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  4. Mouse Melanoma-derived growth regulatory protein(MIA) ELISA kit

Mouse Melanoma-derived growth regulatory protein(MIA) ELISA kit

  • 中文名称：
    小鼠黑色素瘤源性生长调控蛋白(MIA)酶联免疫试剂盒
  • 货号：
    CSB-EL013798MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse MIA ELISA Kit was designed for the quantitative measurement of Mouse MIA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.17 pg/mL.

  • 别名：
    Mia ELISA Kit; Cdrap ELISA Kit; Mia1 ELISA Kit; Melanoma-derived growth regulatory protein ELISA Kit; Cartilage-derived retinoic acid-sensitive protein ELISA Kit; CD-RAP ELISA Kit; Melanoma inhibitory activity protein ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4.7 pg/mL-300 pg/mL
  • 灵敏度：
    1.17 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %96
    Range %82-99
    1:200Average %101
    Range %97-105
    1:400Average %92
    Range %88-96
    1:800Average %87
    Range %85-89
  • 回收率：
    The recovery of mouse MIA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 104101-107
    EDTA plasma (n=4)9692-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    3002.502 2.563 2.533 2.426
    1502.256 2.212 2.234 2.127
    751.698 1.680 1.689 1.582
    37.50.859 0.899 0.879 0.772
    18.750.514 0.536 0.525 0.418
    9.40.301 0.326 0.314 0.207
    4.70.192 0.172 0.182 0.075
    00.107 0.106 0.107
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
  • 基因功能参考文献：
    1. The effects of MIA/CD-RAP on transcriptional regulation in chondrocytes, through the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis. PMID: 24349210
    2. Attenuated LM expressing MIA. PMID: 22886976
    3. as observed in other knockout models of molecules important for cartilage development and differentiation, viability and functional integrity is reached by remarkable molecular redundancy in MIA/CD-RAP knockout mice. PMID: 24269712
    4. The cartilage protein melanoma inhibitory activity contributes to inflammatory arthritis. PMID: 24287514
    5. Expression analysis of cartilage tissue derived from MIA-/- mice revealed strong downregulation of nuclear RNA-binding protein 54-kDa. PMID: 21368873
    6. MIA/CD-RAP stabilizes cartilage differentiation and inhibits differentiation into bone potentially by regulating signaling processes during differentiation. PMID: 20164682
    7. transcriptional mechanism by which CD-RAP expression is suppressed by IL-1 beta PMID: 12072435
    8. Cdrap/Mia is located between two housekeeping genes PMID: 15028289
    9. DNA promoter segment from -2,251 bp to -2,068 bp of the CD-RAP gene contains elements critical for gene expression. It demonstrates activation or repression of gene expression in vitro and in vivo based on cell type and content of transcription factors. PMID: 16250001
    10. A potential tumor suppressor role of Mia/Cd-rap in pituitary cells. PMID: 17914116
    11. Microarray analysis showed significant reductions in the expression of several proteins in lung misexpressing MIA. PMID: 18342301

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    MIA/OTOR family
  • 组织特异性：
    All malignant melanoma cell lines tested and infrequently in glioma cell lines.
  • 数据库链接：

    KEGG: mmu:12587

    UniGene: Mm.262447