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  4. Mouse Inhibin A,INH-A ELISA Kit

Mouse Inhibin A,INH-A ELISA Kit

  • 中文名称：
    小鼠抑制素A(INH-A)酶联免疫试剂盒
  • 货号：
    CSB-E08238M
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 别名：
    Inha ELISA Kit; Inhibin alpha chain ELISA Kit
  • 缩写：
    INH-A
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.5 pg/mL-100 pg/mL
  • 灵敏度：
    0.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse INH-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-89  
    1:2 Average % 92  
    Range % 87-98  
    1:4 Average % 105  
    Range % 101-109  
    1:8 Average % 87  
    Range % 83-90  
  • 回收率：
    The recovery of mouse INH-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 84 80-87  
    EDTA plasma (n=4) 101 98-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    100 0.147 0.149 0.148    
    25 0.301 0.316 0.309    
    8 0.459 0.468 0.464    
    2 0.851 0.827 0.839    
    0.5 1.395 1.405 1.400    
    0 2.468 2.459 2.464    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Inhibin deficient mice are viable but are acutely sensitive to development of gonadal sex-cord stromal tumors.
  • 基因功能参考文献：
    1. These data suggest that activin-follistatin regulation may play a role during the development of Experimental autoimmune epididymo-orchitis. PMID: 28205525
    2. This study tested the hypothesis that inhibins act in an autocrine manner on Leydig cells using a pre-pubertal Leydig cell line, TM3, as a model of immature Leydig cells. PMID: 27465829
    3. The study is the first to identify an intratesticular role for inhibin/inhibin-alpha subunit, demonstrating that a threshold level of this protein is required for normal testis development and to sustain adult somatic testicular cell function. PMID: 25781564
    4. inhibin is a broad regulatory marker in anterior pituitary cells PMID: 24098340
    5. A novel role for GDF9 in causing reproductive defects and suppressing tumor initiation in the Inha(-/-) mouse model. PMID: 23446452
    6. Data indicate that the inhibin-alpha promoter is activated synergistically by Wt1 and Sf1. PMID: 23326390
    7. Inhibin alpha-subunit N terminus interacts with activin type IB receptor to disrupt activin signaling. PMID: 22267736
    8. relationship between inhibin and Sertoli cell activity PMID: 21998670
    9. These data support the role of proconvertase PCSK5 in the processing of ovarian inhibin subunits during folliculogenesis. PMID: 21408162
    10. There are widespread alterations in the transcriptome of gonadotropin-treated Inha-/- granulosa cells, with significant changes in genes involved in extracellular matrix and cell-cell communication. PMID: 20739397
    11. Results demonstrate that RB is not essential for Sertoli cell tumorigenesis in Inha KO mice but that loss of Inha accelerates the infertility phenotype of Rb cKO mice. PMID: 20676395
    12. SMAD 2 is not required for mediating tumorigenic signals of activin in ovarian tumor development caused by loss of inhibin. PMID: 20565978
    13. beta-catenin synergizes with steroidogenic factor-1 to activate the alpha-inhibin promoter through formation of a transcriptional complex PMID: 12732619
    14. inhibin-alpha gene expression is developmentally regulated in the mouse testis PMID: 15055530
    15. Transregulation of LH receptor (LHR) and transcription factor GATA-4, coexpressed during the adrenocortical tumorigenesis of prepubertally gonadectomized transgenic (TG) mice expressing the inhibin alpha-subunit PMID: 15256532
    16. LRH-1 and SF-1 act similarly to increase promoter activity of inhibin alpha. PMID: 16423880
    17. Activin A has opposite effects on glucagon and arx gene expression in alpha-cells compared with beta-cells, a finding that may have relevance during pancreatic endocrine lineage specification and physiological function of the adult islets PMID: 16988001
    18. Gonadal InhA may be a component of the normal endocrine repertoire that regulates bone quality in both the axial and appendicular skeleton. PMID: 17194739
    19. concurrent loss of Rb and inhibins in the ovary will exacerbate tumor formation PMID: 17510234
    20. LH is not required for tumor formation in the absence of inhibins but promotes tumor progression, likely through alterations in serum hormone levels and cell cycle regulators. PMID: 18657590
    21. The expression of inhibin isotypes increases progressively in the testis of mice with increasing postnatal age, suggesting that inhibin is associated with a negative feedback signal for FSH in testicular maturation. PMID: 19043308
    22. analysis of inhibin alpha null mice uncovered a delay in early thymocyte development and decreased thymocyte numbers in vitro, supporting the role of activins and inhibins during T cell development in vivo. PMID: 19338778
    23. These results demonstrated that endocrine effects of inhibin as a negative feedback controller of follicle stimulating hormone production in gonadotropes are dependent on betaglycan expression. PMID: 19524135
    24. Absence of inhibin alpha resulted in advanced follicular maturation. Regulation of the inhibin/activin system is essential for early folliculogenesis in the prepubertal mouse ovary. PMID: 19666016

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 数据库链接：

    KEGG: mmu:16322

    STRING: 10090.ENSMUSP00000040310

    UniGene: Mm.1100