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Mouse β-defensins 1 ELISA Kit

  • 中文名称：
    小鼠β-防御素1酶联免疫试剂盒
  • 货号：
    CSB-E14188m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse DEFB1 ELISA Kit was designed for the quantitative measurement of Mouse DEFB1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Defb1Beta-defensin 1 ELISA kit; BD-1 ELISA kit; mBD-1 ELISA kit; Defensin ELISA kit; beta 1 ELISA kit
  • 缩写：
    DEFB1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Microbiology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-defensins 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 93  
    Range % 89-106  
    1:10 Average % 92  
    Range % 83-100  
    1:20 Average % 95  
    Range % 85-110  
    1:40 Average % 94  
    Range % 89-103  
  • 回收率：
    The recovery of mouse β-defensins 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 91-103  
    EDTA plasma (n=4) 100 95-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.184 2.052 2.118 1.936  
    500 1.363 1.377 1.370 1.188  
    250 0.890 0.861 0.876 0.694  
    125 0.517 0.482 0.500 0.318  
    62.5 0.375 0.364 0.370 0.188  
    31.2 0.286 0.267 0.277 0.095  
    15.6 0.231 0.225 0.228 0.046  
    0 0.184 0.179 0.182    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.
  • 基因功能参考文献：
    1. Fenofibrate and gemfibrozil inhibit LPS-induced inflammatory activation of macrophages via PPARalpha/beta-defensin 1/TLR4 pathway. PMID: 25881202
    2. these studies demonstrate a role for the murine beta-defensin 1 peptide in early control of Candida infection in a murine model of mucosal candidiasis PMID: 25595775
    3. Akt1 is involved in the regulation of defensin expression and the innate immune response important for bacterial clearance PMID: 21559496
    4. Histopathology showed a greater inflammatory influx in the lungs of mBD-1((-/-)) mice at Day 3 postinfection compared with WT C57BL/6 mice PMID: 21551252
    5. The decreased production of antimicrobial peptides by burn-site epidermal keratinocytes influenced by Gr-1(+)CD11b(+) cells was shown to be restored by glycyrrhizin. PMID: 19843573
    6. elimination of mBD-1 results in a defect in the ability of the host to clear H. influenzae from the lung, which is most consistent with this peptide functioning as an antibiotic at the airway surface. PMID: 12010999
    7. Murine beta-defensins-1 and -4 were present in newborn skin. PMID: 12612195
    8. The expression of BD1 was significantly elevated in the lungs of cigarette smoke-exposed mice compared with air-exposed mice. PMID: 18699806
    9. Although both murine beta-defensin (mBD)-1 and mBD2 are constitutively expressed in normal BALB/c and C57BL/6 corneas, mBD-1 is not required for host resistance against Pseudomonas aeruginosa-induced corneal infection. PMID: 19155510

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  • 亚细胞定位：
    Secreted. Membrane.
  • 蛋白家族：
    Beta-defensin family
  • 组织特异性：
    Detected in kidney.
  • 数据库链接：