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  4. Mouse Cardiotrophin-like cytokine factor 1(CLCF1) ELISA kit

Mouse Cardiotrophin-like cytokine factor 1(CLCF1) ELISA kit

  • 中文名称：
    小鼠心脏营养素样细胞因子1(CLCF1)酶联免疫试剂盒
  • 货号：
    CSB-EL005479MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse CLCF1 ELISA Kit was designed for the quantitative measurement of Mouse CLCF1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    Clcf1 ELISA kit; Bsf3 ELISA kit; Nnt1Cardiotrophin-like cytokine factor 1 ELISA kit; B-cell-stimulating factor 3 ELISA kit; BSF-3 ELISA kit; Novel neurotrophin-1 ELISA kit; NNT-1 ELISA kit
  • 缩写：
    CLCF1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLCF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-94
    1:2 Average % 89
    Range % 85-93
    1:4 Average % 103
    Range % 98-109
    1:8 Average % 85
    Range % 80-90
  • 回收率：
    The recovery of mouse CLCF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-100
    EDTA plasma (n=4) 91 85-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.401 2.293 2.347 2.204
    750 1.927 1.876 1.902 1.759
    375 1.315 1.361 1.338 1.195
    187.5 0.869 0.837 0.853 0.710
    94 0.574 0.584 0.579 0.436
    47 0.338 0.319 0.329 0.186
    23.5 0.232 0.245 0.239 0.096
    0 0.146 0.140 0.143 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development. Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.
  • 基因功能参考文献：
    1. Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders. PMID: 26198769
    2. CLC plays a role in astrocyte differentiation via STAT3 activation within the developing brain PMID: 12090754
    3. IL-6 (gp130) family cytokine members include CLC/NNT1/BSF3 and IL-27 [review] PMID: 15253382
    4. In this study, Clc knockout mice had decreased facial motility, did not suckle, died within 24 hours, and had 32% and 29% fewer motor neurons in the facial nucleus and lumbar ventral horn, respectively. PMID: 19098279
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 数据库链接：