Human β-hexosaminidase A,β-Hex A ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Human βHex A ELISA Kit was designed for the quantitative measurement of Human βHex A protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 1.56 mIU/mL-100 mIU/mL and the sensitivity is 0.39 mIU/mL.
别名：

beta-hexosaminidase A, beta-Hex A
缩写：

βHex A
Uniprot No.：
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

1.56 mIU/mL-100 mIU/mL
灵敏度：

0.39 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
Range %	85-94
1:2	Average %	93
Range %	88-94
1:4	Average %	105
Range %	100-112
1:8	Average %	86
Range %	80-92

回收率：

The recovery of human β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	92	88-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.