Mouse Interleukin 27,IL-27 ELISA Kit

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9.

1. data suggested that IL-27/IL-27R expression induced by Toxoplasma gondii infection may regulate mast cell-mediated immune response during acute ocular toxoplasmosis in mouse model PMID: 29779048
2. IL-27 has a unique ability to directly act on hematopoietic stem cells and promote their expansion and differentiation into myeloid progenitors in hematologic neoplasms and malaria. (Review) PMID: 29218601
3. Mechanistic studies have identified that hyperlipidemia induces IL-27 production in a TLR4-dependent manner, likely via downregulating LXR expression in dendritic cells. In this case, mice lacking IL-27 do not develop enhanced antibody responses. PMID: 30037367
4. IFN-gamma induces IL-30 production which suppresses IFN-gamma mediated liver inflammation. PMID: 27721424
5. IL-27 signaling in Foxp3(+) Tregs essential for Tregs to control autoimmune inflammation in the central nervous system PMID: 28874534
6. this study indicates that IL-27 secreted by cervical carcinoma cells restricts the angiogenesis in a paracrine manner in the pathogenesis of cervical cancer PMID: 28508429
7. this study has revealed an important role of thymic dendritic cells-derived IL-27 in the regulation of the phenotypic maturation of CD4(+) single positive thymocytes. PMID: 27469302
8. TTP, encoded by Zfp36, degrades p28 to inhibit IL-27 production by macrophages and is thereby a negative regulator of the antitumour response. PMID: 29021521
9. these data infer that endopeptidase mediated gliadin degradation by macrophages and concomitant IL-27 production drive differentiation of splenic gliadin-specific Tr1-like cells in celiac disease PMID: 27579860
10. endogenous levels of IL-27 affected the oncogenic properties of mutant p53 in vivo in a Li-Fraumeni mouse model. Lack of IL-27 signaling led to shortened survival times and increased incidence of osteosarcomas. PMID: 26979394
11. Interleukin-27 is upregulated centrally and peripherally after intracerebral hemorrhage. Interleukin-27 promotes the tissue-protecting functions of neutrophils via, at least partly, the induction of lactoferrin. PMID: 28928459
12. Serum IL-27 levels and cardiac IL-27 mRNA expression were significantly increased after LPS injection compared with control specimens. PMID: 27525353
13. these findings highlight critical roles for IFN-gamma and IL-27 in the mechanism of respiratory syncytial virus-induced exacerbation PMID: 29167232
14. found that during respiratory virus infection the prototypic inflammatory cytokine IL-6 is a critical anti-inflammatory regulator of viral induced immunopathology in the respiratory tract through its induction of IL-27 PMID: 28953978
15. We demonstrate that IL-27-deficiency is linked to increased mucosal presence of ILC2 in a model of inflammatory lung disease. Moreover, IL-27-treatment inhibited ILC2 proliferation and cytokine production and significantly reduced their accumulation in vivo. PMID: 26982595
16. These data suggest a novel role of the sympathetic neuroendocrine system for the modulation of IL-27-dependent acute inflammation. PMID: 28835457
17. results identified a novel mechanism of action of IL-27 on Tregs in the context of cancers. PMID: 24443555
18. These findings provide new evidence that IL-27 plays an important role in the proinflammatory capacity of monocytes and macrophages via enhancing IL-1beta secretion levels triggered by dual LPS-ATP stimulation. PMID: 28377398
19. findings show that PGE2 inhibits IL-27 production in macrophages and in splenic conventional dendritic cells; and identify a novel pathway consisting of signaling through EP2/EP4-->induction of cAMP-->downregulation of IFN regulatory factor 1 expression and binding to the p28 IFN-stimulated response element site PMID: 28062696
20. these data provide the first evidence that IL-27 signals directly to B cells promote GCs and support the role of IL-27 in lupus. PMID: 27619997
21. this study shows that IL-27 plays an important role in immune response and pathogen clearance during infection with Candida parapsilosis PMID: 27259855
22. Inhibition of IL-27p28 also enhanced the suppressive capacity of adoptively transferred CD4(+) nTregs by increasing the stability of Foxp3 expression. PMID: 27488350
23. These data provide evidence of a central role of IL-27 for the control of Th2-mediated allergic diseases. PMID: 27799314
24. These data indicate that endogenous IL-27 limits pathology during parainfluenza virus infection PMID: 28129374
