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Mouse Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit

  • 中文名称:
    小鼠Ⅰ型前胶原羧基端肽(PⅠCP)酶联免疫试剂盒
  • 货号:
    CSB-E08080m
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Mouse PⅠCP ELISA Kit was designed for the quantitative measurement of Mouse PⅠCP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

  • 别名:
    Carboxyterminal propeptide of type 1 procollagen,P1CP,_x000D_ Carboxyterminal propeptide of type I procollagen,PICP
  • 缩写:
    PⅠCP
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma
  • 检测范围:
    31.25 pg/mL-2000 pg/mL
  • 灵敏度:
    7.81 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 90-99  
    1:200 Average % 91  
    Range % 85-96  
    1:400 Average % 87  
    Range % 82-93  
    1:800 Average % 96  
    Range % 92-102  
  • 回收率:
    The recovery of mouse PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 86-95  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.821 2.731 2.776 2.630  
    1000 2.132 2.314 2.223 2.077  
    500 1.749 1.673 1.711 1.565  
    250 1.097 1.150 1.124 0.978  
    125 0.695 0.646 0.671 0.525  
    62.5 0.407 0.387 0.397 0.251  
    31.25 0.332 0.317 0.325 0.179  
    0 0.148 0.144 0.146    
  • 数据处理:
  • 货期:
    3-5 working days

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