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  4. Mouse Orexin A ELISA Kit

Mouse Orexin A ELISA Kit

  • 中文名称：
    小鼠阿立新A(Orexin A)酶联免疫试剂盒
  • 货号：
    CSB-E08861m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Mouse Orexin A ELISA Kit is a powerful tool for quantitative analysis of Orexin A protein levels in mouse serum, plasma, tissue homogenates, and cell lysates. Orexin A, also known as hypocretin-1, is a neuropeptide hormone that plays a crucial role in regulating sleep-wake cycles, appetite, and energy metabolism.

    The Mouse Orexin A ELISA Kit has a wide detection range of 78 pg/mL-5000 pg/mL and a high sensitivity of 19.5 pg/mL, ensuring precise and accurate measurements even at low concentrations. The kit uses a sandwich assay principle, which involves the use of two monoclonal antibodies specific to the Orexin A protein.

    The assay time for the kit is 1-5 hours, and the sample volume required is 50-100ul. Detection is done at a wavelength of 450 nm, making it easy to read and interpret the results. The kit is suitable for use in metabolism research.

  • 别名：
    Hcrt ELISA Kit; Ox ELISA Kit; Ppox ELISA Kit; Orexin ELISA Kit; Hypocretin ELISA Kit; Hcrt) [Cleaved into: Orexin-A ELISA Kit; Hypocretin-1 ELISA Kit; Hcrt1); Orexin-B ELISA Kit; Hypocretin-2 ELISA Kit; Hcrt2)] ELISA Kit
  • 缩写：
    Orexin A
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse orexin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 94
    Range % 87-98
    1:2 Average % 103
    Range % 94-107
    1:4 Average % 94
    Range % 82-98
    1:8 Average % 104
    Range % 96-108
  • 回收率：
    The recovery of mouse orexin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 105 96-109
    EDTA plasma (n=4) 100 89-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    5000 2.489 2.413 2.451 2.284
    2500 2.039 1.982 2.011 1.844
    1250 1.461 1.500 1.481 1.314
    625 1.064 1.018 1.041 0.874
    312 0.693 0.666 0.680 0.513
    156 0.508 0.525 0.517 0.350
    78 0.304 0.314 0.309 0.142
    0 0.168 0.166 0.167 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
  • 基因功能参考文献：
    1. The results of this study suggested that hypocretin deficient mice may display signs of resilience to cocaine addiction. PMID: 29454841
    2. Noradrenergic neurons in the locus coeruleus (NA(LC) neurons) projecting to the lateral amygdala (LA) receive synaptic input from orexin neurons. PMID: 29151577
    3. Hcrt loss in Hcrt(ko/ko) mice causes impaired TDW maintenance in baseline wake and blunted delta power in SWS, reproducing, respectively, narcolepsy excessive daytime sleepiness and poor sleep quality PMID: 28630298
    4. The results of this study suggest that the expression of orexin peptides precedes the maturation of electrophysiological activity of orexin neurons. The function of orexin neurons gradually matures by 3 weeks after birth. PMID: 28608460
    5. This study demonstrated that the Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons. PMID: 28966976
    6. Furthermore, immunohistochemical analysis revealed that linalool activated hypothalamic orexin neurons, one of the key mediators for pain processing. Formalin tests in orexin neuron-ablated and orexin peptide-deficient mice showed orexinergic transmission was essential for linalool odour-induced analgesia. PMID: 27845440
    7. The findings of this study suggest an important role of orexin in brain areas involved in anxiety PMID: 27965038
    8. In ORX-KO mice, the ultradian episodic changes in locomotor activity, EEG arousal indices and body temperature were significantly attenuated, but the ultradian patterning was preserved. Findings suggest that the ultradian pattern is common across species and that orexin contributes to driving ultradian episodic changes, however, this neuropeptide is not essential for ultradian pattern generation. PMID: 27491480
    9. OXA and OX1R are expressed in the testis and that they may help in proliferation and development of germ cells, Leydig cells and Sertoli cells, and in the spermatogenic process and steroidogenesis. PMID: 27174745
    10. Results thus suggest that type 1 diabetes mellitus impairs testicular steroidogenesis and glucose homeostasis through inhibition of orexin A/OX1R signaling cascade due to decreased OX1R expression in adult mice, thereby affecting germ cell survival and their proliferation in the testis. PMID: 28690152
    11. Binding of OXA to OX1R is important for the development of the testis. PMID: 26562300
    12. Results suggest that orexin A bidirectionally modulates hippocampal CA1 synaptic plasticity, inhibiting long-term potentiation via OX1Rs at moderate concentrations while inducing re-potentiation via OX1Rs and OX2Rs, possibly through phospholipase C and adenylyl cyclase-protein kinase A signaling at sub-nanomolar concentrations PMID: 26965217
    13. DYN-A(2-17) interacts with orexin A in the paraventricular hypothalamic nucleus to modulate food intake in mice. PMID: 26654796
    14. Awake dynamics and brain-wide direct inputs of hypothalamic MCH and orexin/hypocretin networks have been discovered. PMID: 27102565
    15. Histamine Transmission Modulates the Phenotype of Murine Narcolepsy Caused by Orexin Neuron Deficiency PMID: 26474479
    16. observation that inhibition of the Orexin-1 receptor signaling blunted binge-like Ethanol, and saccharin drinking suggests that reward-related Orexin circuits participate in the consumption of salient reinforcers regardless of calories. PMID: 25623402
    17. Results suggest that exercise produces antidepressant effects via suppression of Hcrt/Orx and melanin-concentrating hormone neural systems in the basolateral amygdala PMID: 25917762