25. our data suggest a previously unrecognized role for IL-27 in regulating epithelial cell proliferation and antiviral host defense during the normal wound healing response. PMID: 28132857
26. recombinant IL-30 showed its biological activity by inhibiting Th17 differentiating from naive CD4(+) T cells. Therefore, this method of express and purifying IL-30 provides novel procedures to facilitate structural and functions studies of IL-30. PMID: 26176652
27. An IL-27/Lag3 axis enhances Foxp3+ regulatory T cell-suppressive function and therapeutic efficacy. PMID: 26013006
28. IL-27 is one of the limited unique cytokines directly acting on hematopoietic stem cells (HSCs) to promote differentiation into myeloid progenitors during emergency myelopoiesis. PMID: 26991425
29. regulates protective immunity against malaria parasites PMID: 26968425
30. These results provide the first evidences that Fli-1 positively regulates LPS-induced IL-27 production in macrophages PMID: 26900987
31. of autocrine/paracrine mechanisms involving IL-27 in adipocytes under conditions of inflammatory stress that may link obesity with inflammatory diseases. PMID: 26638127
32. Increased IL-27 expression in bleomycin-induced pulmonary fibrosis was noted. PMID: 25888222
33. IL-27 induces the endothelial differentiation in cardiac resident stem cells through STAT3/Pim-1 signaling pathway. PMID: 26142823
34. demonstrate that suppression mediated by interferon and IL-27 is a negative feedback mechanism for group 2 innate lymphoid cells function in vivo PMID: 26595888
35. Natural killer cells-dendritic cells interaction elicits a positive feedback loop for IFN-gamma and IL-27 production. PMID: 26347474
36. demonstrate the essential role of IL-27 signaling in regulating immune responses to extracellular protozoan infections PMID: 26222157
37. IL-27 regulates enhanced susceptibility to S. aureus pneumonia following influenza infection, potentially through the induction of IL-10 and suppression of IL-17. PMID: 25651926
38. Importance of IL-27 for the physiological control of immunopathology and the potential value of well-timed IL-27 application to treat life-threatening inflammation during lung infection. PMID: 24809349
39. Most innate immune receptor agonist-based vaccine adjuvants need IL-27 for CD4 and CD8 responses. IL-27 dependency is T-cell-intrinsic, requiring T-cell expression of IL-27Ralpha. It is critical high-affinity T-cell response to subunit immunization. PMID: 25267651
40. Our study has identified a novel proinflammatory role for IL-27 in vivo that promotes Th17 differentiation by inducing Th17-supporting cytokines in APCs. PMID: 24129161
41. IL-27p28 inhibits central nervous system autoimmunity by concurrently antagonizing Th1 and Th17 responses. PMID: 24021664
42. Type I IFN induces IL-10 production in an IL-27-independent manner and blocks responsiveness to IFN-gamma for production of IL-12 and bacterial killing in Mycobacterium tuberculosis-infected macrophages. PMID: 25187652
43. IL-27/WSX-1 regulated PGE2 secretion via STAT1-COX-2 pathway in macrophages and affected helper T cell response in a PGE2-mediated fashion. PMID: 25088998
44. In conclusion, it is suggested that IL-27 may possibly act as an anti-inflammatory cytokine during malaria. PMID: 24522137
45. IL-27 plays a critical role in the parent-to-F1 model of acute graft versus host disease and that blocking IL-27 could have therapeutic relevance PMID: 24700119
46. this report identifies Tyk2 as a prerequisite factor in the molecular networks that are involved in generation of IL-27. PMID: 24604832
47. these data demonstrate that in contrast to viral infections at other sites, IL-27 does not play a proinflammatory role during JHMV-induced encephalomyelitis. PMID: 24890725
48. IL-27 production by CD11c(+) cells was inhibited. PMID: 24337375
49. the findings from the past decade identify IL-27 as a critical immunoregulatory cytokine, especially for T cells, whereas some controversy is fueled by results challenging the view of IL-27 as a classical silencer of inflammation. PMID: 23904441
50. IL-27 suppresses the production of IFN-gamma by CD4+ T cells during blood stage malaria infection by preventing the development of terminally differentiated Th1 cells specifically by restricting IL-12 signals. PMID: 23593003

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Secreted. Note=Poorly secreted without coexpression of EBI3.

IL-6 superfamily

Expressed in macrophages and dendritic cells.

KEGG: mmu:246779

STRING: 10090.ENSMUSP00000054637

UniGene: Mm.222632