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  4. Mouse Chitinase-3-like protein 1(CHI3L1) ELISA kit

Mouse Chitinase-3-like protein 1(CHI3L1) ELISA kit

  • 中文名称：
    小鼠几丁质酶3样蛋白1(CHI3L1)酶联免疫试剂盒
  • 货号：
    CSB-EL005346MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse CHI3L1 ELISA Kit was designed for the quantitative measurement of Mouse CHI3L1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.47 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL.

  • 别名：
    Chi3l1 ELISA Kit; Brp39 ELISA Kit; Chil1 ELISA Kit; Chitinase-3-like protein 1 ELISA Kit; BRP39 protein ELISA Kit; Breast regression protein 39 ELISA Kit; Cartilage glycoprotein 39 ELISA Kit; CGP-39 ELISA Kit; GP-39 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.47 ng/mL-30 ng/mL
  • 灵敏度：
    0.11 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CHI3L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 84
    Range % 80-88
    1:2 Average % 91
    Range % 86-95
    1:4 Average % 103
    Range % 99-107
    1:8 Average % 94
    Range % 85-98
  • 回收率：
    The recovery of mouse CHI3L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 85-93
    EDTA plasma (n=4) 95 90-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    30 2.601 2.476 2.539 2.319
    15 1.933 1.819 1.876 1.656
    7.5 1.140 1.204 1.172 0.952
    3.75 0.692 0.682 0.687 0.467
    1.88 0.457 0.441 0.449 0.229
    0.94 0.349 0.358 0.354 0.134
    0.47 0.284 0.297 0.291 0.071
    0 0.224 0.215 0.220 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
  • 基因功能参考文献：
    1. Chi3l1 is a regulator of Th1 and CTL which could be a therapeutic target to enhance anti-tumor immunity. PMID: 29403003
    2. Nrf2/Keap1 system regulates VSMC apoptosis during neointimal formation, thereby inhibiting neointimal hyperplasia after a vascular injury. PMID: 27198666
    3. Knockdown of Chi3l1 in the arterial endothelium suppressed the development of atherosclerosis in amyloid precursor protein transgenic mice. PMID: 29344304
    4. CHI3L1 may be a previously unrecognized biomarker for diagnosing Gaucher Diseases (GD) and for evaluating the therapeutic effects of new GD drug. PMID: 29396296
    5. CHI3L1 deficiency in donor splenocytes dramatically increased the severity of aGVHD through enhancing Tfh cell differentiation PMID: 28656528
    6. miR-24 serves as a molecular regulator in S. aureus-induced macrophage polarization through targeting of CHI3L1 and regulation of the MAPK pathway PMID: 28303416
    7. The mouse model was used to validate the function of CHI3L1 in cancer metastasis in vivo PMID: 28143526
    8. CHI3L1-mediated cell proliferation/survival involves partial downregulation of the pro-apoptotic S100A9 protein that is highly expressed during the acute phase of colitis, by binding to the S100A9 receptor, RAGE (Receptor for Advanced Glycation End products). PMID: 26431492
    9. IL-18 is a potent stimulator of Chi3l1 and that Chi3l1 is an important mediator of IL-18-induced inflammatory, fibrotic, alveolar remodeling, and cytotoxic responses. PMID: 25955511
    10. CHI3L1 is induced during fungal infection, and it regulated antifungal innate immune responses in the cornea. PMID: 26238714
    11. Astrocytic expression of chitinase 3-like protein 1 limits the extent of both astrocytic and microglial/macrophage facets of neuroinflammation. PMID: 25377763
    12. Results do not support a role of BRP-39 in the pathogenesis of experimental autoimmune encephalitis. PMID: 26079949
    13. CHI3L1 was increased in plasma and brain tissue in experimental cerebral malaria, but targeted Chi3l1 deletion did not alter cytokine production or survival in this model. PMID: 25047113
    14. mechanism of BRP-39 in bleomycin-induced pulmonary fibrosis PMID: 24659093
    15. Sem7a and its receptors regulate Chi3l1 in pulmonary melanoma metastasis PMID: 25511377
    16. Data show that IL-1-induced transcriptin factor RelB/NF-kappa B p50 Subunit complex formation was further promoted by oncostatin M and that these complexes directly bound to the secreted protein cartilage glycoprotein 39 (YKL-40) promoter. PMID: 25681350
    17. These results indicate that catalytically inactive BRP-39 and Ym1 are constitutive genes in normal mouse lung. PMID: 25294623
    18. BRP-39 is involved in repressing the M1 proinflammatory phenotype in hyperoxia, thereby deactivating inflammatory responses in macrophages and preventing neonatal lung injury. PMID: 24347826
    19. Chi3l1 binds to IL-13Ralpha2, stimulates MAPK, Akt/PKB, Wnt signaling and regulates melanoma metastasis. PMID: 23972995
    20. Stat3 downstream gene product chitinase 3-like 1 is elevated in lung cancer PMID: 23613996
    21. We identified the macrophage-secreted chitinase-like protein Brp-39 as a critical component of this reparative response that serves to limit tubular cell apoptotic death via activation of Akt, improving animal survival after kidney ischemia/reperfusion. PMID: 23291472
    22. BRP-39-deficient mice demonstrate the importance of BRP-39 in modulating the severity of clinical experimental autoimmune encephalomyelitis and central nervous system neuroinflammation. PMID: 23041842
    23. Chitinase 3-like-1 is induced by Streptococcus pneumoniae and plays central roles in promoting bacterial clearance and mediating host tolerance. PMID: 22817986
    24. CHI3L1 up-regulates pro-inflammatory mediators, C-chemokine ligand 2, chemokine CX motif ligand 2 and matrix metalloproteinase-9 all of which contribute to tumor growth and metastasis; study shows that CHI3L1 plays a role in tumor progression PMID: 21866546
    25. YKL-40-mediated inhibition of cell differentiation and polarization in the presence of lactogenic hormones may represent its important function during mammary tissue involution. PMID: 21991364
    26. YKL-40/BRP-39 levels are increased in human and murine CF airway fluids PMID: 21949714
    27. The carbohydrate-binding region of CHI3L1 is critical in activating Akt signaling in colon epithelial cells, and the activation may be associated with the development of chronic colitis. PMID: 21546314
    28. breast regression protein-39 has a role in the pathogenesis of cigarette smoke-induced inflammation and emphysema PMID: 20656949
    29. BRP-39 is functionally important in house dust mite-induced inflammation. BRP-39 is a biomarker of cigarette smoke induced inflammation which is the byproduct of an IL-1 inflammatory pathway. PMID: 21473774
    30. pathogenic role in colitis, presumably by enhancing the adhesion and invasion of bacteria on/into colonic epithelial cells. PMID: 16472595
    31. Cloning, expression, characterization and crystallization of BRP39, a signalling glycoprotein expressed during mammary gland apoptosis PMID: 19041398
    32. BRP-39 deficient mice show diminished antigen-induced Th2 cell responses, rescued by YKL-40 administration. PMID: 19414556

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  • 亚细胞定位：
    Secreted, extracellular space. Cytoplasm. Endoplasmic reticulum. Note=Detected in bronchoalveolar lavage (BAL) fluids. Localizes mainly to endoplasmic reticulum when overexpressed in cells, with some protein also detected throughout the cytoplasm.
  • 蛋白家族：
    Glycosyl hydrolase 18 family
  • 组织特异性：
    Detected in lung in pulmonary macrophages and alveolar type 2 cells and in bronchoalveolar lavage (BAL) fluids. Expressed in mammary tumor cells (at protein level). Expressed in lung. Not detected in non-inflammatory colon.
  • 数据库链接：