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  4. Mouse ICOS ligand(ICOSLG) ELISA kit

Mouse ICOS ligand(ICOSLG) ELISA kit

  • 中文名称：
    小鼠ICOS配体(ICOSLG)酶联免疫试剂盒
  • 货号：
    CSB-EL010958MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse ICOSLG ELISA Kit was designed for the quantitative measurement of Mouse ICOSLG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    Icoslg ELISA Kit; B7h2 ELISA Kit; B7rp1 ELISA Kit; IcoslICOS ligand ELISA Kit; B7 homolog 2 ELISA Kit; B7-H2 ELISA Kit; B7-like protein Gl50 ELISA Kit; B7-related protein 1 ELISA Kit; B7RP-1 ELISA Kit; LICOS ELISA Kit; CD antigen CD275 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ICOSLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %88-97
    1:2Average %100
    Range %95-104
    1:4Average %97
    Range %92-110
    1:8Average %89
    Range %84-93
  • 回收率：
    The recovery of mouse ICOSLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)9287-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.417 2.552 2.485 2.322
    20002.104 2.076 2.090 1.927
    10001.450 1.404 1.427 1.264
    5000.765 0.723 0.744 0.581
    2500.439 0.471 0.455 0.292
    1250.321 0.342 0.332 0.169
    62.50.243 0.229 0.236 0.073
    00.169 0.157 0.163
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Did you test whether the ELISA kit detects recombinant mouse ICOSL protein, a positive control?
The reason we're asking you this because two kits we bought from 2 different companies did not capture the recombinant protein (which is very weird) but only their own standards.
Have already tested this binding capability of the kit?

