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  4. Mouse Complement factor I(CFI) ELISA kit

Mouse Complement factor I(CFI) ELISA kit

  • 中文名称：
    小鼠补体因子I(CFI)酶联免疫试剂盒
  • 货号：
    CSB-EL005279MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse CFI ELISA Kit was designed for the quantitative measurement of Mouse CFI protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

  • 别名：
    Cfi ELISA Kit; IfComplement factor I ELISA Kit; EC 3.4.21.45 ELISA Kit; C3B/C4B inactivator) [Cleaved into: Complement factor I heavy chain; Complement factor I light chain] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CFI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 97
    Range % 93-100
    1:2000 Average % 85
    Range % 81-89
    1:4000 Average % 96
    Range % 93-99
    1:8000 Average % 87
    Range % 85-90
  • 回收率：
    The recovery of mouse CFI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 98-106
    EDTA plasma (n=4) 85 81-89
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    50 2.230 2.301 2.266 2.161
    25 2.020 1.987 2.004 1.899
    12.5 1.601 1.700 1.651 1.546
    6.25 1.085 1.125 1.105 1.000
    3.12 0.596 0.568 0.582 0.477
    1.56 0.357 0.361 0.359 0.254
    0.78 0.205 0.212 0.209 0.104
    0 0.102 0.108 0.105 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization.
  • 基因功能参考文献：
    1. Factor I-mediated generation of activated C3 fragments in the circulation is a critical determinant for the development of MPGN2 associated with factor H deficiency. PMID: 18202746
  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Expressed in the liver by hepatocytes. Also present in other cells such as monocytes, fibroblasts or keratinocytes.
  • 数据库链接：