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  4. Mouse Aminopeptidase N(ANPEP) ELISA kit

Mouse Aminopeptidase N(ANPEP) ELISA kit

  • 中文名称：
    鼠氨肽酶N(ANPEP)酶联免疫试剂盒
  • 货号：
    CSB-EL001827MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse ANPEP ELISA Kit was designed for the quantitative measurement of Mouse ANPEP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39 mU/mL-2500 mU/mL and the sensitivity is 9.7 mU/mL .

  • 别名：
    Anpep ELISA kit; Lap-1 ELISA kit; Lap1Aminopeptidase N ELISA kit; AP-N ELISA kit; mAPN ELISA kit; EC 3.4.11.2 ELISA kit; Alanyl aminopeptidase ELISA kit; Aminopeptidase M ELISA kit; AP-M ELISA kit; Membrane protein p161 ELISA kit; Microsomal aminopeptidase ELISA kit; CD antigen CD13 ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39 mU/mL-2500 mU/mL
  • 灵敏度：
    9.7 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANPEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 84-97  
    1:2 Average % 95  
    Range % 90-99  
    1:4 Average % 102  
    Range % 95-106  
    1:8 Average % 95  
    Range % 89-99  
  • 回收率：
    The recovery of mouse ANPEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 82-96  
    EDTA plasma (n=4) 97 93-104  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected  
    2500 2.500 2.400 2.450 2.285  
    1250 1.970 1.830 1.900 1.735  
    625 1.119 1.075 1.097 0.932  
    312.5 0.706 0.717 0.712 0.547  
    156.25 0.447 0.458 0.453 0.288  
    78 0.304 0.315 0.310 0.145  
    39 0.246 0.252 0.249 0.084  
    0 0.164 0.165 0.165 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity.
  • 基因功能参考文献：
    1. we concluded that APN plays a significant role in the regulation of several sperm functions and early embryonic development. In addition, increased APN activity could potentially lead to several adverse consequences related to male fertility. PMID: 28859152
    2. These studies identified CD13 as a novel negative regulator of mast cell activation in vitro and in vivo. PMID: 26936360
    3. 14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID: 26208633
    4. Alanyl aminopeptidase expression is confined to mature zymogenic chief cells and its expression is lost en route to metaplasia. PMID: 26514774
    5. CD13 negatively regulates TLR4 signaling, thereby balancing the innate response by maintaining the inflammatory equilibrium critical to innate immune regulation. PMID: 25801433
    6. The impact of loss of CD13 on a model of ischemic skeletal muscle injury, was investigated. PMID: 24307555
    7. Molecular mechanisms regulating CD13-mediated adhesion. PMID: 24627994
    8. CD13 is essential for proper trafficking of the inflammatory cells necessary to prime and sustain the reparative response, thus promoting optimal post-infarction healing. PMID: 23761403
    9. Tyrosine phosphorylation of CD13 regulates inflammatory cell-cell adhesion and monocyte trafficking. PMID: 23997214
    10. This indicates that Anpep plays a critical role in the proteolytic remodelling of mammary tissue during adult mammary development. PMID: 22983824
    11. the presence of digestive protein complexes in the intestinal brush-border containing the peptidases APN and ACE2 and the neutral amino acid transporter B0AT1 PMID: 22677001
    12. cooperation in APN expression by both cancer cells and nonmalignant stromal cells within the tumor microenvironment promotes angiogenesis, tumor growth, and metastasis PMID: 22307623
    13. expression of CD13 in normal and pathological human tissues PMID: 21339174
    14. Data show that the majority of chondrocyte-like cells are of bone marrow origin, whereas CD34(+)/CD13(+) myeloid precursors appear to infiltrate the plaque actively and transdifferentiate into chondrocyte-like cells in the progression of atherosclerosis. PMID: 20489139
    15. although CD13 is highly expressed on myeloid cells and is a reliable marker of the myeloid lineage of normal and leukemic cells, it is not a critical regulator of hematopoietic development, hemostasis, or myeloid cell function PMID: 20430777
    16. CD13 expressed in microglia-derived exosomes is active in neuropeptide degradation and could have a distinct effect on local neuropeptide activity in the brain. PMID: 16081791
    17. Results suggest that CD26 and CD13 play a role in T cell function in tissue-specific autoimmunity in the central nervous system. PMID: 16700521
    18. In cardiac angiogenesis endothelial CD13/APN is upregulated. It can be targeted specifically with cNGR conjugates. In the heart cNGR binds its endothelial target only in angiogenic areas. PMID: 16990557
    19. aminopeptidase N has a role in angiogenesis PMID: 17360568

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Peptidase M1 family
  • 组织特异性：
    Expressed in the intestinal brush border (at protein level). Highly expressed in intestinal tract and kidney, present in liver, lymph node, spleen, and brain. Found as well in monocytes, macrophages, dendritic cells, veiled cells and B-cells but not on T-
  • 数据库链接：

    KEGG: mmu:16790

    STRING: 10090.ENSMUSP00000035943

    UniGene: Mm.4487