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  4. Mouse Fibulin-1(FBLN1) ELISA kit

Mouse Fibulin-1(FBLN1) ELISA kit

  • 中文名称：
    小鼠腓骨蛋白-1(FBLN1)酶联免疫试剂盒
  • 货号：
    CSB-EL008452MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse FBLN1 ELISA Kit was designed for the quantitative measurement of Mouse FBLN1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

  • 别名：
    Fbln1Fibulin-1 ELISA kit; FIBL-1 ELISA kit; Basement-membrane protein 90 ELISA kit; BM-90 ELISA kit
  • 缩写：
    FBLN1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FBLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %95
    Range %89-99
    1:2000Average %95
    Range %91-101
    1:4000Average %94
    Range %89-98
    1:8000Average %98
    Range %92-103
  • 回收率：
    The recovery of mouse FBLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-97
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.419 2.319 2.369 2.203
    1001.701 1.601 1.651 1.485
    501.022 1.002 1.012 0.846
    250.664 0.654 0.659 0.493
    12.50.448 0.425 0.437 0.271
    6.250.375 0.365 0.370 0.204
    3.120.287 0.281 0.284 0.118
    00.168 0.164 0.166 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

 Does the kit contain human and/or animal by-products? What is the standard made of?

A:
Thanks for your inquiry!Standard is NS0-derived recombinant mouse MMP7 protein
Standard of kit CSB-EL008452MO is NS0-derived recombinant mouse FBLN1 protein
The kit does not contain any human and/or animal by-products.

靶点详情

  • 功能：
    Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes.
  • 基因功能参考文献：
    1. Fbln1c plays a key role in regulating airway remodelling and inflammation in chronic asthma. PMID: 28862768
    2. The FBLN1/FGF8 interaction may also be involved in the survival of neural crest cell population during development. PMID: 27402846
    3. the findings indicate that Fbln1 normally suppresses proximal OFT EMT preventing proximal cushion hypercellularity and blood cell accumulation. PMID: 25575930
    4. Fbln1 is a new positive modulator of the formation of membranous bone and endochondral bone in the skull PMID: 25201465
    5. fibulin-1 regulates versican-dependent events in ventricular morphogenesis by promoting ADAMTS1 cleavage of versican leading to suppression of trabecular cardiomyocyte proliferation mediated by the ErbB2-Map kinase pathway. PMID: 22183742
    6. short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin, nidogen-1, fibulin-1 and fibulin-2 PMID: 11733994
    7. structural and functional characterization of promoter PMID: 11829738
    8. binding of HPV E6 protein to fibulin-1 PMID: 12200142
    9. fibulin-1 is a new regulator of ADAMTS-1-mediated proteoglycan proteolysis and may play an important role in proteoglycan turnover in tissues where there is overlapping expression PMID: 16061471
    10. A murine placental overgrowth phenotype is associated with elevated Fbln1 transcript levels. PMID: 16338003
    11. Fbln1 is required for the directed migration and survival of cranial neueral crest cells contributing to the development of pharyngeal glands, craniofacial skeleton, cranial nerves, aortic arch arteries, cardiac outflow tract and cephalic blood vessels. PMID: 18538758

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibulin family
  • 组织特异性：
    Detected in most organs (brain, heart, lung, spleen, liver and kidney). Neurons are the predominant source of production in the brain. Not expressed significantly by astrocytes or microglia.
  • 数据库链接：