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  4. Mouse Galectin 3(GAL-3) ELISA Kit

Mouse Galectin 3(GAL-3) ELISA Kit

  • 中文名称：
    小鼠半乳糖凝集素-3(Gal-3)酶联免疫试剂盒
  • 货号：
    CSB-E14296m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse LGALS3 ELISA Kit was designed for the quantitative measurement of Mouse LGALS3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit
  • 缩写：
    LGALS3
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 85-103  
    1:2 Average % 90  
    Range % 82-99  
    1:4 Average % 92  
    Range % 86-100  
    1:8 Average % 92  
    Range % 89-103  
  • 回收率：
    The recovery of mouse GAL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 90-101  
    EDTA plasma (n=4) 97 91-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.852 2.842 2.847 2.703  
    10 2.207 2.280 2.244 2.100  
    5 1.357 1.384 1.371 1.227  
    2.5 0.841 0.862 0.852 0.708  
    1.25 0.475 0.458 0.467 0.323  
    0.625 0.283 0.297 0.290 0.146  
    0.312 0.231 0.244 0.238 0.094  
    0 0.142 0.146 0.144    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
  • 亚细胞定位：
    Cytoplasm. Nucleus. Secreted.
  • 组织特异性：
    The highest levels are found in activated macrophages.
  • 数据库链接：