27643869
    15. The present study found that the fifth epidermal growth factor-like domain of thrombomodulin (TME5) possesses the cytoprotective function in association with an increase in levels of anti-apoptotic myeloid cell leukemia-1 protein in an activated protein C-independent PMID: 27427915
    16. Case Report: CD141+ myeloid dentritic cell differentiation of a juvenile myelomonocytic leukemia. PMID: 28414089
    17. the effect of thromobomodulin c.1418C > T polymorphism on the pathogenesis of venous thrombosis PMID: 28710034
    18. The finding of a previously unrecognized fibrinolytic phenotype indicates that bleeding in Thrombomodulin-associated coagulopathy has a complex pathogenesis and highlights the pivotal role of TM as a regulator of hemostasis. PMID: 27436851
    19. TM mediates cell proliferation and migration via the Epithelial-To-Mesenchymal Transition (EMT) biomarkercyclooxygenase (COX)-2 PMID: 27512995
    20. the whole THBD gene sequenced in patients with recurrent venous thromboembolism (VTE); found 8 polymorphisms in the THBD gene in Swedish population; none of these polymorphisms was significantly associated with the risk of VTE recurrence; results indicate that THBD polymorphisms may not be a risk factor for VTE recurrence PMID: 28049360
    21. CORM-2 protects human umbilical vein endothelial cells from lipopolysaccharide-induced injury, by way of suppressing NF-kappaB activity, which downregulates TM and EPCR mRNAs. It also decreases MMP-2 expression and prevents the shedding of TM and EPCR from the surface of endothelial cells, so as to preserve their protective effect. PMID: 28538400
    22. The results demonstrate that the LFA-1 and Mac-1 integrins on leukocytes bind to thrombomodulin (TM), thereby establishing the molecular and structural basis underlying LFA-1 and Mac-1 integrin interaction with TM on endothelial cells. PMID: 27055590
    23. Human thrombomodulin transgenic aortic endothelial cells are less sensitive to activation by either HMGB1 or hTNFalpha, an effect that appears to be dependent on the lectin-like domain of TBM. PMID: 27077599
    24. In placenta of patients with preeclampsia, we detected abnormal expression of F3 and THBD with increased protein and mRNA levels. The role of these molecules in the pathogenesis of this disease and in alterations of hemostatic and histopathological aspects of placentas need further studying. PMID: 27002259
    25. recombinant TM (Solulin) can protect the intestine from toxicity in a clinically relevant rat model PMID: 27459702
    26. TM up-regulated E-cadherin but down-regulated N-cadherin expression, resulting in reversal of epithelial-mesenchymal transition (EMT) in the lung cancer cells. PMID: 27223053
    27. High serum thrombomodulin expression is associated with non-alcoholic fatty liver disease. PMID: 26959535
    28. results do not suggest a predictive role for THBD c.1418C>T polymorphism in VTE recurrence PMID: 26743062
    29. R12 is a critical residue for the activation of TAFI by thrombin-thrombomodulin PMID: 26816270
    30. Study detected a statistically significant positive correlation between expanded disability status scale scores and thrombomodulin levels (p<0.01) and a 10% positive correlation between expanded disability status scale scores and APC levels in multiple sclerosis patients PMID: 27456888
    31. Case Report: thrombotic microangiopathy with mutations in complement factor I and thrombomodulin. PMID: 26613809
    32. increased plasma TM levels and serum hs-CRP levels in cerebral infarction (CI) patients were associated with the development of CI in Asians. PMID: 26133301
    33. evidence of association between the -33G/A polymorphism in the TM gene and the risk of myocardial infarction in Asians; the Ala455Val variant was not associated with atherosclerotic risk [meta-analysis] PMID: 26888356
    34. Decreased thrombomodulin expression in preeclampsia may play a role in placental dysfunction in preeclampsia and is possibly caused by an angiogenic imbalance. Hypertension and obesity are associated with thrombomodulin downregulation. PMID: 26891741
    35. The presence of THBD proximal promoter polymorphisms do not explain variations in levels of serum and cell-expressed THBD in premature acute coronary syndrome patients in Bahrain. PMID: 26226255
    36. the functional relevance of the rs3176123 variation and indicate that higher thrombomodulin expression by individuals with the 2729C allele likely accounts for their decreased risk for acute GVHD development and subsequent mortality. PMID: 26246110
    37. The lack of any association between the sTM levels and genetic variants in ARDS suggests that the increased levels of sTM may reflect severity of endothelial damage rather than genetic heterogeneity. PMID: 25643902
    38. Identified Nur77/Nor1 as novel regulators of thrombomodulin expression and function in vascular endothelial cells. PMID: 26634653
    39. The results of this study supported the association of the epistatic interactions of ALOX5AP, THBD, and KNG1 and present novel evidence for the main effect of KNG1 gene on IS susceptibility. PMID: 26159646
    40. The EGF5, 6 domains of thrombomodulin appear to be the major domains for down-regulating the complement system rather than the lectin-like domain during xenogenic stimuli. PMID: 26179123
    41. A minimal TM fragment consisting of the fourth, fifth, and most of the sixth EGF-like domain (TM456m) that has been prepared has much improved solubility, thrombin binding capacity, and anticoagulant activity versus those of previous TM456 constructs. PMID: 26468766
    42. Data indicate that blood dendritic cell antigen 3 BDCA3(+) and C-type lectin domain family 9, member A CLEC9A(+) dendritic cells (DC) are of major importance in the induction of anti-viral and anti-tumor immunity. PMID: 24910448
    43. Recombinant thrombomodulin does not impair neutrophil functions. PMID: 25214376
    44. levels of protein C and soluble thrombomodulin in critically ill patients with acute kidney injury PMID: 25790110
    45. Membrane-bound TM in macrophages plays an essential role in the development of abdominal aortic aneurysms by enhancing proinflammatory mediator elaboration, macrophage recruitment, and oxidative stress. PMID: 26338301
    46. Cyclic strain strongly downregulated TM expression in a p38- and receptor tyrosine kinase-dependent manner in aortic endothelial cells. PMID: 25238231
    47. kinetics of the interaction between serine/threonine-rich domain of thrombomodulin (rTMD23) and FGFR1 were analysed in umbilical vein endothelial cells PMID: 25388665
    48. Thrombomodulin is differentially regulated within cultured brain microvascular endothelial cells by cytokines and shear stress. PMID: 25250518
    49. Review/Meta-analysis: TM -33G/A and Ala455Val polymorphisms were risk factors for coronary artery disease. PMID: 25144670
    50. function and regulation of BDCA3 expression and IFN-lambda production by dendritic cells PMID: 25616220

    显示更多

    收起更多

  • 相关疾病：
    Thrombophilia due to thrombomodulin defect (THPH12); Hemolytic uremic syndrome atypical 6 (AHUS6)
  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Endothelial cells are unique in synthesizing thrombomodulin.
  • 数据库链接：

    HGNC: 11784

    OMIM: 188040

    KEGG: hsa:7056

    STRING: 9606.ENSP00000366307

    UniGene: Hs.2030