Mouse Angiopoietin 1,ANG-1 ELISA Kit

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

1. hyperoxia as well as increased TGFbeta signaling is a major contributor to the increased levels of miR34a, which mediate injury via angiopoietin-1 in neonatal lungs PMID: 29079808
2. Data (including data from studies in transgenic mice) suggest that Dkk2 secreted from pericytes promotes angiogenesis and neurogenesis/neural regeneration via Angpt1/Tie2/Wnt signaling; here, overexpression of Dkk2 in diabetic mice enhances penile angiogenesis/neural regeneration and restores erectile function. (Dkk2 = dickkopf-2; Angpt1= angiopoietin-1; Tie2 = endothelial-specific receptor tyrosine kinase Tie2) PMID: 29559443
3. Angiopoietin-1/Tek vascular growth factor system leads to increased metastasis without affecting primary tumor growth. PMID: 28800750
4. Mesenchymal stem cell microvesicles attenuate acute lung injury in mice at least partially via angiopopietin-1 mRNA. PMID: 28376568
5. results suggest that loss of Angpt1 is central in capillary rarefaction and fibrogenesis and propose that manipulations to maintain Angpt1 levels may slow down fibrosis progression PMID: 29293543
6. These data define a role for dysregulation of the Ang-Tie-2 axis in the pathogenesis of cerebral malaria. PMID: 27683553
7. These results indicate that the Angpt-Tie2 system is essential for SC integrity. The impairment of this system underlies POAG-associated pathogenesis, supporting the possibility that Tie2 agonists could be a therapeutic option for glaucoma. PMID: 28920924
8. we demonstrate that targeted Ang-1 overexpression attenuates myofibroblast activation and interstitial collagen I accumulation, inhibits the upregulation of transforming growth factor beta1 and subsequent phosphorylation of Smad 2/3, dampens renal inflammation, and stimulates the growth of peritubular capillaries in the obstructed kidney. PMID: 27454431
9. Hydroxysafflor yellow A promotes angiogenesis in ischemic hindlimb via the angiopoietin 1/ Tie-2 signaling pathway. PMID: 27894114
10. COMP-Ang1 can enhance BMP2-induced cranial bone regeneration with increased pericyte recruitment. Combined delivery of the proteins might be a therapeutic strategy to repair cranial bone damage. PMID: 26465321
11. Hematopoietic stem cells regulate the regeneration of their niche by secreting Angpt1. PMID: 25821987
12. Myocardium-derived angiopoietin-1 is essential for coronary vein formation in the developing heart. PMID: 25072663
13. Ang-1 strongly enhances skeletal muscle regeneration in response to fiber injury and that this effect is mediated through induction of the myogenesis program in muscle progenitor cells and the angiogenesis program in endothelial cells. PMID: 25608750
14. These results suggest that PRP extract prevents endotoxin-induced pulmonary edema mainly through Ang-Tie2 signaling, and PRP extract could be a potential therapeutic strategy for sepsis-induced pulmonary edema PMID: 24960457
15. E2 increases Ang-1 and enhances capillary density in brain under basal conditions through estrogen receptor-alpha, priming the middle cerebral artery territory for survival after experimental focal ischemia PMID: 15637314
16. The important role of Ang-1 in the activation of mast cells, and a potential therapeutic effect of Ang-1 on anaphylaxis diseases. PMID: 24586553
17. findings report that Ang-1 induces arteriolar vasodilation via release of NO, suggesting that Ang-1 is an important regulator of microvascular tone. PMID: 24407281
18. Sonic hedgehog secreted by neurons regulates ang-1 and ang-2 expression in neighboring fibroblasts. PMID: 24807580
19. The Ang-1, Ang-2, Tie-2 system takes part in the pathologic processes in the brains that suffered acute carbon monoxide poisoning by participating in neovascularization. PMID: 23424209
20. Genetic deletion of Ang1 exacerbated, while overexpression of Ang1 suppressed CNV formation and vascular leakage. PMID: 24609620
21. Podocyte-specific inducible repletion of angiopoietin-1 in diabetic mice caused a 70% reduction of albuminuria and prevented diabetes-induced glomerular endothelial cell proliferation; hyperfiltration and renal morphology were unchanged. PMID: 24009238
22. Haptoglobin deficiency is associated with leakage-prone vessels via isolated VEGFA induction and/or suppressed Ang-1 expression, resulting in unbalanced VEGFalpha/Ang-1 transcription. PMID: 23274064
23. ligand-independent dimerization of Tie2 is essential for a strong response upon stimulation with high dose Ang1. PMID: 23504320
