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  4. Mouse Nuclear factor-kappa B,NF-κB ELISA Kit

Mouse Nuclear factor-kappa B,NF-κB ELISA Kit

  • 中文名称：
    小鼠核因子κB(NF-κB)酶联免疫试剂盒
  • 货号：
    CSB-E12108m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    The mouse NF-κB ELISA Kit is engineered for accurate measurement of mouse NF-κB levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the NF-κB content in the sample. The color intensity is positively correlated with NF-κB content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

    NF-κB is a central mediator of inflammation with multiple links to thrombotic processes. It is involved in the regulation of expression of various genes encoding cytokine, chemokines, adhesion molecules, and anti-microbial peptides, thus orchestrating both innate and adaptive immune responses. Additionally, NF-κB plays a crucial role in modulating the survival, activation, and differentiation of innate immune cells and inflammatory T cells. It targets inflammation not only by boosting the synthesis of inflammatory molecules but also by regulating cell proliferation, apoptosis, morphogenesis, and differentiation. Abnormal NF-κB activation leads to aberrant T cell activation, which is related to autoimmune and inflammatory responses.

  • 别名：
    Nfkb1 ELISA Kit; Nuclear factor NF-kappa-B p105 subunit ELISA Kit; DNA-binding factor KBF1 ELISA Kit; EBP-1 ELISA Kit; NF-kappa-B1 p84/NF-kappa-B1 p98 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1) [Cleaved into: Nuclear factor NF-kappa-B p50 subunit] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %86-99
    1:2Average %97
    Range %92-103
    1:4Average %86
    Range %80-89
    1:8Average %90
    Range %83-99
  • 回收率：
    The recovery of mouse NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
    EDTA plasma (n=4)9287-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.176 2.237 2.207 2.004
    101.635 1.642 1.639 1.436
    51.184 1.201 1.193 0.990
    2.50.795 0.813 0.804 0.601
    1.250.601 0.637 0.619 0.416
    0.6250.435 0.446 0.441 0.238
    0.3120.333 0.341 0.337 0.134
    00.201 0.204 0.203 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

我想问一下CSB-E12108m小鼠细胞因子-kappa B (NF-κB) ELISA试剂盒的样本处理方法。我有一个血清样本，但在用于ELISA检测之前，我会将其在56℃的温度下加热30分钟以进行热灭活处理，这会影响我想检测的细胞因子吗？如果我的样本经过预加热处理，灵敏度会如何？

A:
一般高温会使蛋白变性，影响检测；客户提到的56度30min多用于灭活，理论应该是影响不大，不过我们试剂盒一般不需要特殊操作，血清样本直接按说明书方法制备即可； 如果可以的话，建议客户按照说明书推荐的方法。

