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  4. Mouse Eosinophil Peroxidase (EPO) ELISA Kit

Mouse Eosinophil Peroxidase (EPO) ELISA Kit

  • 中文名称：
    小鼠嗜酸性粒细胞过氧化物酶(EPO)酶联免疫试剂盒
  • 货号：
    CSB-E15830m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse EPX ELISA Kit was designed for the quantitative measurement of Mouse EPX protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

  • 别名：
    Epx ELISA Kit; Eper ELISA Kit; Eosinophil peroxidase ELISA Kit; EPO ELISA Kit; EC 1.11.1.7) [Cleaved into: Eosinophil peroxidase light chain; Eosinophil peroxidase heavy chain] ELISA Kit
  • 缩写：
    EPX
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %84-92
    1:2Average %94
    Range %90-98
    1:4Average %99
    Range %95-104
    1:8Average %102
    Range %98-107
  • 回收率：
    The recovery of mouse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9592-98
    EDTA plasma (n=4)9694-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.269 2.298 2.284 2.086
    51.674 1.698 1.686 1.488
    2.51.081 1.098 1.090 0.892
    1.250.729 0.769 0.749 0.551
    0.6250.437 0.457 0.447 0.249
    0.3120.388 0.363 0.376 0.178
    0.1560.283 0.303 0.293 0.095
    00.197 0.198 0.198 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils.
  • 基因功能参考文献：
    1. The expression of MBP-1 and EPX was also required for induced lung expression of IL-4/IL-13 in each setting and, in turn, the induced pulmonary remodeling events and lung dysfunction. PMID: 28515227
    2. EPO-mediated protein carbamylation is promoted during allergen-induced asthma exacerbation, and can both modulate immune responses and trigger a cascade of many of the inflammatory signals present in asthma. PMID: 27587397
    3. The absence of MBP-1 and EPX promoted a concomitant loss of eosinophil lineage-committed progenitors in the marrow, identifying a specific blockade in eosinophilopoiesis as the causative event. PMID: 23736699
    4. HER2 was identified as a novel mediator of eosinophil peroxidase signaling. Eosinophil peroxidase, at noncytotoxic levels, can drive cell-cycle progression and proliferation. PMID: 21454806
    5. Host protection against Brugia pahangi infections was unimpaired in mice deficient in eosinophil peroxidase. PMID: 16299347
    6. Mice deficient for either eosinophil peroxidase or major basic protein on the 129/SvJ background developed significantly higher worm burdens than wild-type mice. PMID: 16926417
    7. post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase PMID: 18694936
    8. The activities of EPO, an eosinophilic secondary granule protein, do not affect the development of allergic pulmonary pathologies in the mouse lung at levels comparable to those observed in humans with asthma. PMID: 11466391

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  • 亚细胞定位：
    Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils.
  • 蛋白家族：
    Peroxidase family, XPO subfamily
  • 数据库链接：

    KEGG: mmu:13861

    STRING: 10090.ENSMUSP00000050497

    UniGene: Mm.1315