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  4. Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit

  • 中文名称：
    兔子Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒
  • 货号：
    CSB-E06858Rb
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Rabbit Col Ⅰ ELISA Kit was designed for the quantitative measurement of Rabbit Col Ⅰ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
  • 缩写：
    Col Ⅰ
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-89  
    1:2 Average % 90  
    Range % 85-96  
    1:4 Average % 88  
    Range % 84-92  
    1:8 Average % 104  
    Range % 100-109  
  • 回收率：
    The recovery of rabbit Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-96  
    EDTA plasma (n=4) 99 94-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.013 2.136 2.075 1.935  
    5 1.749 1.705 1.727 1.587  
    2.5 1.388 1.498 1.443 1.303  
    1.25 1.065 1.154 1.110 0.970  
    0.625 0.754 0.788 0.771 0.631  
    0.312 0.513 0.532 0.523 0.383  
    0.156 0.312 0.331 0.322 0.182  
    0 0.139 0.141 0.140    
  • 数据处理：
  • 货期：
    3-5 working days

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