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  4. Mouse Collagen Type Ⅰ,Col ⅠELISA Kit

Mouse Collagen Type Ⅰ,Col ⅠELISA Kit

  • 中文名称：
    小鼠Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒
  • 货号：
    CSB-E08083m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse Col Ⅰ ELISA Kit was designed for the quantitative measurement of Mouse Col Ⅰ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
  • 缩写：
    Col Ⅰ
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ColⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 92  
    Range % 87-97  
    1:200 Average % 96  
    Range % 91-104  
    1:400 Average % 97  
    Range % 94-101  
    1:800 Average % 98  
    Range % 89-102  
  • 回收率：
    The recovery of mouse ColⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 103 98-107  
    EDTA plasma (n=4) 92 87-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.595 2.494 2.545 2.355  
    5 1.904 1.803 1.854 1.664  
    2.5 1.148 1.128 1.138 0.948  
    1.25 0.660 0.671 0.666 0.476  
    0.625 0.432 0.442 0.437 0.247  
    0.312 0.304 0.315 0.310 0.120  
    0.156 0.249 0.258 0.254 0.064  
    0 0.189 0.190 0.190 　  
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse Col I antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled Col I antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 2 个评价

样品类型：血清

样品信息：小鼠

稀释比：其他 1:10

产品评价： 我用CSB-E08083m检测小鼠血清MCAF水平，试剂盒样本量充足，样本和稀释液颜色对应直观，使用说明简洁明了，操作容易上手，数据准确，满意。

By 刘老师

样品类型：血清

样品信息：小鼠

稀释比：其他 1:10

产品评价： 我用CSB-E08083m检测小鼠血清胶原蛋白水平，结果如图所示，试剂盒样本充足，样本和稀释液颜色对应直观，使用说明简洁明了，操作容易上手，数据准确，满意。

By 刘老师