    18. Hcrt neuronal activity and Hcrt-mediated stress responses were inhibited by the satiety hormone leptin. PMID: 25695914
    19. This study demonstrated that the GABAergic input property of orexin neurons responds rapidly to sleep deprivation. PMID: 25286384
    20. sleep deprivation or increasing wakefulness by rescue of orexinergic neurons in APP/PS1 mice lacking orexin increased the amount of amyloid beta pathology in the brain. PMID: 25422493
    21. Our results provide evidence for the first time that the orexin A-induced anxiety-like behavior is mediated through GABA-A-ergic neurotransmissions PMID: 24874709
    22. orexin activates the mTOR pathway, a central regulator of cell growth and metabolism, in the mouse brain and cell lines that express GPCRs, OX1R, or OX2R PMID: 25278019
    23. This study demonistrated that absence of the Hcrt peptide has gender-specific effects. In contrast, Hcrt-ataxin mice and human narcoleptics, with loss of the whole Hcrt cell, show weight gain in both sexes. PMID: 25066943
    24. These results suggest that orexin neuronal activity plays a crucial role in the maintenance of wakefulness especially in the active phase in mice. PMID: 23707248
    25. 351 narcoleptic episodes of orexin-deficient mice were behaviorally characterized PMID: 24337939
    26. our data show that orexin in general is not involved in the animal's acute stress response PMID: 23958928
    27. These findings identify a unique cellular process by which orexin can occlude the reward threshold-elevating effects of coreleased dynorphin and thereby act in a permissive fashion to facilitate reward. PMID: 24706819
    28. Data provide preliminary evidence of a critical role of hypocretin deficiency in pregnancy. PMID: 24772478
    29. hypothalamic orexin system is the key for inhibiting the exacerbating link between depression and type 2 diabetes PMID: 23510906
    30. These observations suggest the importance of neurotensin in maintaining activity of orexin neurons. PMID: 23620827
    31. more severe depressive behavior was associated with reduced hippocampal orexin expression, contrasted with increased orexin plus Orx1 receptor mRNA expression in the amygdala. PMID: 23398442
    32. The mechanism underlying Hcrt-mediated neuroprotection includes attenuation of inflammatory responses after ischemic insult. PMID: 23349191
    33. Hcrt has been implicated in cognitive functions and social interaction so we tested the hypothesis that Hcrt neurons are critical to social interaction, particularly social memory. PMID: 23516292
    34. Following cortical impact, mice exhibit reduced wakefulness and motor activity, when correlations between orexin and these measures are diminished. PMID: 22607167
    35. Mechanism for Hypocretin-mediated sleep-to-wake transitions PMID: 22955882
    36. Hypocretin/orexin neurons are capable of fast glutamatergic control of their projection targets by inducing changes in signals generated by histamine neurons. PMID: 22956835
    37. chronic lack of HCRT signaling may cause subtle alterations in the control of heart period during spontaneous behavior. Conversely, HCRT signaling is not necessary for physiological sleep-dependent changes in systolic blood pressure variability. PMID: 22357806
    38. data demonstrate that hypocretin signaling acting on Hcrtr-1 in the PVN plays a crucial role in the expression of nicotine withdrawal. PMID: 21831361
    39. Angiotensin II reduces food intake by altering orexigenic neuropeptide expression (orexin and NPY) in the mouse hypothalamus PMID: 22234465
    40. Food hoarding also increased orexin-ir/Fos-ir labeling in the lateral hypothalamus PMID: 22046281
    41. Orexin plays an integral role in adaptive thermogenesis and body weight regulation via effects on brown adipose tissue differentiation and function. PMID: 21982708
    42. This study demonistrated that hypocretin (orexin) neurons in brain play the role in operant reinforcement in diurnal phase. PMID: 22031892
    43. this study demonistrated that the role of orexin neurons in the regulation of sleep/wakefulness and may be relevant to the mechanisms. PMID: 21775598
    44. Mice with single deletions of orexin, leptin, neuropeptide Y, and ghrelin, were examined to assess the behavioral changes accompanying calorie restriction. PMID: 21464907
    45. Orexin A produces a robust dose-dependent excitation of the acid-sensitive retrotrapezoid nucleus neurons. PMID: 21145990
    46. These results demonstrate, for the first time, pronounced circadian control of orexin neuron activation and implicate recruitment of orexin cells in dark pulse resetting of the suprachiasmatic nuclei circadian clock. PMID: 19055781
    47. Results suggest that orexin deficiency may lower the satiety threshold resulting in reduced sucrose intake, without altering food intake. PMID: 21086064
    48. Results suggest that endogenous orexinergic activity should be appropriately regulated for normal maintenance of sleep states. PMID: 20711757
    49. Orexin neurons are indispensable for stress-induced thermogenesis in mice. PMID: 20807795
    50. play a key role in the regulation of muscle glucose metabolism associated with highly motivated behavior by activating muscle sympathetic nerves and beta(2)-adrenergic signaling PMID: 19945404

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  • 亚细胞定位：
    Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse.
  • 蛋白家族：
    Orexin family
  • 组织特异性：
    Restricted to neuronal cell bodies of the dorsal and lateral hypothalamus.
  • 数据库链接：