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Human type Ⅱ collagen helical peptide,HELIX-Ⅱ ELISA Kit

  • 中文名称:
    人Ⅱ型胶原螺旋肽(HELIX-Ⅱ)酶联免疫试剂盒
  • 货号:
    CSB-E09708h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Human HELIXⅡ ELISA Kit was designed for the quantitative measurement of Human HELIXⅡ protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.312 ng/mL .

  • 别名:
    type 2 collagen helical peptide,HELIX-2,_x000D_ type II collagen helical peptide,HELIX-II
  • 缩写:
    HELIXⅡ
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, urine
  • 检测范围:
    1.25 ng/mL-80 ng/mL
  • 灵敏度:
    0.312 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human HELIX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-99  
    1:2 Average % 89  
    Range % 83-94  
    1:4 Average % 99  
    Range % 94-103  
    1:8 Average % 104  
    Range % 100-112  
  • 回收率:
    The recovery of human HELIX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 84 80-88  
    EDTA plasma (n=4) 91 86-97  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    80 2.178 2.114 2.146 2.043  
    40 1.674 1.581 1.628 1.525  
    20 1.116 1.061 1.089 0.986  
    10 0.626 0.603 0.615 0.512  
    5 0.423 0.407 0.415 0.312  
    2.5 0.252 0.239 0.246 0.143  
    1.25 0.193 0.184 0.189 0.086  
    0 0.104 0.101 0.103    
  • 数据处理:
  • 货期:
    3-5 working days

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