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  4. Mouse Collagen alpha-1(I) chain (COL1A1) ELISA kit

Mouse Collagen alpha-1(I) chain (COL1A1) ELISA kit

  • 中文名称：
    小鼠I型胶原α1链(COL1A1)酶联免疫试剂盒
  • 货号：
    CSB-EL005727MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse COL1A1 ELISA Kit was designed for the quantitative measurement of Mouse COL1A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    Col1a1 ELISA kit; Cola1Collagen alpha-1(I) chain ELISA kit; Alpha-1 type I collagen ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse COL1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 81-92  
    1:2 Average % 102  
    Range % 95-105  
    1:4 Average % 91  
    Range % 85-95  
    1:8 Average % 97  
    Range % 91-101  
  • 回收率：
    The recovery of mouse COL1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 82-92  
    EDTA plasma (n=4) 100 90-104  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.558 2.599 2.579 2.386  
    10 2.008 2.111 2.060 1.867  
    5 1.389 1.342 1.366 1.173  
    2.5 0.843 0.828 0.836 0.643  
    1.25 0.503 0.540 0.522 0.329  
    0.625 0.377 0.384 0.381 0.188  
    0.312 0.278 0.268 0.273 0.080  
    0 0.195 0.191 0.193    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 基因功能参考文献：
    1. Our studies demonstrate that a collagen-I-dense ECM can potently alter hormonal signals to drive the progression of ERalpha + breast cancer, increasing intravasation and pulmonary metastases. PMID: 28103936
    2. Offspring that inherited the osteogenesis imperfect (OI) and high bone mass alleles had higher bone mass and strength than mice that inherited the OI allele alone. PMID: 27297606
    3. a surface population of Hsp90 extracellularly binds TGFbetaRI and this complex behaves as an active participant in collagen production in TGFbeta-activated fibroblasts. PMID: 27418101
    4. Gremlin1 accelerates hepatic stellate cell activation through upregulation of TGF-B1, alpha-SMA, and COL1a1 expression in a liver fibrosis disease model. PMID: 28467108
    5. collagen-I-mediated inhibition of proplatelet formation is specifically controlled by GPVI. PMID: 27505889
    6. Col1a1Jrt/+ mutant mice produce craniofacial and dental defects consistent with osteogenesis imperfecta and Ehlers-Danlos syndrome PMID: 26951553
    7. the rate of collagen I degradation was increased in Poldip2(+/-) vs. Poldip2(+/+) MASMs. Conversely, activation of the PI3K/Akt/mTOR signaling pathway, involved in regulation of protein synthesis, was significantly elevated in Poldip2(+/-) MASMs as was beta1-integrin expression. PMID: 26801741
    8. These data provided a plausible mechanism linking maternal vitamin D deficiency with altered postnatal lung function. PMID: 27121020
    9. Col1 expression was seen in the cartilage zone of the mandibular condyle cartilage. PMID: 26362410
    10. the Col1a1Jrt/+ mouse model of severe OI has hypersensitivity to mechanical and thermal stimuli, consistent with a state of chronic pain PMID: 26277094
    11. HRG binds to alpha2 integrin through low-affinity interactions in a heparin sulfate-independent manner, thereby blocking endothelial cells adhesion to collagen I. PMID: 26051322
    12. Calreticulin in mediates vascular smooth muscle cell responses to injury through the regulation of collagen deposition and neointima formation. PMID: 26910059
    13. Homocysteine Induces Collagen I Expression by Downregulating Histone Methyltransferase G9a PMID: 26192994
    14. These data suggested that intestinal epithelial cells were injured after IM treatment through the pVHL overexpression-induced degradation of collagen I or HIF-1alpha. PMID: 26551465
    15. lipid nanoparticles loaded with small interfering RNA to the procollagen alpha1(I) gene specifically reduce total hepatic collagen content without detectable side effects, potentially qualifying as a therapy for fibrotic liver diseases PMID: 26096209
    16. Collagen Ialpha1 matricryptin p1158/59 facilitates left ventricle remodeling post-myocardial infarction by regulating scar formation through targeted extracellular matrix generation and stimulation of angiogenesis. PMID: 26383724
    17. low-level laser therapy influences the expression of col1a1 and vegf in a mouse fibroblast cell line. PMID: 25171833
    18. Hgh fat diet increased TGFbeta1 protein expression, Smad3 activation, and collagen deposition in skeletal muscle, and exercise attenuated TGFbeta1 protein expression and collagen deposition in skeletal muscle. PMID: 25689619
    19. During heart valve development, Krox20-mediated activation of fibrillar Col1a1 and Col3a1 genes is crucial to avoid postnatal degeneration of the aortic valve leaflets. PMID: 25344368
    20. Data indicate significantly reduced volumetric density of elastin and collagen and thinner skin dermis were observed in Marfan mice. PMID: 25086405
    21. Fibrocytes are not an essential source of type I collagen during lung fibrosis. PMID: 25281715