A:
Thanks for your inquiry!
CSB-EL010958MO
This kit is designed accoding to: https://www.uniprot.org/uniprot/Q9JHJ8.
The standard is 293 cell derived recombinant protein.
“recombinant mouse ICOSL protein”you mentioned should be recombinant mouse ICOSLG protein mentioned in the above link. Sorry we are not clear about the elisa kit from other suppliers, but we could provide you with antibody information about this kit.
Capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.
In principle, if the structure of recombinant protein is consistent with that of native protein, it can be detected, but in many cases the expression system used for recombinant protein will be different, so the later expression quality and modifications, etc are different. What's more, it also involves antibody's recognition area. If the expression area of recombinant protein is just in the antibody's recognition area, in principle it can be detected. However, the protein is basically in the presence of spatial structure, and it is the same for recombinant protein. During the process of translation and modification of recombinant proteins at the later stage of expression, there will be alpha helix and folding, forming spatial structure.
So whether it can be detected or not depends on the results of the experiment.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.
  • 基因功能参考文献：
    1. study confirms the importance of ICOSL shedding in ICOS/ICOSL function and expression and it identifies ADAM10 as the most important sheddase for controlling ICOSL levels PMID: 28814605
    2. results showed that monocyte-derived osteoclast (OC)-like cells (MDOCs) express B7h during their differentiation, and that B7h triggering reversibly inhibits OC differentiation and function both in vitro and in vivo PMID: 27798154
    3. study concludse that lymphopenia-driven autoimmunity and the development of Tfh17 cells in Rasgrp1-deficient mice occur independently of CD275(B7-H2) PMID: 27183569
    4. The expression of ICOSL in the cornea and the ICOS-mediated induction of Foxp3+ CD4+ regulatory T cells may contribute to successful corneal allograft survival. PMID: 28002569
    5. this study shows that sICOSL in the serum of healthy donors increases in an age-dependent manner and that the matrix metalloproteinase inhibitor (MMPI) could suppress sICOSL production PMID: 27138044
    6. There was a clearly positive correlation between the presence of IL-17-producing cells and ICOS expression in ICOSL KO mice; it also showed that Th17 was involved in the pathological tissue remodeling in liver fibrosis induced by schistosomiasis. PMID: 25590646
    7. Selective ablation of ICOSL in CD11c+ cells, but not in B cells, dramatically ameliorates kidney and lung inflammation in lupus-prone transgenic mice. PMID: 25786178
    8. restoring control of the T follicular helper-germinal center B-cell axis by blocking the ICOS-ICOSL pathway reduced the development of atherosclerosis and the formation of tertiary lymphoid organs. PMID: 25552357
    9. ICOSL is a molecular linkage between T-B interactional dynamics and positive selection for high-affinity bone-marrow plasma cell formation; study reveals a pathway by which follicular T-helper cells control the quality of long-lived humoral immunity PMID: 25317561
    10. the costimulatory ligand ICOS ligand (ICOSL) is selectively downregulated on the surface of B cells in an ADAM17-specific manner, although it is not proteolitically processed by recombinant ADAM17 in vitro. PMID: 25108021
    11. the B7h-ICOS interaction may modulate the spread of cancer metastases PMID: 24729612
    12. Findings indicate the separable roles of delivery of antigens and ICOS-L by cognate B cells for follicular Th (Tfh) cell maturation and function. PMID: 24610013
    13. Data indicate that the signals provided by ICOSL-expressing B cells to Teff cells and Tfh cells are necessary for the development of arthritis. PMID: 24449576
    14. ICOS-L maintains tolerance at the fetomaternal interface. PMID: 23578385
    15. In cardiac transplantation apoptosis of CD8(+) T cells in recipient draining lymph nodes was enhanced by pretreatment with donor specific transfusion and impaired ICOS/B7h allorecognition. PMID: 23498793
    16. Data suggest that knockdown of Icosl in mouse leukemic cells may significantly enhance graft versus leukemia effect after allogeneic bone marrow transplantation. PMID: 22732700
    17. lineage-specific transgenic expression on dendritic and plasma cells generates different outcome upon ICOS costimulation PMID: 22686515
    18. Results indicate that ICOSLG blockade may enhance cytotoxity in allogeneic mixed lymphocyte-hematologic neoplasm cell reactions. PMID: 21958057
    19. Induction of IL-10 producing regulatory T cells mainly relied on the inducible costimulator ligand (ICOSL)/ICOS axis PMID: 22154192
    20. ICOS/B7h signal plays an important role in direct allorecognition, eliciting allogeneic responses in vitro. PMID: 22172879
    21. Findings suggest that the noncanonical NF-kappaB pathway regulates the development of Tfh cells by mediating ICOSL gene expression in B cells. PMID: 21768353
    22. A role for ICOS costimulation in the maintenance of effector memory but not central memory CD4 T cells. PMID: 21364749
    23. demonstrate that inducible costimulator (ICOS) provides a critical early signal to induce the transcription factor Bcl6, and Bcl6 then induces CXCR5, the canonical feature of follicular helper CD4(+) T cells PMID: 21636296
    24. expression is essential for allergic airway hyperresponsiveness PMID: 21402623
    25. The data suggest that ICOSL plays a significant role in immunoregulation and protective immunity against Chlamydia infections PMID: 20190137
    26. These studies demonstrate that T(R) cells and the ICOS-ICOS-ligand signaling pathway are critically involved in respiratory tolerance and in downregulating pulmonary inflammation in asthma. PMID: 12145647
    27. The involvement of the B7h-Icos costimulatory pathway has been demonstrated in the development and progression of collagen-induced arthritis. PMID: 12370365
    28. ICOS-L is a ligand for ICOS and plays an important functional role in the activation of memory T cells by endothelial cells [review] PMID: 12456022
    29. While interactions of B7RP-1 with ICOS have no effect on the migration of T cells into B cell follicles, they are required for Th1 and Th2 cells to support fulminant B cell responses in vivo. PMID: 12594252
    30. B7-H2 on B cells is important for recruiting T-cell help via its interaction with ICOS and plays a critical role in costimulating humoral immune responses. PMID: 12714510
    31. data suggest that ICOS/B7RP-1 interactions may not affect the organogenesis, but involve in the functional development of Peyer's patches PMID: 12853164
    32. the ICOS-B7h pathway has roles in regulating alloimmune responses in vivo PMID: 12865411
    33. Th cells, when activated with B7h-deficient APC, exhibited reduced proliferation and IL-2 production. PMID: 14615582
    34. ICOS-B7h has a role in transplantation tolerance to MHC class II mismatched skin grafts PMID: 15636609
    35. Experiments with mice genetically engineered for the absence of B7-related protein 1 (B7RP-1KO) demonstrate that efficient T helper 2 (TH2) cell sensitization and effector function occur in the absence of the ICOS-B7RP-1 costimulatory pathway. PMID: 15728513
    36. ICOS-B7h pathway is critical in the activation of effector/memory T cells that are necessary for the progression of chronic rejection. PMID: 15880041
    37. The B7RP-1/ICOS interaction plays an essential role in the development of CXCR-positive helper T cells in vivo, which preferentially migrate to the germinal center B cell zone where they provide cognate help to B cells. PMID: 16081804
    38. ICOSL is upregulated in nephritic glomeruli, where it locally reduces accumulation of T cells and macrophages and attenuates renal injury. PMID: 16540559
    39. critical role played by ICOS-L-expressing and interleukin 10-producing dendritic cells from Chlamydia-infected mice in the infection-mediated inhibition of allergic responses PMID: 16621988
    40. B7h shedding defines a novel immunoregulatory mechanism by which toll-like receptor (TLR)7/8 and TLR9 signals can enhance T cell help during antibody responses. PMID: 16887997
    41. These results suggest that the ICOS/B7RP-1 interaction plays a critical role in the pathogenesis of uveitis. PMID: 17039566
    42. The generated a 3T3 cellular library retrovirally expressing mutants of the murine costimulatory B7h gene. Screening of this unbiased cellular library identified six residues of murine B7h that are critical for binding to the ICOS receptor. PMID: 18294651
    43. a novel mechanism for the regulation of ICOSL expression in vivo and suggest that the ICOS costimulatory pathway is subject to negative feedback regulation by ICOSL down-regulation in response to ICOS expression. PMID: 18390703
    44. Levels of follicular T helper cells and germinal center B cells in two different models of autoimmunity are shown to be dependent on the maintenance of the inducible T-cell co-stimulator (ICOS)/B7-related protein-1 (B7RP-1) pathway. PMID: 19155489
    45. These findings implicate ICOSL-induced IL-10, but not IDO in the regulation of BM-derived pDC function. PMID: 19208362
    46. compared T cell responses of WT, CD28 knockout (KO), B7-H2 KO, and CD28-B7-H2 double KO (DKO) mice in a model of delayed-type hypersensitivity PMID: 19249753
    47. This molecule acts as a costimulatory counterreceptor by augmenting the recall responses of CD8+ T cells. PMID: 11418641

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, BTN/MOG family
  • 组织特异性：
    Isoform 1 highest expression in lymphoid tissues, such as spleen (mostly in the marginal zone), lymph nodes (particularly in the cortex and in both primary and secondary follicles), thymus (predominantly in the medulla) and Peyer patches (mostly in the fo
  • 数据库链接：