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  4. Mouse Fibroblast growth factor 5(FGF5) ELISA kit

Mouse Fibroblast growth factor 5(FGF5) ELISA kit

  • 中文名称：
    小鼠成纤维细胞生长因子5(FGF5)酶联免疫试剂盒
  • 货号：
    CSB-EL008632MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse FGF5 ELISA Kit was designed for the quantitative measurement of Mouse FGF5 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 别名：
    Fgf5 ELISA kit; Fgf-5Fibroblast growth factor 5 ELISA kit; FGF-5 ELISA kit; Heparin-binding growth factor 5 ELISA kit; HBGF-5 ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-103
    1:2 Average % 91
    Range % 86-99
    1:4 Average % 91
    Range % 88-94
    1:8 Average % 101
    Range % 96-106
  • 回收率：
    The recovery of mouse FGF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 87-94
    EDTA plasma (n=4) 106 101-111
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.555 2.633 2.594 2.485
    1000 2.142 2.258 2.200 2.091
    500 1.567 1.614 1.591 1.482
    250 1.033 1.102 1.068 0.959
    125 0.725 0.754 0.740 0.631
    62.5 0.471 0.445 0.458 0.349
    31.25 0.214 0.221 0.218 0.109
    0 0.105 0.112 0.109 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase.
  • 基因功能参考文献：
    1. Recruited CD11b+ CD68+ Kupffer cells/Mphis may play an essential role in steatohepatitis and fibrosis in FGF5 null mice fed with a HFD. Recruitment and activation of bone marrow derived Mphis is the key factor to develop steatohepatitis from simple steatosis. PMID: 27708340
    2. It participates in the progression of hepatic fibrosis. PMID: 24521867
    3. Interaction of mutant genes Fgf5(go-Y), we, and wal changes the duration of hair growth cycles in mice PMID: 22567929
    4. A deletion of a 9.3-kb region in the Fgf5 gene showed insertion of a 498-bp early transposon element long terminal repeat. Results show that long hair mutation of moja/moja mice is caused by Fgf5 disruption mediated by retrotransposon insertion. PMID: 21512271
    5. parathyroid hormone-related protein and fibroblast growth factor-5 regulate the anagen to catagen transition by independent pathways PMID: 12713572
    6. the Fgf5(go) gene is a powerful modifier of mutant genes determining the process of alopecia. PMID: 19534432

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 数据库链接：

    KEGG: mmu:14176

    STRING: 10090.ENSMUSP00000031280

    UniGene: Mm.5055