    22. Allele-specific Col1a1 silencing reduces mutant collagen in fibroblasts from Brtl mouse, a model for classical osteogenesis imperfecta. PMID: 24022296
    23. These findings reveal a novel aging cascade whereby resistance to collagen cleavage accelerates cellular aging. PMID: 23957394
    24. Hypoxic tumor environments exhibit disrupted collagen I fibers and low macromolecular transport. PMID: 24349142
    25. results indicate that Hrd1 plays an important role in the maturation of collagen I in renal fibrosis, and that Sec23A pathway is required for ER-to-Golgi procollagen trafficking to promote collagen synthesis PMID: 24114659
    26. Data indicate a significantly increased expression of type I collagen, TIMP-2, TGF-beta, PAI-1 and RAGE in diabetic db/db cells. PMID: 23991045
    27. Analysis of mutant mouse lines for Col1a1 and Jag1 (Delta-Notch pathway) that both showed clear alterations in several bone-related parameters PMID: 23371561
    28. the absence of integrin alpha2beta1 alleviates the effects of age related bone degradation through over-expression of collagen type I. PMID: 23680479
    29. Data indicate serine-threonine kinase receptor-associated protein (STRAP) as a novel regulator of the coordinated translation of collagen alpha1(I) and alpha2(I) mRNAs. PMID: 23918805
    30. This study deministrated that collagen1alpha1 cells as a novel source of the fibrotic scar after spinal cord injury and shift the focus from the meninges to the vasculature during scar formation. PMID: 23966707
    31. DNA injection and electroporation of the testes showed that spermatogonia self-renewal ability was impaired remarkably with the loss-of-function of Col1a1. PMID: 23064687
    32. Strong transgenerational inheritance of these perturbations was observed only at transgenes integrated at the Col1a1 locus, where both activating and repressive chromatin modifications were heritable for multiple generations. PMID: 23325759
    33. Trps1 is identified as a potent inhibitor of Dspp expression and the subsequent mineralization of dentin. PMID: 22508542
    34. Hypermethylation of CpG sites in the promoter/exon 1 of COL1A1 may underlie reduced collagen synthesis at the transcriptional level in myopic scleras. PMID: 22690110
    35. we show that Prdm5 controls both Collagen I transcription and fibrillogenesis by binding inside the Col1a1 gene body and maintaining RNA polymerase II occupancy PMID: 22589746
    36. Higher MMP2 expression in mice on high-fat diet suggests its involvement in the reduction of COL1A1 protein abundance with high-fat diet. PMID: 21775118
    37. LH3-mediated glucosylation occurs at the specific molecular loci in the type I collagen molecule and plays critical roles in controlling collagen cross-linking, fibrillogenesis, and mineralization. PMID: 22573318
    38. atomistic simulations of a section of mouse type I heterotrimer and homotrimer collagen molecules PMID: 22325288
    39. Adult male offspring of OVA-sensitized /challenged dams exposed to maternal cigarette smokeS (OSM) displayed 1.8-fold greater collagen 1A1 mRNA levels. PMID: 21718087
    40. Fetal microchimeric cells switch on collagen type I transcription implicating mesenchymal lineage cells. PMID: 21150166
    41. study reports that vimentin filaments associate with collagen mRNAs in a 5'stem-loop sequence and LARP6-dependent manner and stabilize collagen mRNAs PMID: 21746880
    42. an important role of the 5' stem-loop in regulating type I collagen mRNA and protein expression PMID: 21193410
    43. Periostin is a PTH regulated extracellular matrix protein, partly co-localizing with collagen 1a1. PMID: 20654714
    44. SPARC is a critical regulator of collagen assembly. The type I collagen N-propeptide can effect the incorporation of collagen I into fibrillar extracellular matrix. PMID: 20708079
    45. Col(I)-Luc transgenic mice can be applied in the field of bone tissue engineering for monitoring bone repair processes and for investigating osteoinductive molecules or scaffolds. PMID: 20218816
    46. Transgenic (col1A1) Col3.6-HSD2 mice showed a low bone mass phenotype, with decreased ex vivo osteogenesis, which strengthens the concept that endogenous glucocorticoid signaling is required for optimal bone mass acquisition PMID: 20541046
    47. Exogenous VEGF restored resistance to oxidative stress in BAV SMCs to normal. Type I collagen gene induction was increased in BAV aorta. PMID: 21095332
    48. Demonstrate that collagen content is important to extralobar pulmonary artery stiffening caused by chronic hypoxia in Col1a1 transgenic mice. PMID: 20852040
    49. Interactions of alpha1(I)(3) homotrimers and normal alpha1(I)(2)alpha2(I) heterotrimers with fibroblast collagenase (MMP-1), were studied. PMID: 20463013
    50. Col1a1 gene regulatory elements have sites of stress-induced DNA duplex destabilization and nuclear scaffold/matrix association potential PMID: 11813254

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibrillar collagen family
  • 组织特异性：
    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 数据库链接：