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  4. Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

  • 中文名称：
    小鼠溶酶体Pro-X羧肽酶(PRCP)酶联免疫试剂盒
  • 货号：
    CSB-EL018636MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse PRCP ELISA Kit was designed for the quantitative measurement of Mouse PRCP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 37.5 pg/mL-2400 pg/mL and the sensitivity is 9.37 pg/mL.

  • 别名：
    Prcp ELISA Kit; Lysosomal Pro-X carboxypeptidase ELISA Kit; EC 3.4.16.2 ELISA Kit; Proline carboxypeptidase ELISA Kit; Prolylcarboxypeptidase ELISA Kit; PRCP ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    37.5 pg/mL-2400 pg/mL
  • 灵敏度：
    9.37 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %85-99
    1:2Average %98
    Range %92-103
    1:4Average %93
    Range %89-98
    1:8Average %91
    Range %87-96
  • 回收率：
    The recovery of mouse PRCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-98
    EDTA plasma (n=4)9493-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    24002.542 2.520 2.531 2.377
    12002.017 1.955 1.986 1.832
    6001.427 1.321 1.374 1.220
    3000.722 0.781 0.752 0.598
    1500.465 0.455 0.460 0.306
    750.322 0.346 0.334 0.180
    37.50.249 0.248 0.249 0.095
    00.154 0.153 0.154
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
  • 基因功能参考文献：
    1. Prolylcarboxypeptidase (PRCP) converts AngII to Ang (1-7) but only at an acidic pH. Global PRCP deficiency causes heart and kidney alterations and a moderate rise in BP. PRCP is abundant in the kidney collecting tubules, where the prevailing pH is low. In collecting tubules, PRCP deficiency could result in impaired AngII degradation. Increased AngII at this nephron site stimulates Na reabsorption and increases BP. PMID: 28160049
    2. activity and expression in plasma and lung tissue stays unaffected in lung ischemia-reperfusion injury PMID: 27437782
    3. This study demonistrated that PRCP gene is broadly expressed in the brain. PMID: 24161824
    4. PRCP regulates cell growth, angiogenesis, and the response to vascular injury PMID: 23744584
    5. PRCP is an important regulator of energy and glucose homeostasis since its deletion significantly improves metabolic parameters in mice exposed to both standard chow diet and high-fat diet PMID: 22454290
    6. analysis of non-benzimidazole and brain-penetrant prolylcarboxypeptidase inhibitors PMID: 22079761
    7. This study showed that PRCP mutant mice have a significant decrease in fat mass, although an increase in lean mass was also observed; reduced leptin levels and increased energy expenditure were also found. PMID: 22202165
    8. Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosis. PMID: 21297000
    9. PRCP is an important component of melanocortin signaling and weight maintenance via control of active alpha-MSH1-13 levels. PMID: 19620781

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  • 亚细胞定位：
    Lysosome.
  • 蛋白家族：
    Peptidase S28 family
  • 数据库链接：