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  4. Recombinant Mouse Angiopoietin-1 (Angpt1)

Recombinant Mouse Angiopoietin-1 (Angpt1)

  • 货号：
    CSB-YP001706MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001706MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001706MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001706MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001706MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Angpt1; AgptAngiopoietin-1; ANG-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-498
  • 氨基酸序列
    S NQRRNPENGG RRYNRIQHGQ CAYTFILPEH DGNCRESATE QYNTNALQRD APHVEPDFSS QKLQHLEHVM ENYTQWLQKL ENYIVENMKS EMAQIQQNAV QNHTATMLEI GTSLLSQTAE QTRKLTDVET QVLNQTSRLE IQLLENSLST YKLEKQLLQQ TNEILKIHEK NSLLEHKILE MEGKHKEELD TLKEEKENLQ GLVSRQTFII QELEKQLSRA TNNNSILQKQ QLELMDTVHN LISLCTKEGV LLKGGKREEE KPFRDCADVY QAGFNKSGIY TIYFNNMPEP KKVFCNMDVN GGGWTVIQHR EDGSLDFQRG WKEYKMGFGN PSGEYWLGNE FIFAITSQRQ YMLRIELMDW EGNRAYSQYD RFHIGNEKQN YRLYLKGHTG TAGKQSSLIL HGADFSTKDA DNDNCMCKCA LMLTGGWWFD ACGPSNLNGM FYTAGQNHGK LNGIKWHYFK GPSYSLRSTT MMIRPLDF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
  • 基因功能参考文献：
    1. hyperoxia as well as increased TGFbeta signaling is a major contributor to the increased levels of miR34a, which mediate injury via angiopoietin-1 in neonatal lungs PMID: 29079808
    2. Data (including data from studies in transgenic mice) suggest that Dkk2 secreted from pericytes promotes angiogenesis and neurogenesis/neural regeneration via Angpt1/Tie2/Wnt signaling; here, overexpression of Dkk2 in diabetic mice enhances penile angiogenesis/neural regeneration and restores erectile function. (Dkk2 = dickkopf-2; Angpt1= angiopoietin-1; Tie2 = endothelial-specific receptor tyrosine kinase Tie2) PMID: 29559443
    3. Angiopoietin-1/Tek vascular growth factor system leads to increased metastasis without affecting primary tumor growth. PMID: 28800750
    4. Mesenchymal stem cell microvesicles attenuate acute lung injury in mice at least partially via angiopopietin-1 mRNA. PMID: 28376568
    5. results suggest that loss of Angpt1 is central in capillary rarefaction and fibrogenesis and propose that manipulations to maintain Angpt1 levels may slow down fibrosis progression PMID: 29293543
    6. These data define a role for dysregulation of the Ang-Tie-2 axis in the pathogenesis of cerebral malaria. PMID: 27683553
    7. These results indicate that the Angpt-Tie2 system is essential for SC integrity. The impairment of this system underlies POAG-associated pathogenesis, supporting the possibility that Tie2 agonists could be a therapeutic option for glaucoma. PMID: 28920924
    8. we demonstrate that targeted Ang-1 overexpression attenuates myofibroblast activation and interstitial collagen I accumulation, inhibits the upregulation of transforming growth factor beta1 and subsequent phosphorylation of Smad 2/3, dampens renal inflammation, and stimulates the growth of peritubular capillaries in the obstructed kidney. PMID: 27454431
    9. Hydroxysafflor yellow A promotes angiogenesis in ischemic hindlimb via the angiopoietin 1/ Tie-2 signaling pathway. PMID: 27894114
    10. COMP-Ang1 can enhance BMP2-induced cranial bone regeneration with increased pericyte recruitment. Combined delivery of the proteins might be a therapeutic strategy to repair cranial bone damage. PMID: 26465321
    11. Hematopoietic stem cells regulate the regeneration of their niche by secreting Angpt1. PMID: 25821987
    12. Myocardium-derived angiopoietin-1 is essential for coronary vein formation in the developing heart. PMID: 25072663
    13. Ang-1 strongly enhances skeletal muscle regeneration in response to fiber injury and that this effect is mediated through induction of the myogenesis program in muscle progenitor cells and the angiogenesis program in endothelial cells. PMID: 25608750
    14. These results suggest that PRP extract prevents endotoxin-induced pulmonary edema mainly through Ang-Tie2 signaling, and PRP extract could be a potential therapeutic strategy for sepsis-induced pulmonary edema PMID: 24960457
    15. E2 increases Ang-1 and enhances capillary density in brain under basal conditions through estrogen receptor-alpha, priming the middle cerebral artery territory for survival after experimental focal ischemia PMID: 15637314
    16. The important role of Ang-1 in the activation of mast cells, and a potential therapeutic effect of Ang-1 on anaphylaxis diseases. PMID: 24586553
    17. findings report that Ang-1 induces arteriolar vasodilation via release of NO, suggesting that Ang-1 is an important regulator of microvascular tone. PMID: 24407281
    18. Sonic hedgehog secreted by neurons regulates ang-1 and ang-2 expression in neighboring fibroblasts. PMID: 24807580
    19. The Ang-1, Ang-2, Tie-2 system takes part in the pathologic processes in the brains that suffered acute carbon monoxide poisoning by participating in neovascularization. PMID: 23424209
    20. Genetic deletion of Ang1 exacerbated, while overexpression of Ang1 suppressed CNV formation and vascular leakage. PMID: 24609620
