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Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

  • 中文名称：
    小鼠GDNF家族α样受体(GFRAL)酶联免疫试剂盒
  • 货号：
    CSB-EL009383MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse GFRAL ELISA Kit was designed for the quantitative measurement of Mouse GFRAL protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名：
    Gfral ELISA Kit; Gral ELISA Kit; GDNF family receptor alpha-like ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    25 pg/mL-1600 pg/mL
  • 灵敏度：
    6.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFRAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-98
    1:2 Average % 101
    Range % 97-107
    1:4 Average % 94
    Range % 90-98
    1:8 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of mouse GFRAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-100
    EDTA plasma (n=4) 88 84-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 1.859 1.952 1.906 1.766
    800 1.258 1.260 1.259 1.119
    400 0.720 0.734 0.727 0.587
    200 0.466 0.489 0.478 0.338
    100 0.315 0.328 0.322 0.182
    50 0.229 0.235 0.232 0.092
    25 0.172 0.176 0.174 0.034
    0 0.138 0.141 0.140 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
  • 基因功能参考文献：
    1. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
  • 亚细胞定位：
    Cell membrane; Single-pass membrane protein; Extracellular side.
  • 蛋白家族：
    GDNFR family
  • 组织特异性：
    Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.
  • 数据库链接：

    KEGG: mmu:404194

    UniGene: Mm.213862