Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Interleukin-36 alpha(IL1F6) ELISA kit

Mouse Interleukin-36 alpha(IL1F6) ELISA kit

  • 中文名称：
    小鼠白介素36α(IL1F6)酶联免疫试剂盒
  • 货号：
    CSB-EL011617MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse Il36a ELISA Kit was designed for the quantitative measurement of Mouse Il36a protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Il36a ELISA Kit; Fil1e ELISA Kit; Il1e ELISA Kit; Il1f6 ELISA Kit; Il1h1Interleukin-36 alpha ELISA Kit; FIL1 epsilon ELISA Kit; Interleukin-1 epsilon ELISA Kit; IL-1 epsilon ELISA Kit; Interleukin-1 family member 6 ELISA Kit; IL-1F6 ELISA Kit; Interleukin-1 homolog 1 ELISA Kit; IL-1H1 ELISA Kit
  • 缩写：
    IL1F6
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL1F6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-90  
    1:2 Average % 89  
    Range % 84-95  
    1:4 Average % 99  
    Range % 95-104  
    1:8 Average % 93  
    Range % 85-99  
  • 回收率：
    The recovery of mouse IL1F6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 90-107  
    EDTA plasma (n=4) 88 80-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.591 2.587 2.589 2.486  
    500 2.017 2.046 2.032 1.929  
    250 1.365 1.389 1.377 1.274  
    125 0.799 0.751 0.775 0.672  
    62.5 0.455 0.483 0.469 0.366  
    31.2 0.261 0.243 0.252 0.149  
    15.6 0.169 0.186 0.178 0.075  
    0 0.102 0.104 0.103 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. Induces the production of proinflammatory cytokines, including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23 in bone marrow-derived dendritic cells (BMDCs). Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes. May play a role in proinflammatory effects in the lung: induces the expression of CXCL1 and CXCL2 in the lung, and the expression of TNF-alpha, IL-36c, IL-1A, IL-1B, CXCL1 and CXCL2 in isolated splenic CD11c(+) alveolar macrophages. May be involved in T-cell maturation by stimulating the surface expression of CD40 and modestly CD80 and CD86 in splenic CD11c(+) cells. May be involved in CD4(+) T-cell proliferation. Induces NF-kappa B activation in macrophages.
  • 基因功能参考文献：
    1. The data identify a novel role for IL-36 signaling in colonic inflammation and indicate that the IL-36R pathway may represent a novel target for therapeutic intervention. PMID: 26813344
    2. IL-1 and IL-36 have roles as inflammatory mediators in pustular psoriasis in a mouse model PMID: 28057979
    3. IL-1alpha and IL-36alpha form a self-amplifying inflammatory loop in vivo that in patients with insufficient counter regulatory mechanisms may become hyper-engaged and/or chronic PMID: 26203636
    4. An essential role of C/EBPbeta in the regulation of the Il36A gene via the proximal half-CRE*C/EBP element in response to inflammatory stimuli. PMID: 26066982
    5. IL-36 promotes myeloid cell infiltration, activation, and inflammatory activity in skin PMID: 24829417
    6. IL-36alpha acts as a pro-inflammatory cytokine in the lungs independent of both IL-1alpha and IL-1beta. PMID: 23029241
    7. Interleukin-36 (IL-36) ligands require processing for full agonist (IL-36alpha, IL-36beta, and IL-36gamma) or antagonist (IL-36Ra) activity PMID: 21965679
    8. A critical role of IL-36R ligands in the interface between innate and adaptive immunity, leading to the stimulation of T helper responses. PMID: 21860022
    9. Transcripts for IL-1F5, -1F6, -1F8, and -1F9 are all significantly increased in the involved skin of bitransgenic mice compared with their monotransgenic controls. PMID: 21242515
    10. local overexpression related to the development of tubulointerstitial lesions PMID: 20101239
    11. IL-1F6 can be externalized via a stimulus-coupled mechanism comparable to that used by IL-1beta PMID: 19717513

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 组织特异性：
    Highly expressed in embryonic tissue and in tissues containing epithelial cells. Elevated expression levels are detected in chronic kidney disease; expressed inepithelia from the distal convoluted tubules (DCTs) to the cortical collecting ducts (CCDs) in
  • 数据库链接：