Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Filaggrin(FLG) ELISA kit

Mouse Filaggrin(FLG) ELISA kit

  • 中文名称：
    小鼠丝聚蛋白(FLG)酶联免疫试剂盒
  • 货号：
    CSB-EL008712MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse FLG ELISA Kit was designed for the quantitative measurement of Mouse FLG protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

  • 别名：
    FlgFilaggrin ELISA Kit; Fragment ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %97
    Range %92-101
    1:4Average %88
    Range %86-95
    1:8Average %90
    Range %86-95
  • 回收率：
    The recovery of mouse FLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-97
    EDTA plasma (n=4)10195-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.290 2.433 2.362 2.197
    1001.503 1.390 1.447 1.282
    500.810 0.871 0.841 0.676
    250.573 0.610 0.592 0.427
    12.50.381 0.366 0.374 0.209
    6.250.288 0.274 0.281 0.116
    3.120.229 0.239 0.234 0.069
    00.163 0.167 0.165
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
  • 亚细胞定位：
    Cytoplasmic granule.
  • 蛋白家族：
    S100-fused protein family
  • 组织特异性：
    Expressed in the granular layer of the epidermis.
  • 数据库链接：