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  4. Mouse Corticosteroid-binding globulin(SERPINA6) ELISA kit

Mouse Corticosteroid-binding globulin(SERPINA6) ELISA kit

  • 中文名称：
    小鼠皮质类固醇结合球蛋白(SERPINA6)酶联免疫试剂盒
  • 货号：
    CSB-EL021062MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse SERPINA6 ELISA Kit was designed for the quantitative measurement of Mouse SERPINA6 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.2 μg/mL-80 μg/mL and the sensitivity is 0.2 μg/mL.

  • 别名：
    Serpina6 ELISA kit; CbgCorticosteroid-binding globulin ELISA kit; CBG ELISA kit; Serpin A6 ELISA kit; Transcortin ELISA kit
  • 缩写：
    SERPINA6
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.2 μg/mL-80 μg/mL
  • 灵敏度：
    0.2 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPINA6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 89-102
    1:2 Average % 95
    Range % 87-105
    1:4 Average % 91
    Range % 83-99
    1:8 Average % 95
    Range % 90-100
  • 回收率：
    The recovery of mouse SERPINA6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 86-105
    EDTA plasma (n=4) 96 89-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average
    0 2.135 2.121 2.128
    0.2 1.528 1.498 1.513
    0.7 0.992 1.013 1.003
    2.5 0.544 0.552 0.548
    15 0.339 0.352 0.346
    80 0.160 0.157 0.159
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
  • 基因功能参考文献：
    1. CBG is involved in the gender differences observed in corticosteroids circulating levels and plays a role in the local regulation of corticosteroids availability in organs like lung. PMID: 26741814
    2. the same gene encodes brain and liver corticosteroid-binding globulin PMID: 24246737
    3. Data suggest fine control interaction between plasma CBG upregulation and stress-induced corticosterone (CORT) release in mouse model of psychological stress; CBG appears to function in maintaining dynamic equilibrium between CBG-bound/-unbound CORT. PMID: 24048966
    4. Sequencing analysis showed that Cbg cDNA obtained from the mouse hypothalamus was homologous to Cbg cDNA obtained from the liver. PMID: 22996440
    5. Cbg-/- mice, unlike controls, remain insensitive to stress applied before memory retrieval. Furthermore, under stress conditions, we observed a blunted surge of corticosterone (CORT) in plasma and no free CORT rise in the hippocampus of Cbg-/-. PMID: 22930537
    6. study describes functional hyporesponsiveness and aldosterone resistance in distal colon of CBG(-/-) mice; steroid actions require an intrinsic but unknown function of CBG, which allows the sufficient supply of the hormone/s to the target tissue PMID: 20969731
    7. Critical role of plasma Cbg in providing an adequate endocrine and behavioral response to stress. PMID: 20022933
    8. These data suggest an active role for CBG in bioavailability, local delivery, and/or cellular signal transduction of glucocorticoids that extends beyond a function as a mere cargo transporter. PMID: 16980625

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 组织特异性：
    Expressed by the liver; secreted in plasma.
  • 数据库链接：