靶点详情

  • 功能：
    NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. Plays a role in the regulation of apoptosis. Isoform 5, isoform 6 and isoform 7 act as inhibitors of transactivation of p50 NF-kappa-B subunit, probably by sequestering it in the cytoplasm. Isoform 3 (p98) (but not p84 or p105) acts as a transactivator of NF-kappa-B-regulated gene expression. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
  • 基因功能参考文献：
    1. Reducing NF-kappaB activation attenuates PDLIM2 deficiency-exacerbated inflammation and dyslipidemia in Palmitate -treated primary hepatocytes. PMID: 29852170
    2. Chandipura virus infection triggered the activation of signalling pathways mediated by mitogen-activated protein kinases, including p38, JNK 1 and 2, and nuclear factor kappaB. PMID: 30001342
    3. These findings provide mechanistic insight into how polymorphisms that attenuate NFKB1 expression predispose humans to epithelial cancers, highlighting the pro-tumorigenic activity of STAT1 and identifying targetable vulnerabilities in GC. PMID: 29562203
    4. SIRT1 upregulation protects against liver injury induced by a HFD through inhibiting CD36 and the NF-kappaB pathway in mouse Kupffer cells. PMID: 29845302
    5. activation of NF-kappaB pathway is essential for Bacterial lipoprotein tolerance-augmented antimicrobial activity in innate phagocytes and depends primarily on both NOD1 and NOD2. PMID: 28079153
    6. High host NF-kappaB p50 induces murine glioblastoma tumor. PMID: 30030559
    7. NF-kappaB is responsible for Morgana dependent metastasis. PMID: 29158506
    8. study indicates that downregulation of NF-kappaB1 can suppress renal cell carcinoma tumorigenesis by inducing late apoptosis/necrosis PMID: 29212573
    9. TNF-alpha is involved in cardiac PHLPP1 upregulation during reoxygenation, which is mediated by NF-kappaB transcriptional activity PMID: 29940243
    10. Nuclear Factor kappa B (NF-kappaB) pathway in the bone marrow (BM) vascular niche is a critical signalling axis that regulates haematopoietic stem cells (HSCs) function. PMID: 28000664
    11. NF-kappaB and survivin are coordinately up-regulated in GBM patient tumors, and functional inhibition of either protein or BRD4 in in vitro and in vivo models restores sensitivity to EGFR tyrosine kinase inhibitors PMID: 28724615
    12. NF-kappaB1 phosphorylation is regulated by platelet-activating factor in macrophages. PMID: 27554194
    13. Blockade of CD38 diminishes lipopolysaccharide-induced macrophage classical activation and acute kidney injury involving NF-kappaB signaling suppression. PMID: 29080804
    14. NF-kappaBmiR15abFGF/VEGFA axis contributes to the impaired angiogenic capacity of bone marrowmesenchymal stem cells in high fat dietfed mice. PMID: 28944834
    15. Tyr42 phosphorylation of RhoA GTPase promotes tumorigenesis through NF-kappaB. PMID: 28712859
    16. By modulating the translation of IkappaBalpha via the Mnk2-eIF4E pathway, Brd4 provides an additional layer of control for NF-kappaB-dependent inflammatory gene expression and inflammatory response. PMID: 28461486
    17. BCA3 interacts with Rac1 and augments NF-kappaB signaling in vitro, but has no effect on RANKL-induced bone resorption in vivo. PMID: 28791343
    18. TLR4-NF-kappaB signaling pathway was involved in the lipopolysaccharide-induced acute lung injury. PMID: 28886315
    19. This is the first report demonstrating that NF-kappaB signaling may play a key role in compensatory lung growth (CLG). Given its pathway is crucial in tissue regeneration of various organs, NF-kappaB may shed light on identification of molecular triggers or clinically usable key regulators of CLG. PMID: 28679393
    20. Abrogating ClC-3 blunts lipopolysaccharide-induced Inflammation via blocking the TLR4/NF-kappaB pathway. PMID: 27363391
    21. miR-17 approximately 92 family clusters have a role in iNKT cell ontogenesis via modulation of TGF-beta signaling PMID: 27930306
    22. NF-kappaB1-mediated signaling regulates the development of gastric mucosal pathology following TAM administration. PMID: 28726772
    23. that TG2 depletion increases nuclear factor-kappaB (NF-kappaB) signaling PMID: 28522262
    24. LRRK1 physically interacted and potently synergized with CARMA1 to enhance NF-kappaB activation. PMID: 27166870
    25. NLRC5 knockout mice fed with high fat showed accelerated fibrosis and inflammation response by promoting alpha-SMA, Collagen I, Collagen III, TLR4/MyD88, phosphorylated IKKalpha, IkappaBalpha and NF-kappaB expression. PMID: 28499247
    26. Our data suggest that rCC16 suppresses LPS-mediated inflammatory mediator TNF-alpha, IL-6, and IL-8 production by inactivating NF-kappaB and p38 MAPK but not AP-1 in RAW264.7 cells. PMID: 28338974
    27. Our data indicate for the first time that the inflammasome is involved in the inflammatory response and cell death in hypoxia-induced beta cells through the ROS-TXNIP-NLRP3 axis in vitro. This provides new insight into the relationship between hypoxia and inflammation in T2D. PMID: 29278702
