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  4. Human very low density lipoprotein(VLDL)ELISA Kit

Human very low density lipoprotein(VLDL)ELISA Kit

  • 中文名称：
    人极低密度脂蛋白(VLDL)酶联免疫试剂盒
  • 货号：
    CSB-E17087h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human VLDL ELISA Kit is used to quantitatively measure human VLDL concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the competitive ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops negatively to the amount of VLDL in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

    VLDL is made by the liver and released into the bloodstream. It is a lipoprotein rich in triacylglycerols (TAGs). VLDL is commonly regarded as bad cholesterol. The body needs some VLDL to run properly. VLDL carries different types of fats, primarily circulatory triglycerides, to cells and tissues. However, excess VLDL can increase the risk of developing health conditions, including heart failure. Overproduction or delayed clearance of VLDL is considered as causing hypertriglyceridemia. VLDL is involved in cardiac remodeling but also causes ventricular function impairment. VLDL engagement with its receptor VLDLR can induce the development of atherosclerosis.

  • 别名：
    N/A
  • 缩写：
    VLDL
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 μg/mL-50 μg/mL
  • 灵敏度：
    0.195 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %97
    Range %91-103
    1:400Average %95
    Range %91-99
    1:800Average %94
    Range %88-98
    1:1600Average %105
    Range %101-109
  • 回收率：
    The recovery of human VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-95
    EDTA plasma (n=4)10398-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    500.086 0.088 0.087
    250.140 0.146 0.143
    12.50.229 0.234 0.232
    6.250.338 0.348 0.343
    3.120.576 0.554 0.565
    1.560.991 0.927 0.959
    0.781.392 1.354 1.373
    02.568 2.684 2.626
  • 数据处理：
  • 货期：
    3-5 working days

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