    21. Podocyte-specific inducible repletion of angiopoietin-1 in diabetic mice caused a 70% reduction of albuminuria and prevented diabetes-induced glomerular endothelial cell proliferation; hyperfiltration and renal morphology were unchanged. PMID: 24009238
    22. Haptoglobin deficiency is associated with leakage-prone vessels via isolated VEGFA induction and/or suppressed Ang-1 expression, resulting in unbalanced VEGFalpha/Ang-1 transcription. PMID: 23274064
    23. ligand-independent dimerization of Tie2 is essential for a strong response upon stimulation with high dose Ang1. PMID: 23504320
    24. Vascular endothelial growth factor and Angiopoietin-1 were shown to play important roles in recovery from renal injury, particularly in the regeneration of endothelial injury. PMID: 23171542
    25. Ang-1 appears important in normal lung development, and we have established its tissue-level expression and localization patterns at key time-points. PMID: 22917924
    26. These data suggest that Angpt1-Tie2 signaling is crucial for the regulation of hematopoietic lineage differentiation and the function of LT-HSCs. PMID: 23149415
    27. LRP5-Tie2-Ang signaling axis plays a central role in control of both angiogenesis and alveolarization during postnatal lung development PMID: 22848540
    28. These results provide an insight into the molecular mechanisms by which Ang1 directly affects the myogenesis of striated muscle. PMID: 23041942
    29. Ang-1 attenuates cardiac apoptosis and promotes cardiac repair by a mechanism involving in promoting CD133(+)/c-kit(+) cells and angiogenesis in diabetic db/db mouse infarcted hearts. PMID: 22558265
    30. Decreased angiopoietin1/Tie2 and increased angiopoietin2 expression may contribute to diabetes-induced vascular damage after stroke in type-2 diabetic mice. PMID: 21515377
    31. Dopamine stabilizes tumor blood vessels by up-regulating angiopoietin 1 expression in pericytes and Kruppel-like factor-2 expression in tumor endothelial cells PMID: 22143796
    32. Unique angiogenic and vasculogenic properties of renal cell carcinoma in a xenograft model of bone metastasis are associated with high levels of vegf-a and decreased ang-1 expression. PMID: 21809376
    33. coexpression of ANG1 with Vascular endothelial growth factor improved blood-brain barrier integrity and resulted in better neuroprotection PMID: 21772310
    34. The findings also show that the antileakage action of Ang1 requires PDGF-dependent actions of pericytes in maintaining endothelial stability. PMID: 21550017
    35. ANG-1 is critical for regulating the number &diameter of developing vessels. It is not required for pericyte recruitment. It is dispensable in quiescent vessels but powerfully modulates the vascular response after injury. PMID: 21606590
    36. Ang-1 treatment downregulates pulmonary inflammation, VEGF and Ang-2 expression but does not protect against vascular leakage and impaired gas exchange induced by HVT-ventilation PMID: 21179479
    37. Ang-1 attenuated high glucose-induced endothelial cell dysfunction in mouse heart myocardial endothelial cells. PMID: 21113176
    38. increased expression of Tie2 and Ang2 in allergic airway epithelium and alveolar macrophages, but not Ang1 or VEGF correlates with the severity of airway remodeling PMID: 20463289
    39. Angiopoietin/Tie2 signaling transforms capillaries into venules primed for leukocyte trafficking in airway inflammation PMID: 20133818
    40. The role of Ang-1 on SVZ neurogenesis (proliferation, differentiation, and axonogenesis)is unraveled. PMID: 20357108
    41. Data suggest that inhibition of Angiopoietin 2 leads to normalization of tumor blood vessels by permitting the unopposed action of Ang1, but decreases tumor vascularity primarily by blocking Ang2 actions. PMID: 19815705
    42. can offset VEGF-induced angiogenesis PMID: 12048246
    43. in hypoplastic lungs, increased ANG-1 protein may be required to maintain vessel integrity and quiescence PMID: 12060561
    44. Ang1 reduces blood-brain barrier leakage in ischemic brain and consequently decreases ischemic lesion volume. PMID: 12150788
    45. Angiopoietin-1 can genetically rescue defective lymphatic vessels but not angiogenesis defects in knockout mice. PMID: 12361603
    46. determination of binding sites in tie-2 receptor PMID: 12427764
    47. Genetic engineering of precise multimers of the receptor-binding domain of angiopoietin-1 reveals that tetramers are the minimal size required for activating endothelial Tie2 receptors. PMID: 12469114
    48. results indicated that skin tumor development occurred in a strong angiogenesis-prone scenario in which PlGF and Ang-2 acted cooperatively with VEGF, whereas the negative or stabilizing effect of Ang-1 was abrogated PMID: 12766907
    49. Selective functions of angiopoietins and vascular endothelial growth factor on blood vessels: the concept of "vascular domain". PMID: 12858538
    50. Complementary and coordinated roles of the VEGFs and angiopoietins during normal and pathologic vascular formation. PMID: 12858549

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    KEGG: mmu:11600

    STRING: 10090.ENSMUSP00000022921

    UniGene: Mm.309336