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  4. Mouse Angiopoietin-related protein 6(ANGPTL6) ELISA kit

Mouse Angiopoietin-related protein 6(ANGPTL6) ELISA kit

  • 中文名称：
    小鼠血管生成素相关蛋白6(ANGPTL6)酶联免疫试剂盒
  • 货号：
    CSB-EL001714MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse ANGPTL6 ELISA Kit was designed for the quantitative measurement of Mouse ANGPTL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    Angptl6 ELISA kit; AgfAngiopoietin-related protein 6 ELISA kit; Angiopoietin-like protein 6 ELISA kit; Angiopoietin-related growth factor ELISA kit
  • 缩写：
    ANGPTL6
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %89
    Range %82-96
    1:200Average %98
    Range %93-102
    1:400Average %99
    Range %95-107
    1:800Average %91
    Range %87-95
  • 回收率：
    The recovery of mouse ANGPTL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9184-95
    EDTA plasma (n=4)9996-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.616 2.793 2.705 2.546
    20001.927 2.036 1.982 1.823
    10001.287 1.388 1.338 1.179
    5000.769 0.729 0.749 0.590
    2500.577 0.604 0.591 0.432
    1250.394 0.401 0.398 0.239
    62.50.247 0.267 0.257 0.098
    00.164 0.154 0.159 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    May play a role in the wound healing process. May promote epidermal proliferation, remodeling and regeneration. May promote the chemotactic activity of endothelial cells and induce neovascularization. May counteract high-fat diet-induced obesity and related insulin resistance through increased energy expenditure.
  • 基因功能参考文献：
    1. Since upregulation of ANGPTL6 is induced on metabolic stress, we investigated the hepatic expression of ANGPTL6 by leptin, a representative adipokine of obesity. Mice on a high-fat diet showed increased serum leptin levels and hepatic Angptl6 expression. PMID: 29852166
    2. Upregulation of ANGPTL6 in mouse keratinocytes enhances susceptibility to psoriasis. PMID: 27698489
    3. mitochondrial OXPHOS function governs the expression of ANGPTL6, which is an essential factor for FGF21 production in adipose tissue and cultured adipocytes. PMID: 28184000
    4. In addition to pronounced angiogenesis and epidermal hyperplasia, marked enlargement of dermal lymphatic vessels was observed in UV-B-exposed Angptl6/AGF transgenic mice. PMID: 22132739
    5. we show that (>80%) of the AGF-deficient mice die at about embryonic day 13, while the surviving AGF-deficient mice develop marked obesity, lipid accumulation in skeletal muscle and liver, and insulin resistance accompanied by reduced energy expenditure PMID: 15778720
    6. Our results strongly suggest that AGF is an integrin ligand as well as a mitogenic growth factor and theoretically participates in cutaneous wound healing in a more complex mechanism. PMID: 16806062
    7. Activation of an ERK1/2-eNOS-NO pathway is a crucial signaling mechanism by which angiopoietin-related growth factor increases blood flow through induction of angiogenesis and arteriogenesis. PMID: 18258819

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Highly expressed in the liver, specifically in hepatocytes, and weakly in the heart. Expressed in hematopoietic cells, platelets and mast cells, and detected at wounded skin.
  • 数据库链接：