Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human β-defensins 2 ELISA Kit

Human β-defensins 2 ELISA Kit

  • 中文名称：
    人β-防御素2酶联免疫试剂盒
  • 货号：
    CSB-E13201h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human DEFB4 ELISA Kit was designed for the quantitative measurement of Human DEFB4 protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    BD 2 ELISA Kit; BD-2 ELISA Kit; BD2 ELISA Kit; beta 2 ELISA Kit; beta 2 Defensin ELISA Kit; Beta defensin 2 ELISA Kit; Beta defensin 4B ELISA Kit; Beta-defensin 2 ELISA Kit; Beta-defensin 4A ELISA Kit; DEF B2 ELISA Kit; DEF B4 ELISA Kit; DEFB 102 ELISA Kit; DEFB 2 ELISA Kit; DEFB 4 ELISA Kit; DEFB102 ELISA Kit; DEFB2 ELISA Kit; DEFB2; formerly ELISA Kit; DEFB4 ELISA Kit; DEFB4; formerly ELISA Kit; DEFB4B ELISA Kit; DEFB4P ELISA Kit; Defensin ELISA Kit; Defensin beta 2 ELISA Kit; Defensin; beta 4 ELISA Kit; Defensin; beta 4; pseudogene ELISA Kit; Defensin; beta 4A ELISA Kit; Defensin; beta 4B ELISA Kit; Defensin; beta; 2; formerly ELISA Kit; Defensin; beta; 4; formerly ELISA Kit; DFB4A_HUMAN ELISA Kit; HBD 2 ELISA Kit; hBD-2 ELISA Kit; HBD2 ELISA Kit; mBD-2 ELISA Kit; SAP 1 ELISA Kit; SAP1 ELISA Kit; Skin antimicrobial peptide 1 ELISA Kit; Skin-antimicrobial peptide 1 ELISA Kit
  • 缩写：
    DEFB4
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, urine, cell culture supernates, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Microbiology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %81-92
    1:2Average %94
    Range %90-100
    1:4Average %98
    Range %91-110
    1:8Average %91
    Range %85-98
  • 回收率：
    The recovery of human β-defensins 2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.821 2.752 2.787 2.689
    20002.182 2.076 2.129 2.031
    10001.438 1.364 1.401 1.303
    5000.802 0.793 0.798 0.700
    2500.471 0.437 0.454 0.356
    1250.302 0.290 0.296 0.198
    62.50.243 0.229 0.236 0.138
    00.099 0.097 0.098 　
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human DEFB4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled DEFB4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

    显示更多

    收起更多

  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

    显示更多

    收起更多

  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Can bind to both human and mouse CCR6 and induce chemotactic activity of CCR6-expressing cells.
  • 基因功能参考文献：
    1. The encoded peptide has antimicrobial activity against E.coli, S. aureus, and P. aeruginosa. PMID: 9727055
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Beta-defensin family, LAP/TAP subfamily
  • 组织特异性：
    Expressed in the skin and respiratory tract.
  • 数据库链接：

    HGNC: 2767

    OMIM: 602215

    KEGG: hsa:100289462

    STRING: 9606.ENSP00000424598

    UniGene: Hs.105924