    28. Overall, these results suggest that p53 is involved in improving insulin sensitivity of hepatic cells via inhibition of mitogen-activated protein kinases (MAPKs) and NF-kappaB pathways. PMID: 29258820
    29. In conclusion, the results suggest that linarin has anti-osteoclastic effects and may serve as potential modulatory agents for the prevention and treatment of bone loss-associated diseases. PMID: 29269297
    30. The combination therapy of DANFIN with bortezomib dramatically enhanced the apoptosis of multiple myeloma cells and indicated a remarkable anti-tumor effect in a multiple-myeloma xenograft mouse model. PMID: 29284118
    31. A new mouse modelof CLL was created by crossing the Emicro-TCL1 mouse with the previously described p50 knockout mouse4 to study the role of p50 in CLL pathogenesis. The knockout animals had lower leukocyte counts and less disease burden than the wild type, implying a role for p50 in CLL. PMID: 28515090
    32. Results suggest that insufficient hepatocyte growth factor activator inhibitor type 1 (HAI-1) function promotes intestinal carcinogenesis by activating nuclear factor-kappaB (NF-kappaB) signaling. PMID: 27612426
    33. UVB-irradiated or aged mice skin revealed that mTORC2 activity was significantly upregulated which in turn increased Akt activation and Akt-dependent IkappaB kinase alpha (IKKalpha) phosphorylation, and The increased mTORC2 signaling pathway during skin aging were associated to NF-kappaB activation. PMID: 27486771
    34. activation of the NF-kappaB pathway through TNF plays an important role in the dedifferentiation of astrocytes via the re-expression of Oct4. PMID: 26381429
    35. The constitutive elevations of Prdx6 and NF-kappaB during Clonorchis sinensis infection may be associated with more severe persistent hepatobiliary abnormalities mediated by clonorchiasis. PMID: 27554973
    36. SPAK plays a pathogenic role in IgA nephropathy through the activation of NF-kappaB/MAPK signaling pathway. PMID: 27519267
    37. Identification of a PPAR-gamma --> NF-kappaB --> p22phox neuroprotective signaling cascade opens a new avenue for protecting the brain against ischemic insult. PMID: 26108185
    38. interactions of NF-kappaB and N-myc with GLT-1/EAAT2 promoter sequences was significantly elevated in the ipsi-lateral cortex of both adult and old Traumatic brain injury mice. PMID: 26081154
    39. Data show that inhibition of the protease activity of MALT1 might be a strategy to treat inflammatory bowel disease and the NLRP3 inflammasome and NF-kappaB activation are critical components in MALT1 signaling cascades in this disease model. PMID: 27105502
    40. IFN-gamma/TNFalpha trigger the formation of an NF-kappaB/STAT3 complex. PMID: 28264935
    41. Importantly, inhibition of NF-kappaB by QDs displayed promising effects against the viral replication and in vivo bacterial endotoxin-induced inflammatory responses. These data suggest the QDs as potent inhibitors of the NF-kappaB signaling pathway, both in vitro and in vivo. PMID: 27639114
    42. NF-kB mediated upregulation of CADM1 was identified as a mechanism of TNFalpha induced migration. PMID: 26867147
    43. NF-kappaB activation and the downstream expression of inflammatory factors were therefore down-regulated in along an efficient path and ameliorating the damage as a consequence of LPS-induced acute lung injury. PMID: 28778458
    44. FSTL1 is a secreted osteoclastogenic factor that plays a critical role in osteoclast formation via the NF-kappaB and MAPKs signaling pathways. PMID: 27234130
    45. Sustained NF-kappaB inhibition improves insulin sensitivity but is detrimental to muscle health. PMID: 28556540
    46. Prostaglandin E receptor subtype 2 (EP2; Ptger2) deficiency suppressed nuclear factor kappaB (NF-kappaB) activation in intracranial arteries 4 and 8 weeks after intracranial aneurysm induction. PMID: 28174280
    47. Loss of HDAC-mediated repression and gain of NF-kappaB activation underlie cytokine induction in ARID1A- and PIK3CA-mutation-driven ovarian cancer. PMID: 27681437
    48. Absence of Musashi2 in osteoclast precursors promotes apoptosis and inhibits RANKL-induced NF-kappaB activation, which is essential for osteoclast survival. PMID: 27441652
    49. Study demonstrated that activation of NF-kB in astrocytes contributes not only to white matter demyelination and axonal loss during chronic hypoxia but that an astrocytic pro-inflammatory pathway may have important consequences for cognitive outcome in neurodegenerative disease. PMID: 27487766
    50. NF-kappaB-mediated miR-130a modulation is critical in lung vascular remodeling. PMID: 28755990

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).; [Isoform 5]: Cytoplasm.; [Isoform 6]: Nucleus. Cytoplasm.; [Isoform 7]: Nucleus.
  • 数据库链接：