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  4. Mouse Ceruloplasmin,CP/CER ELISA Kit

Mouse Ceruloplasmin,CP/CER ELISA Kit

  • 中文名称：
    小鼠铜蓝蛋白(CP/CER)酶联免疫试剂盒
  • 货号：
    CSB-E07023m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse Ceruloplasmin (CP) ELISA Kit was designed for the quantitative measurement of Mouse Ceruloplasmin (CP) protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.2 ng/mL-2000 ng/mL and the sensitivity is 7.8 ng/mL.

  • 别名：
    Cp ELISA Kit; Ceruloplasmin ELISA Kit; EC 1.16.3.1 ELISA Kit; Ferroxidase ELISA Kit
  • 缩写：
    CP
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    31.2 ng/mL-2000 ng/mL
  • 灵敏度：
    7.8 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CP/CER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 95  
    Range % 83-106  
    1:2000 Average % 92  
    Range % 83-104  
    1:4000 Average % 95  
    Range % 91-102  
    1:8000 Average % 93  
    Range % 87-99  
  • 回收率：
    The recovery of mouse CP/CER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-99  
    EDTA plasma (n=4) 92 85-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2000 0.103 0.105 0.104    
    1000 0.169 0.171 0.170    
    500 0.301 0.309 0.305    
    250 0.575 0.582 0.579    
    125 1.294 1.306 1.300    
    62.5 1.855 1.864 1.860    
    31.2 2.536 2.541 2.539    
    0 2.879 2.887 2.883    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense.
  • 基因功能参考文献：
    1. We thus used hephaestin (Heph) and ceruloplasmin (Cp) single-knockout mice and Heph/Cp double-knockout mice to investigate the roles of multicopper ferroxidases(MCF) in pancreatic iron homeostasis. We found that both HEPH and CP were expressed in the mouse pancreas, and that ablation of either MCF had limited effect on the pancreatic iron levels. PMID: 29883959
    2. Hephaestin and ceruloplasmin have roles in facilitating iron metabolism in the mouse kidney PMID: 27991585
    3. In D-galactosamine-sensitized mice CP+Cu(II) increased the LPS-induced lethality from 54 to 100%, while administration of antibodies against MIF prevented the lethal effect. The enhancement by CP+Cu(II) of the pro-inflammatory signal of MIF is discussed PMID: 26091949
    4. mice with mutation of Cp and Heph, iron accumulates in glia, while neurons have low iron levels. Both neurons and glia degenerate and mice become ataxic unless given an iron chelator. PMID: 26303407
    5. Data (including data from studies in knockout mice) suggest that ceruloplasmin and hephaestin play distinct roles in regulation of gene expression in various regions of the brain and are involved in iron homeostasis. PMID: 25788583
    6. an increase in Cp expression could contribute to tissue damage in early experimental autoimmune encephalomyelitis PMID: 24615902
    7. Evidence supports a regulatory role of both proteins (Ceruloplasmin (CP) and beta-amyloid protein precursor (APP)) in defence against iron-induced oxidative damage after TBI, which presents as a tractable therapeutic target. PMID: 24509156
    8. Genetic interactions between Cp, Mon1a, and the Slc40a1 locus are involved in iron metabolism. PMID: 24121729
    9. ceruloplasmin should provide a protective shield against inadvertent oxidant production by myeloperoxidase during inflammation PMID: 23306200
    10. The mouse ceruloplasmin gene has been mapped to chromosome 3. PMID: 178338
    11. Data found an increase in ceruloplasmin levels in the plasma of Npc1 -/- mice compared to Npc1 +/+ mice, and this increase was statistically significant (*p < 0.05). PMID: 22526561
    12. Cp and Heph are necessary for iron export from the retina but are not essential for iron import into the retina. PMID: 22342521
    13. levels of 5HT and norepinephrine and the expression of BDNF and its receptor trkB, are significantly reduced in the hippocampus of ceruloplasmin knockout mice PMID: 22035068
    14. Ceruloplasmin deficiency reduces levels of iron and BDNF in the cortex and striatum of young mice and increases their vulnerability to stroke. PMID: 21949858
    15. Ceruloplasmin stimulation of reelin cleavage is in line with a possible role of ceruloplasmin in nervous system development. PMID: 20188154
    16. Reduction in Cp is a sensitive biomarker for copper deficiency. PMID: 20170749
    17. mainly acts to release iron from cells in the liver PMID: 12393173
    18. Glycosylphosphatidylinositol-anchored ceruloplasmin is required for iron efflux from cells in the central nervous system. PMID: 12743117
    19. Cp has a pro-aggregative action on newly differentiated neurons in vitro specific for Cp in a concentration-dependent and saturable fashion. PMID: 12946701
    20. The copper-containing yeast protein Fet3p can restore iron homeostasis in phlebotomized mice with a deletion of the ceruloplasmin gene. PMID: 14739215
    21. Ceruloplasmin ane hephaestin are critical for CNS iron homeostasis and that loss of Cp and Heph in the mouse leads to age-dependent retinal neurodegeneration. PMID: 15365174
    22. Iron accumulation is reflected in increased ferritin expression in ceruloplasmin deficient mice and is accompanied by cell loss. PMID: 16988052
    23. the concentrations of ceruloplasmin and lactoferrin in milk were increased fivefold and more than 38-fold, respectively, within 48 h after weaning PMID: 16989572
    24. Transcripts of ceruloplasmin, involved in iron efflux, were overexpressed in periportal areas and the result was confirmed by in situ hybridization study. PMID: 18222182
    25. ROS affects RNA-protein complex formation to promote Cp mRNA decay. PMID: 19019832
    26. Datan confirmed that mouse ceruloplasmin had ferroxidase activity but revealed an additional ferroxidase in ceruloplasmin knockout mouse plasma. PMID: 19076073
    27. Microglial and endothelial induction of Cp is a neuroprotective mechanism during inflammation because Cp-deficient mice exhibited increased iron accumulation and demyelination when exposed to LPS and neurovascular reactivity to pneumococcal meningitis PMID: 17375196
    28. To elucidate the role of ceruloplasmin in copper metabolism, the kinetics of copper absorption, transport, distribution, and excretion were examined utilizing (64)Cu in wild-type and aceruloplasminemic mice PMID: 11461924

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Multicopper oxidase family
  • 组织特异性：
    Expressed in many tissues, including liver, eye and brain.
  • 数据库链接：