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  4. Mouse Interferon epsilon(IFNE) ELISA kit

Mouse Interferon epsilon(IFNE) ELISA kit

  • 中文名称：
    小鼠干扰素ε(IFNE)酶联免疫试剂盒
  • 货号：
    CSB-EL011049MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse IFNE ELISA Kit was designed for the quantitative measurement of Mouse IFNE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Ifne ELISA Kit; Ifne1 ELISA Kit; Ifnt1Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit; Interferon tau-1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 83-88
    1:2 Average % 94
    Range % 89-98
    1:4 Average % 103
    Range % 97-108
    1:8 Average % 99
    Range % 95-104
  • 回收率：
    The recovery of mouse IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-101
    EDTA plasma (n=4) 89 83-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.271 2.366 2.319 2.143
    500 1.563 1.625 1.594 1.418
    250 0.944 0.956 0.950 0.774
    125 0.582 0.581 0.582 0.406
    62.5 0.382 0.377 0.380 0.204
    31.2 0.274 0.289 0.282 0.106
    15.6 0.220 0.235 0.228 0.052
    0 0.172 0.179 0.176 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral, including HSV-2, and bacterial, including Chlamydia muridarum, genital infections.
  • 基因功能参考文献：
    1. IFN-epsilon is constitutively expressed by cells of the reproductive tract and is inefficiently secreted by fibroblasts and cell lines. PMID: 23951133
    2. IFN-epsilon could promote migration of antigen-specific CD8(+)T cells to the gut. PMID: 22617838
    3. Ifn-epsilon-deficient mice had increased susceptibility to infection of the female reproductive tract by the common sexually transmitted infections (STIs) herpes simplex virus 2 and Chlamydia muridarum. PMID: 23449591
    4. interactions between estradiol and IFN-tau in the context of the overall immune response during autoimmune orchitis PMID: 21787220
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 组织特异性：
    Expressed at very high levels in uterus and, at much lower levels, in ovary and cervix. Very low levels, if any, in other organs. In the endometrium, expressed in the luminal and glandular epithelial cells (at protein level).
  • 数据库链接：