Human type Ⅱ collagen helical peptide,HELIX-Ⅱ ELISA Kit
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中文名称:人Ⅱ型胶原螺旋肽(HELIX-Ⅱ)酶联免疫试剂盒
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货号:CSB-E09708h
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:
This Human HELIXⅡ ELISA Kit was designed for the quantitative measurement of Human HELIXⅡ protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.312 ng/mL .
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别名:type 2 collagen helical peptide,HELIX-2,_x000D_ type II collagen helical peptide,HELIX-II
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缩写:HELIXⅡ
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, urine
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检测范围:1.25 ng/mL-80 ng/mL
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灵敏度:0.312 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Others
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human HELIX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-99 1:2 Average % 89 Range % 83-94 1:4 Average % 99 Range % 94-103 1:8 Average % 104 Range % 100-112 -
回收率:
The recovery of human HELIX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 84 80-88 EDTA plasma (n=4) 91 86-97 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 80 2.178 2.114 2.146 2.043 40 1.674 1.581 1.628 1.525 20 1.116 1.061 1.089 0.986 10 0.626 0.603 0.615 0.512 5 0.423 0.407 0.415 0.312 2.5 0.252 0.239 0.246 0.143 1.25 0.193 0.184 0.189 0.086 0 0.104 0.101 0.103 -
数据处理:
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货期:3-5 working days