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  4. Mouse Homocysteine(HCY)ELISA Kit

Mouse Homocysteine(HCY)ELISA Kit

  • 中文名称：
    小鼠高半胱氨酸(Hcy)酶联免疫试剂盒
  • 货号：
    CSB-E16551m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse Hcy ELISA Kit was designed for the quantitative measurement of Mouse Hcy protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 nmol/mL-300 nmol/mL and the sensitivity is 1.1 nmol/mL.

  • 别名：
    N/A
  • 缩写：
    Hcy
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4.7 nmol/mL-300 nmol/mL
  • 灵敏度：
    1.1 nmol/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-105  
    1:2 Average % 90  
    Range % 86-96  
    1:4 Average % 94  
    Range % 90-98  
    1:8 Average % 99  
    Range % 95-107  
  • 回收率：
    The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 84 80-88  
    EDTA plasma (n=4) 95 91-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/ml OD1 OD2 Average Corrected  
    300 2.437 2.352 2.395 2.222  
    150 2.075 2.036 2.056 1.883  
    75 1.674 1.604 1.639 1.466  
    37.5 1.079 1.132 1.106 0.933  
    18.75 0.621 0.597 0.609 0.436  
    9.4 0.416 0.459 0.438 0.265  
    4.7 0.297 0.289 0.293 0.120  
    0 0.179 0.167 0.173 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I want to know some information regarding the kit CSB-E16551M:
The kit is labeled as 4.7-300 nmol/ml, which I assume is nmole/ml. Units are notmally expressed in nM, that is nmoles per liter (nmole/L). Could you confirm the unit is correct?

A:
Thanks for your inquiry.
This kit CSB-E16551m is specific to detect Homocysteine. Usually nM is nmol/L，but most literatures use nmol/mL(some literatures use nM, and it is due to habit) .
If you want to use nM, pls convert the unit. Pls let me know if you have any further questions. Thank you.