Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

Mouse Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

  • 中文名称：
    小鼠XIIA组分泌型磷脂酶A2(PLA2G12A)酶联免疫试剂盒
  • 货号：
    CSB-EL018086MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse PLA2G12A ELISA Kit was designed for the quantitative measurement of Mouse PLA2G12A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    Pla2g12a ELISA Kit; Pla2g12 ELISA Kit; Group XIIA secretory phospholipase A2 ELISA Kit; GXII sPLA2 ELISA Kit; sPLA2-XII ELISA Kit; EC 3.1.1.4 ELISA Kit; Phosphatidylcholine 2-acylhydrolase 12A ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %84
    Range %8-89
    1:2Average %98
    Range %94-103
    1:4Average %85
    Range %82-89
    1:8Average %98
    Range %94-102
  • 回收率：
    The recovery of mouse PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8683-89
    EDTA plasma (n=4)9087-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.410 2.324 2.367 2.178
    20001.610 1.684 1.647 1.458
    10001.085 1.034 1.060 0.871
    5000.700 0.732 0.716 0.527
    2500.468 0.478 0.473 0.284
    1250.388 0.398 0.393 0.204
    62.50.260 0.259 0.260 0.071
    00.191 0.187 0.189
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine.
  • 亚细胞定位：
    Secreted. Cytoplasm.
  • 蛋白家族：
    Phospholipase A2 family
  • 数据库链接：