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Mouse Creatine kinase M-type(CKM) ELISA kit

  • 中文名称：
    小鼠M型肌酸激酶(CKM)酶联免疫试剂盒
  • 货号：
    CSB-EL005459MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse CKM ELISA Kit was designed for the quantitative measurement of Mouse CKM protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 1.56 mU/mL-100 mU/mL and the sensitivity is 0.39 mU/mL.

  • 别名：
    Ckm ELISA Kit; Ckmm ELISA Kit; Creatine kinase M-type ELISA Kit; EC 2.7.3.2 ELISA Kit; Creatine kinase M chain ELISA Kit; Creatine phosphokinase M-type ELISA Kit; CPK-M ELISA Kit; M-CK ELISA Kit
  • 缩写：
    CKM
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    1.56 mU/mL-100 mU/mL
  • 灵敏度：
    0.39 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %86-95
    1:2Average %98
    Range %92-100
    1:4Average %90
    Range %87-96
    1:8Average %91
    Range %85-95
  • 回收率：
    The recovery of mouse CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-99
    EDTA plasma (n=4)9995-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    1002.893 3.112 3.003 2.890
    502.323 2.394 2.359 2.246
    251.487 1.555 1.521 1.408
    12.50.902 0.980 0.941 0.828
    6.250.431 0.408 0.420 0.307
    3.120.347 0.321 0.334 0.221
    1.560.248 0.260 0.254 0.141
    00.115 0.110 0.113
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 基因功能参考文献：
    1. We conclude that ILK negatively and independently of PI3K regulated MEF2C phosphorylation activity and MCK mRNA expression in C2C12 cells PMID: 26788505
    2. The Fxn KO/Mck mice tested from one to two months of age showed abnormal gait patterns accompanied by a loss in motor skills. PMID: 25765157
    3. PI3K and AKT regulated skeletal muscle differentiation by regulating the expression of Myogenin and MCK. PMID: 23732671
    4. CK-M overexpression led to significantly increased contractile function at baseline and during adrenergic stimulation and increased survival after thoracic aortic constriction-induced heart failure. PMID: 22201686
    5. strong down-regulation of MCK activity contributes to F-actin instability and induces post-translational modification of alphaB-crystallin and desmin PMID: 21768101
    6. These studies indicate that the age-associated oxidative and nitrative modification of CKm results in a decrease in its activity and may cause structural changes that promote oligomerization and aggregation. PMID: 20195383
    7. Not involved in levels of creatine nnd creatine kinase in skeletal muscle of knockout mice. PMID: 12640020
    8. one of the consequences of M-CK and AK1 deficiency is hampered phosphoryl delivery to the actomyosin ATPase, resulting in a loss of contractile performance. PMID: 12730234
    9. Allosteric activation of phosphorylase b accounts for the accelerated glycogenolysis in CK-/- muscle during contraction. PMID: 12963789
    10. Data show that Six4 is transcriptional regulatory element X (TrexBF) in skeletal myocytes and transactivates the muscle creatine kinase enhancer PMID: 14966291
    11. the expression of muscle creatine kinase (MCK) gene in the heart and skeletal muscle of mice during aging PMID: 15040454
    12. metabolic failure induced by CK deficiency profoundly affects the ability of mice to engage in chronic bouts of endurance running exercise and that this decrease in performance is also associated with muscle wasting PMID: 15831533
    13. CK-MM autoantibodies can modulate the rate of CK clearance from the circulation in myositis PMID: 16810680
    14. Data show that spatial relations among organelles of muscle cells undergo adaptation in response to nonstructural stimuli such as metabolic (creatine kinase)deficiency. PMID: 16855221
    15. The creatine kinase energy transport system was evaluated in the failing mouse heart. PMID: 17481652
    16. Dec proteins repressed a MyoD-activated promoter activity of muscle creatine kinase gene through class A E-box in an HDAC1-independent manner. PMID: 17487425
    17. Creatine kinase (CK) and adenylate kinase (AK) are especially important in facilitating energy metabolism during very high energy demands. PMID: 19156695

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    ATP:guanido phosphotransferase family
  • 数据库链接：

    KEGG: mmu:12715

    STRING: 10090.ENSMUSP00000003643

    UniGene: Mm.2375