24. Vascular endothelial growth factor and Angiopoietin-1 were shown to play important roles in recovery from renal injury, particularly in the regeneration of endothelial injury. PMID: 23171542
25. Ang-1 appears important in normal lung development, and we have established its tissue-level expression and localization patterns at key time-points. PMID: 22917924
26. These data suggest that Angpt1-Tie2 signaling is crucial for the regulation of hematopoietic lineage differentiation and the function of LT-HSCs. PMID: 23149415
27. LRP5-Tie2-Ang signaling axis plays a central role in control of both angiogenesis and alveolarization during postnatal lung development PMID: 22848540
28. These results provide an insight into the molecular mechanisms by which Ang1 directly affects the myogenesis of striated muscle. PMID: 23041942
29. Ang-1 attenuates cardiac apoptosis and promotes cardiac repair by a mechanism involving in promoting CD133(+)/c-kit(+) cells and angiogenesis in diabetic db/db mouse infarcted hearts. PMID: 22558265
30. Decreased angiopoietin1/Tie2 and increased angiopoietin2 expression may contribute to diabetes-induced vascular damage after stroke in type-2 diabetic mice. PMID: 21515377
31. Dopamine stabilizes tumor blood vessels by up-regulating angiopoietin 1 expression in pericytes and Kruppel-like factor-2 expression in tumor endothelial cells PMID: 22143796
32. Unique angiogenic and vasculogenic properties of renal cell carcinoma in a xenograft model of bone metastasis are associated with high levels of vegf-a and decreased ang-1 expression. PMID: 21809376
33. coexpression of ANG1 with Vascular endothelial growth factor improved blood-brain barrier integrity and resulted in better neuroprotection PMID: 21772310
34. The findings also show that the antileakage action of Ang1 requires PDGF-dependent actions of pericytes in maintaining endothelial stability. PMID: 21550017
35. ANG-1 is critical for regulating the number &diameter of developing vessels. It is not required for pericyte recruitment. It is dispensable in quiescent vessels but powerfully modulates the vascular response after injury. PMID: 21606590
36. Ang-1 treatment downregulates pulmonary inflammation, VEGF and Ang-2 expression but does not protect against vascular leakage and impaired gas exchange induced by HVT-ventilation PMID: 21179479
37. Ang-1 attenuated high glucose-induced endothelial cell dysfunction in mouse heart myocardial endothelial cells. PMID: 21113176
38. increased expression of Tie2 and Ang2 in allergic airway epithelium and alveolar macrophages, but not Ang1 or VEGF correlates with the severity of airway remodeling PMID: 20463289
39. Angiopoietin/Tie2 signaling transforms capillaries into venules primed for leukocyte trafficking in airway inflammation PMID: 20133818
40. The role of Ang-1 on SVZ neurogenesis (proliferation, differentiation, and axonogenesis)is unraveled. PMID: 20357108
41. Data suggest that inhibition of Angiopoietin 2 leads to normalization of tumor blood vessels by permitting the unopposed action of Ang1, but decreases tumor vascularity primarily by blocking Ang2 actions. PMID: 19815705
42. can offset VEGF-induced angiogenesis PMID: 12048246
43. in hypoplastic lungs, increased ANG-1 protein may be required to maintain vessel integrity and quiescence PMID: 12060561
44. Ang1 reduces blood-brain barrier leakage in ischemic brain and consequently decreases ischemic lesion volume. PMID: 12150788
45. Angiopoietin-1 can genetically rescue defective lymphatic vessels but not angiogenesis defects in knockout mice. PMID: 12361603
46. determination of binding sites in tie-2 receptor PMID: 12427764
47. Genetic engineering of precise multimers of the receptor-binding domain of angiopoietin-1 reveals that tetramers are the minimal size required for activating endothelial Tie2 receptors. PMID: 12469114
48. results indicated that skin tumor development occurred in a strong angiogenesis-prone scenario in which PlGF and Ang-2 acted cooperatively with VEGF, whereas the negative or stabilizing effect of Ang-1 was abrogated PMID: 12766907
49. Selective functions of angiopoietins and vascular endothelial growth factor on blood vessels: the concept of "vascular domain". PMID: 12858538
50. Complementary and coordinated roles of the VEGFs and angiopoietins during normal and pathologic vascular formation. PMID: 12858549

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Secreted.

KEGG: mmu:11600

STRING: 10090.ENSMUSP00000022921

UniGene: Mm.309336