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  4. Mouse Macrophage mannose receptor 1(MRC1) ELISA kit

Mouse Macrophage mannose receptor 1(MRC1) ELISA kit

  • 中文名称：
    小鼠巨噬细胞甘露糖受体1(MRC1)酶联免疫试剂盒
  • 货号：
    CSB-EL014782MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse MRC1 ELISA Kit was designed for the quantitative measurement of Mouse MRC1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    Mrc1 ELISA Kit; Macrophage mannose receptor 1 ELISA Kit; MMR ELISA Kit; CD antigen CD206 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MRC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %88
    Range %81-92
    1:2Average %100
    Range %93-104
    1:4Average %90
    Range %85-95
    1:8Average %99
    Range %91-103
  • 回收率：
    The recovery of mouse MRC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9285-96
    EDTA plasma (n=4)10090-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.716 2.578 2.647 2.462
    202.167 2.187 2.177 1.992
    101.451 1.478 1.465 1.280
    50.857 0.823 0.840 0.655
    2.50.536 0.545 0.541 0.356
    1.250.371 0.359 0.365 0.180
    0.6250.274 0.266 0.270 0.085
    00.188 0.182 0.185
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
  • 基因功能参考文献：
    1. findings show that TLR6 and mannose receptor participate as macrophage receptors for Mycobacterium lepraemurium PMID: 28317806
    2. current study shows that Mrc1-/- mice develop less severe dextran sulfate sodium-induced colitis PMID: 26530135
    3. the key roles of the ManR and ASGR in controlling circulating levels of numerous glycoproteins critical for regulating reproductive hormones and blood coagulation. PMID: 27405760
    4. PLA2 may prevent inflammatory responses in cisplatin-induced acute kidney injury by modulating Treg cells and IL-10 through the CD206 mannose receptor. PMID: 25993317
    5. Hepatic CD206-positive macrophages express amphiregulin to promote the immunosuppressive activity of regulatory T cells in HBV infection. PMID: 26216935
    6. CD206-positive pulmonary macrophages have a critical role in triggering inflammatory cascade during endotoxemic lung inflammation PMID: 25447055
    7. These findings suggest that MR plays a major role in Enterococcus faecalis strain EC-12 phagocytosis by the antigen-presenting cells. PMID: 23801521
    8. Genetic ablation of the Mrc1 impaired the intracellular collagen degradation pathway. PMID: 24019537
    9. Report CD44/macrophage mannose receptor as a ligand/receptor pair involved in the process of lymphocyte transit via the lymphatic vasculature. PMID: 23603511
    10. study reports the macrophage response against Leishmania infantum in vivo is characterized by an M2b-like phenotype and C-type lectin receptors signature composed of Dectin-1, mannose receptor (MR)and the DC-SIGN homolog SIGNR3 expression PMID: 23684988
    11. These findings suggest that MRC1 plays an important role in the uptake of various bacteria by trophoblast giant cells and may provide an opportunity for those bacteria to escape from phagosomes. PMID: 23163874
    12. The results suggest that MRC1 plays a critical role in myeloid plasticity, which in turn affects the adaptive immune response and immunopathogenesis during murine neurocysticercosis. PMID: 23319563
    13. CD206 is an pattern recognition receptor in detection of the infective Schistosoma mansoni and modulates IFNgamma production. PMID: 22036898
    14. Data show that MR polyubiquitination mediates the recruitment of p97 toward the endosomal membrane, proving the central role of the endocytic receptor and its ubiquitination in antigen translocation. PMID: 21628571
    15. The mannose receptor (MR) is the primary receptor mediating the internalization of environmental allergen glycoproteins by dendritic cells. Signals originating from the MR and TLR-2 receptors converge with the notch 1 signalling pathway. PMID: 20819091
    16. MR+ macrophages, surrounding early decidual glands, are able to internalize ligands for carbohydrate recognition domain of the receptor, including decidual secretory phase mucin TAG-72. PMID: 15746201
    17. macrophage mannose receptor lectin activities are influenced by glycosylation PMID: 15983039
    18. These data identify a novel function for mannose receptor (MR) during skeletal muscle growth and suggest that myoblast motility may be a key aspect of regulating myotube growth. PMID: 16864654
    19. Study explores Ptgs1 and Mrc1 genes as two new positional candidate genes for allergen-induced airway hyperresponsiveness through comparative sequence analysis and mRNA expression studies of mouse strains with genetically mediated airway responsiveness. PMID: 17454003
    20. The role of the mannose receptor in C. neoformans infection in wild-type and knockout mice is reported. PMID: 18391001
    21. This study provides a profile of MR N-linked glycosylation that will facilitate our understanding of their physiological role on MR biology in vivo. PMID: 19427834

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  • 亚细胞定位：
    Endosome membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Detected in macrophages.
  • 数据库链接：

    KEGG: mmu:17533

    STRING: 10090.ENSMUSP00000028045

    UniGene: Mm.2019