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  4. Mouse Macrophage Inflammatory Protein 1β,MIP-1β ELISA kit

Mouse Macrophage Inflammatory Protein 1β,MIP-1β ELISA kit

  • 中文名称：
    小鼠巨噬细胞炎性蛋白1β(MIP-1β/CCL4)酶联免疫试剂盒
  • 货号：
    CSB-E04665m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse CCL4 ELISA Kit was designed for the quantitative measurement of Mouse CCL4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

  • 别名：
    Ccl4 ELISA Kit; Mip1b ELISA Kit; Scya4 ELISA Kit; C-C motif chemokine 4 ELISA Kit; Immune activation protein 2 ELISA Kit; ACT-2 ELISA Kit; ACT2 ELISA Kit; Macrophage inflammatory protein 1-beta ELISA Kit; MIP-1-beta ELISA Kit; Protein H400 ELISA Kit; SIS-gamma ELISA Kit; Small-inducible cytokine A4 ELISA Kit
  • 缩写：
    CCL4
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.12 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human EPHB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.    
    　 　 Sample 　 Serum(n=4) 　    
    1:1 　 Average % 　 102 　    
    　 　 Range % 　 98-106 　    
    1:2 　 Average % 　 85 　    
    　 　 Range % 　 80-90 　    
    1:4 　 Average % 　 100 　    
    　 　 Range % 　 97-103 　    
    1:8 　 Average % 　 86 　    
    　 　 Range % 　 83-89 　    
  • 回收率：
    The recovery of human EPHB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
     
    Sample Type 　 Average % Recovery 　 Range 　    
    Serum (n=5) 　 99 　 95-103 　    
    EDTA plasma (n=4) 　 87 　 83-90 　    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected 　    
    4000 2.409 2.433 2.421 2.262 　    
    2000 1.778 1.659 1.719 1.560 　    
    1000 1.216 1.268 1.242 1.083 　    
    500 0.861 0.835 0.848 0.689 　    
    250 0.616 0.535 0.576 0.417 　    
    125 0.432 0.435 0.434 0.275 　    
    62.5 0.296 0.289 0.293 0.134 　    
    0 0.159 0.158 0.159 　 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Monokine with inflammatory and chemokinetic properties.
  • 基因功能参考文献：
    1. CCL4-CCR5 axis can contribute to breast cancer metastasis to bone by mediating the interaction between cancer cells and fibroblasts in bone cavity. PMID: 27177471
    2. YY1 regulates lung CCL4 transcription in pulmonary tuberculosis. PMID: 26786659
    3. Ccl4 mRNAs increased within 5 h after injury in mouse cortical slices. PMID: 25895671
    4. CCL4 and CCL5 expression was higher in livers of infected WSX-1(-/-) mice than infected WT mice, and hepatic CD4 T cells from WSX-1(-/-) mice expressed higher levels of CCR5 than cells from WT mice PMID: 24244314
    5. CCL4 upregulation was associated with increased Snail expression and downregulation of p53/PTEN in high-grade PIN and prostate cancer PMID: 23878190
    6. These results suggest that MIP-1beta is a novel key mediator, and the peripheral MIP-1beta-CCR5 axis contributes to neuropathic pain. PMID: 22528550
    7. results indicate that MIP-1beta is involved in the recruitment of bone marrow-derived monocyte lineage cells PMID: 21912378
    8. increased responsiveness of murine eosinophils to MIP-1beta is mediated by CCR5 receptor in mice PMID: 12050188
    9. macrophage inflammatory protein-1beta is a hypoxia-induced neutrophil survival factor. PMID: 15630139
    10. MIP-1beta is overexpressed, and VE-cadherin is underexpressed in heart transplant allografts compared with isografts PMID: 15897346
    11. Overproduction of MIP-1beta in chemokine (C-C motif) receptor 5-deficient mice after collagen II-immunization may contribute partially to the occurrence of arthritis PMID: 15967376
    12. The expression and role CXCL12 and CCL4 and their receptors (CXCR4 and CCR5) in regulating thymocyte migration in conjunction with extracellular matrix during acute T. cruzi infection are investigated. PMID: 16637021
    13. ATF3 appears to be part of a control mechanism that limits the amount of CCL4 released by macrophages, preventing excessive inflammation. PMID: 16982098
    14. Antibody neutralization of CCL4 abrogates the ability of T-cells from IL-4-treated NOD mice to transfer protection against type 1 diabetes. PMID: 17327452

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：