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  4. Human α2-Antiplasmin,α2-AP ELISA Kit

Human α2-Antiplasmin,α2-AP ELISA Kit

  • 中文名称：
    人α2抗纤溶酶(α2-AP)酶联免疫试剂盒
  • 货号：
    CSB-E09452h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human SERPINF2 ELISA Kit was designed for the quantitative measurement of Human SERPINF2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

  • 别名：
    A2AP ELISA Kit; A2AP_HUMAN ELISA Kit; AAP ELISA Kit; Alpha 2 antiplasmin ELISA Kit; Alpha 2 antiplasmin pigment epithelium derived factor ELISA Kit; Alpha 2 AP ELISA Kit; ALPHA 2 PI ELISA Kit; Alpha 2 plasmin inhibitor ELISA Kit; Alpha 2 plasmin inhibitor deficiency ELISA Kit; Alpha-2-antiplasmin ELISA Kit; Alpha-2-AP ELISA Kit; Alpha-2-PI ELISA Kit; Alpha-2-plasmin inhibitor ELISA Kit; Antiplasmin deficiency ELISA Kit; API ELISA Kit; Plasmin inhibitor deficiency ELISA Kit; PLI ELISA Kit; Serine (or cysteine) peptidase inhibitor; clade F; member 2 ELISA Kit; Serine (Or cysteine) peptidase inhibitor; clade F; member 2; isoform CRA_c ELISA Kit; Serine (or cysteine) proteinase inhibitor; clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 ELISA Kit; Serine (Or cysteine) proteinase inhibitor; clade F; member 2 ELISA Kit; Serine or cysteine peptidase inhibitor clade F member 2 ELISA Kit; Serpin F2 ELISA Kit; Serpin peptidase inhibitor clade F ELISA Kit; Serpin peptidase inhibitor; clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 ELISA Kit; SERPINF2 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human α2-AP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %84-93
    1:2Average %85
    Range %81-91
    1:4Average %92
    Range %88-100
    1:8Average %95
    Range %91-99
  • 回收率：
    The recovery of human α2-AP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8983-94
    EDTA plasma (n=4)9590-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.272 2.346 2.309 2.214
    5001.476 1.491 1.484 1.389
    2500.863 0.892 0.878 0.783
    1250.448 0.479 0.464 0.369
    62.50.268 0.251 0.260 0.165
    31.20.187 0.178 0.183 0.088
    15.60.169 0.159 0.164 0.069
    00.097 0.092 0.095 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
  • 基因功能参考文献：
    1. alpha2AP has a profibrotic effect probably not by the action as a plasmin inhibitor, and the blocking of alpha2AP exerts an antifibrotic effect in humans and mice with systemic sclerosis PMID: 26743600
    2. This review presents recent findings regarding the main aspects of the natural heterogeneity of A2AP with particular focus on the functional and possible clinical implications. [review] PMID: 26626994
    3. Possession of the alpha2AP 407Lys allele was negatively associated with AAA, and thus changes in alpha2AP may affect aneurysm growth and development. PMID: 25065555
    4. Changes in plasma A2AP are associated with cardiovascular disease event presentation. PMID: 26088309
    5. A significant correlation was found between soluble fibroblast activation protein levels and alpha2-antiplasmin cleavage in coronary thrombosis. PMID: 25464232
    6. increased N-terminal cleavage of alpha2AP may have a role in liver cirrhosis PMID: 24034420
    7. FXIII-A has a functional role through exposure on the activated platelet membrane where it exerts antifibrinolytic function by cross-linking alpha2AP to fibrin PMID: 25331118
    8. Data suggest serum A2AP (SERPINF2) level can serve as biomarker for diabetic retinopathy; levels of A2AP (but not fibrinogen, plasminogen, or plasminogen activator inhibitor 1) are up-regulated in hyperglycemic type 1 diabetes with retinopathy. PMID: 24818165
    9. protective mediator during gram-negative (pneumo)sepsis, limiting bacterial growth, inflammation, tissue injury, and coagulation PMID: 23992406
    10. Study revealed that plasmin was present in tumor tissue, and that it was responsible for processing progalanin to galanin(1-20) in the extracellular environment. PMID: 21707521
    11. Data suggest that decreased amounts of alpha2-plasmin inhibitor in plasma vs serum ex vivo may reflect reduced factor XIII (FXIII) in vivo; thus, plasma vs serum alpha2-plasmin inhibitor may be useful diagnostic marker for severe FXIII deficiency. PMID: 22205503
    12. fibrinolysis is enhanced by inhibiting enzymatic cleavage of precursor alpha2-antiplasmin PMID: 21251197
    13. Truncation of monocyte chemoattractant protein 1 by plasmin promotes blood-brain barrier disruption. PMID: 21486949
    14. Levels of free full-length alpha2AP were decreased in myocardial infarction (MI); the percentage of C-terminally cleaved alpha2AP was unaltered, and Arg407Lys did not influence alpha2AP levels or MI risk. PMID: 21505192
    15. the antifibrinolytic function of FXIII is independent of fibrin-fibrin cross-linking and is expressed exclusively through alphaAP. PMID: 21471521
    16. ADAMTS13 inactivation by plasmin as a potential cause of thrombotic thrombocytopenic purpura PMID: 20553378
    17. deficiency in patients with Philadelphia chromosome-positive haematologic cancer receiving imatinib mesylate PMID: 19492163
    18. OPN is highly susceptible to cleavage near its integrin-binding motifs, and the protein is a novel substrate for plasmin and cathepsin D PMID: 20071328
    19. FXIIIa substrate, A2AP, sequences suggests that the residue located two positions beyond the reactive glutamine is not conserved. this position makes important contributions to effective FXIIIa-A2AP interactions PMID: 19691486
    20. Multiple Lys residues within alpha 2-antiplasmin contribute, perhaps in a zipper-like fashion, to its binding to the in-tandem, multikringle array that configures the plasmin heavy chain. PMID: 12549929
    21. Alpha2-antiplasmin has an important role in acute pulmonary embolism PMID: 12911586
    22. the Arg6Trp polymorphism may play a significant role in governing the long-term deposition/removal of intravascular fibrin PMID: 17317851
    23. Fibrinolysis is amplified by converting alpha-antiplasmin from a plasmin inhibitor to a substrate PMID: 17883703
    24. Hydroxyethyl starch (HES) dilution enhances fibrinolysis by diminishing alpha2-antiplasmin-plasmin interactions. PMID: 17890952
    25. Sequencing analysis revealed the presence of two alpha2-PI gene variations, both in the second half of exon 10: a frameshift mutation and a G to A transition at nucleotide 11337 in codon 407. PMID: 17961166
    26. Thrombin activatable fibrinolysis inhibitor and alpha(2)-antiplasmin are not markers for recanalization in patients with ischemic stroke treated with recombinant tissue-type plasminogen activator. PMID: 18048863
    27. Plasmin in nephrotic urine activates the epithelial sodium channel PMID: 19073825

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  • 相关疾病：
    Alpha-2-plasmin inhibitor deficiency (APLID)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    HGNC: 9075

    OMIM: 262850

    KEGG: hsa:5345

    STRING: 9606.ENSP00000321853

    UniGene: Hs.159509